Characterizing rumen microbiota and CAZyme profile of Indian dromedary camel (Camelus dromedarius) in response to different roughages

Abstract In dromedary camels, which are pseudo-ruminants, rumen or C1 section of stomach is the main compartment involved in fiber degradation, as in true ruminants. However, as camels are adapted to the harsh and scarce grazing conditions of desert, their ruminal microbiota makes an interesting tar...

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Autores principales:	Ankit T. Hinsu, Nilam J. Tulsani, Ketankumar J. Panchal, Ramesh J. Pandit, Basanti Jyotsana, Nishant A. Dafale, Niteen V. Patil, Hemant J. Purohit, Chaitanya G. Joshi, Subhash J. Jakhesara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bb22f05e10ff40aea88747e8c0ed6092
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spelling	oai:doaj.org-article:bb22f05e10ff40aea88747e8c0ed60922021-12-02T16:55:54ZCharacterizing rumen microbiota and CAZyme profile of Indian dromedary camel (Camelus dromedarius) in response to different roughages10.1038/s41598-021-88943-92045-2322https://doaj.org/article/bb22f05e10ff40aea88747e8c0ed60922021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88943-9https://doaj.org/toc/2045-2322Abstract In dromedary camels, which are pseudo-ruminants, rumen or C1 section of stomach is the main compartment involved in fiber degradation, as in true ruminants. However, as camels are adapted to the harsh and scarce grazing conditions of desert, their ruminal microbiota makes an interesting target of study. The present study was undertaken to generate the rumen microbial profile of Indian camel using 16S rRNA amplicon and shotgun metagenomics. The camels were fed three diets differing in the source of roughage. The comparative metagenomic analysis revealed greater proportions of significant differences between two fractions of rumen content followed by diet associated differences. Significant differences were also observed in the rumen microbiota collected at different time-points of the feeding trial. However, fraction related differences were more highlighted as compared to diet dependent changes in microbial profile from shotgun metagenomics data. Further, 16 genera were identified as part of the core rumen microbiome of Indian camels. Moreover, glycoside hydrolases were observed to be the most abundant among all Carbohydrate-Active enzymes and were dominated by GH2, GH3, GH13 and GH43. In all, this study describes the camel rumen microbiota under different dietary conditions with focus on taxonomic, functional, and Carbohydrate-Active enzymes profiles.Ankit T. HinsuNilam J. TulsaniKetankumar J. PanchalRamesh J. PanditBasanti JyotsanaNishant A. DafaleNiteen V. PatilHemant J. PurohitChaitanya G. JoshiSubhash J. JakhesaraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ankit T. Hinsu Nilam J. Tulsani Ketankumar J. Panchal Ramesh J. Pandit Basanti Jyotsana Nishant A. Dafale Niteen V. Patil Hemant J. Purohit Chaitanya G. Joshi Subhash J. Jakhesara Characterizing rumen microbiota and CAZyme profile of Indian dromedary camel (Camelus dromedarius) in response to different roughages
description	Abstract In dromedary camels, which are pseudo-ruminants, rumen or C1 section of stomach is the main compartment involved in fiber degradation, as in true ruminants. However, as camels are adapted to the harsh and scarce grazing conditions of desert, their ruminal microbiota makes an interesting target of study. The present study was undertaken to generate the rumen microbial profile of Indian camel using 16S rRNA amplicon and shotgun metagenomics. The camels were fed three diets differing in the source of roughage. The comparative metagenomic analysis revealed greater proportions of significant differences between two fractions of rumen content followed by diet associated differences. Significant differences were also observed in the rumen microbiota collected at different time-points of the feeding trial. However, fraction related differences were more highlighted as compared to diet dependent changes in microbial profile from shotgun metagenomics data. Further, 16 genera were identified as part of the core rumen microbiome of Indian camels. Moreover, glycoside hydrolases were observed to be the most abundant among all Carbohydrate-Active enzymes and were dominated by GH2, GH3, GH13 and GH43. In all, this study describes the camel rumen microbiota under different dietary conditions with focus on taxonomic, functional, and Carbohydrate-Active enzymes profiles.
format	article
author	Ankit T. Hinsu Nilam J. Tulsani Ketankumar J. Panchal Ramesh J. Pandit Basanti Jyotsana Nishant A. Dafale Niteen V. Patil Hemant J. Purohit Chaitanya G. Joshi Subhash J. Jakhesara
author_facet	Ankit T. Hinsu Nilam J. Tulsani Ketankumar J. Panchal Ramesh J. Pandit Basanti Jyotsana Nishant A. Dafale Niteen V. Patil Hemant J. Purohit Chaitanya G. Joshi Subhash J. Jakhesara
author_sort	Ankit T. Hinsu
title	Characterizing rumen microbiota and CAZyme profile of Indian dromedary camel (Camelus dromedarius) in response to different roughages
title_short	Characterizing rumen microbiota and CAZyme profile of Indian dromedary camel (Camelus dromedarius) in response to different roughages
title_full	Characterizing rumen microbiota and CAZyme profile of Indian dromedary camel (Camelus dromedarius) in response to different roughages
title_fullStr	Characterizing rumen microbiota and CAZyme profile of Indian dromedary camel (Camelus dromedarius) in response to different roughages
title_full_unstemmed	Characterizing rumen microbiota and CAZyme profile of Indian dromedary camel (Camelus dromedarius) in response to different roughages
title_sort	characterizing rumen microbiota and cazyme profile of indian dromedary camel (camelus dromedarius) in response to different roughages
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bb22f05e10ff40aea88747e8c0ed6092
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Characterizing rumen microbiota and CAZyme profile of Indian dromedary camel (Camelus dromedarius) in response to different roughages

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