Differential Gene Expression of Three Mastitis-Causing <named-content content-type="genus-species">Escherichia coli</named-content> Strains Grown under Planktonic, Swimming, and Swarming Culture Conditions

ABSTRACT Bacterial motility is thought to play an important role in virulence. We have previously shown that proficient bacterial swimming and swarming in vitro is correlated with the persistent intramammary infection phenotype observed in cattle. However, little is known about the gene regulation d...

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Autores principales:	John D. Lippolis, Brian W. Brunelle, Timothy A. Reinhardt, Randy E. Sacco, Tyler C. Thacker, Torey P. Looft, Thomas A. Casey
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	motility pathogenesis virulence Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/bb21f72e0e1540048471c0d5cbc3b9fc
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spelling	oai:doaj.org-article:bb21f72e0e1540048471c0d5cbc3b9fc2021-12-02T18:15:43ZDifferential Gene Expression of Three Mastitis-Causing <named-content content-type="genus-species">Escherichia coli</named-content> Strains Grown under Planktonic, Swimming, and Swarming Culture Conditions10.1128/mSystems.00064-162379-5077https://doaj.org/article/bb21f72e0e1540048471c0d5cbc3b9fc2016-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00064-16https://doaj.org/toc/2379-5077ABSTRACT Bacterial motility is thought to play an important role in virulence. We have previously shown that proficient bacterial swimming and swarming in vitro is correlated with the persistent intramammary infection phenotype observed in cattle. However, little is known about the gene regulation differences important for different motility phenotypes in Escherichia coli. In this work, three E. coli strains that cause persistent bovine mastitis infections were grown in three media that promote different types of motility (planktonic, swimming, and swarming). Using whole-transcriptome RNA sequencing, we identified a total of 935 genes (~21% of the total genome) that were differentially expressed in comparisons of the various motility-promoting conditions. We found that approximately 7% of the differentially expressed genes were associated with iron regulation. We show that motility assays using iron or iron chelators confirmed the importance of iron regulation to the observed motility phenotypes. Because of the observation that E. coli strains that cause persistent infections are more motile, we contend that better understanding of the genes that are differentially expressed due to the type of motility will yield important information about how bacteria can become established within a host. Elucidating the mechanisms that regulate bacterial motility may provide new approaches in the development of intervention strategies as well as facilitate the discovery of novel diagnostics and therapeutics. IMPORTANCE Bacteria can exhibit various types of motility. It is known that different types of motilities can be associated with virulence. In this work, we compare gene expression levels in bacteria that were grown under conditions that promoted three different types of E. coli motility. Better understanding of the mechanisms of how bacteria can cause an infection is an important first step to better diagnostics and therapeutics.John D. LippolisBrian W. BrunelleTimothy A. ReinhardtRandy E. SaccoTyler C. ThackerTorey P. LooftThomas A. CaseyAmerican Society for MicrobiologyarticlemotilitypathogenesisvirulenceMicrobiologyQR1-502ENmSystems, Vol 1, Iss 4 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	motility pathogenesis virulence Microbiology QR1-502
spellingShingle	motility pathogenesis virulence Microbiology QR1-502 John D. Lippolis Brian W. Brunelle Timothy A. Reinhardt Randy E. Sacco Tyler C. Thacker Torey P. Looft Thomas A. Casey Differential Gene Expression of Three Mastitis-Causing <named-content content-type="genus-species">Escherichia coli</named-content> Strains Grown under Planktonic, Swimming, and Swarming Culture Conditions
description	ABSTRACT Bacterial motility is thought to play an important role in virulence. We have previously shown that proficient bacterial swimming and swarming in vitro is correlated with the persistent intramammary infection phenotype observed in cattle. However, little is known about the gene regulation differences important for different motility phenotypes in Escherichia coli. In this work, three E. coli strains that cause persistent bovine mastitis infections were grown in three media that promote different types of motility (planktonic, swimming, and swarming). Using whole-transcriptome RNA sequencing, we identified a total of 935 genes (~21% of the total genome) that were differentially expressed in comparisons of the various motility-promoting conditions. We found that approximately 7% of the differentially expressed genes were associated with iron regulation. We show that motility assays using iron or iron chelators confirmed the importance of iron regulation to the observed motility phenotypes. Because of the observation that E. coli strains that cause persistent infections are more motile, we contend that better understanding of the genes that are differentially expressed due to the type of motility will yield important information about how bacteria can become established within a host. Elucidating the mechanisms that regulate bacterial motility may provide new approaches in the development of intervention strategies as well as facilitate the discovery of novel diagnostics and therapeutics. IMPORTANCE Bacteria can exhibit various types of motility. It is known that different types of motilities can be associated with virulence. In this work, we compare gene expression levels in bacteria that were grown under conditions that promoted three different types of E. coli motility. Better understanding of the mechanisms of how bacteria can cause an infection is an important first step to better diagnostics and therapeutics.
format	article
author	John D. Lippolis Brian W. Brunelle Timothy A. Reinhardt Randy E. Sacco Tyler C. Thacker Torey P. Looft Thomas A. Casey
author_facet	John D. Lippolis Brian W. Brunelle Timothy A. Reinhardt Randy E. Sacco Tyler C. Thacker Torey P. Looft Thomas A. Casey
author_sort	John D. Lippolis
title	Differential Gene Expression of Three Mastitis-Causing <named-content content-type="genus-species">Escherichia coli</named-content> Strains Grown under Planktonic, Swimming, and Swarming Culture Conditions
title_short	Differential Gene Expression of Three Mastitis-Causing <named-content content-type="genus-species">Escherichia coli</named-content> Strains Grown under Planktonic, Swimming, and Swarming Culture Conditions
title_full	Differential Gene Expression of Three Mastitis-Causing <named-content content-type="genus-species">Escherichia coli</named-content> Strains Grown under Planktonic, Swimming, and Swarming Culture Conditions
title_fullStr	Differential Gene Expression of Three Mastitis-Causing <named-content content-type="genus-species">Escherichia coli</named-content> Strains Grown under Planktonic, Swimming, and Swarming Culture Conditions
title_full_unstemmed	Differential Gene Expression of Three Mastitis-Causing <named-content content-type="genus-species">Escherichia coli</named-content> Strains Grown under Planktonic, Swimming, and Swarming Culture Conditions
title_sort	differential gene expression of three mastitis-causing <named-content content-type="genus-species">escherichia coli</named-content> strains grown under planktonic, swimming, and swarming culture conditions
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/bb21f72e0e1540048471c0d5cbc3b9fc
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Differential Gene Expression of Three Mastitis-Causing <named-content content-type="genus-species">Escherichia coli</named-content> Strains Grown under Planktonic, Swimming, and Swarming Culture Conditions

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