Two lamprey Hedgehog genes share non-coding regulatory sequences and expression patterns with gnathostome Hedgehogs.

Hedgehog (Hh) genes play major roles in animal development and studies of their evolution, expression and function point to major differences among chordates. Here we focused on Hh genes in lampreys in order to characterize the evolution of Hh signalling at the emergence of vertebrates. Screening of...

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Autores principales:	Shungo Kano, Jin-Hua Xiao, Joana Osório, Marc Ekker, Yavor Hadzhiev, Ferenc Müller, Didier Casane, Ghislaine Magdelenat, Sylvie Rétaux
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bb2774d9136c4d0e988713db1630a391
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spelling	oai:doaj.org-article:bb2774d9136c4d0e988713db1630a3912021-11-18T07:03:24ZTwo lamprey Hedgehog genes share non-coding regulatory sequences and expression patterns with gnathostome Hedgehogs.1932-620310.1371/journal.pone.0013332https://doaj.org/article/bb2774d9136c4d0e988713db1630a3912010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20967201/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Hedgehog (Hh) genes play major roles in animal development and studies of their evolution, expression and function point to major differences among chordates. Here we focused on Hh genes in lampreys in order to characterize the evolution of Hh signalling at the emergence of vertebrates. Screening of a cosmid library of the river lamprey Lampetra fluviatilis and searching the preliminary genome assembly of the sea lamprey Petromyzon marinus indicate that lampreys have two Hh genes, named Hha and Hhb. Phylogenetic analyses suggest that Hha and Hhb are lamprey-specific paralogs closely related to Sonic/Indian Hh genes. Expression analysis indicates that Hha and Hhb are expressed in a Sonic Hh-like pattern. The two transcripts are expressed in largely overlapping but not identical domains in the lamprey embryonic brain, including a newly-described expression domain in the nasohypophyseal placode. Global alignments of genomic sequences and local alignment with known gnathostome regulatory motifs show that lamprey Hhs share conserved non-coding elements (CNE) with gnathostome Hhs albeit with sequences that have significantly diverged and dispersed. Functional assays using zebrafish embryos demonstrate gnathostome-like midline enhancer activity for CNEs contained in intron2. We conclude that lamprey Hh genes are gnathostome Shh-like in terms of expression and regulation. In addition, they show some lamprey-specific features, including duplication and structural (but not functional) changes in the intronic/regulatory sequences.Shungo KanoJin-Hua XiaoJoana OsórioMarc EkkerYavor HadzhievFerenc MüllerDidier CasaneGhislaine MagdelenatSylvie RétauxPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10, p e13332 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shungo Kano Jin-Hua Xiao Joana Osório Marc Ekker Yavor Hadzhiev Ferenc Müller Didier Casane Ghislaine Magdelenat Sylvie Rétaux Two lamprey Hedgehog genes share non-coding regulatory sequences and expression patterns with gnathostome Hedgehogs.
description	Hedgehog (Hh) genes play major roles in animal development and studies of their evolution, expression and function point to major differences among chordates. Here we focused on Hh genes in lampreys in order to characterize the evolution of Hh signalling at the emergence of vertebrates. Screening of a cosmid library of the river lamprey Lampetra fluviatilis and searching the preliminary genome assembly of the sea lamprey Petromyzon marinus indicate that lampreys have two Hh genes, named Hha and Hhb. Phylogenetic analyses suggest that Hha and Hhb are lamprey-specific paralogs closely related to Sonic/Indian Hh genes. Expression analysis indicates that Hha and Hhb are expressed in a Sonic Hh-like pattern. The two transcripts are expressed in largely overlapping but not identical domains in the lamprey embryonic brain, including a newly-described expression domain in the nasohypophyseal placode. Global alignments of genomic sequences and local alignment with known gnathostome regulatory motifs show that lamprey Hhs share conserved non-coding elements (CNE) with gnathostome Hhs albeit with sequences that have significantly diverged and dispersed. Functional assays using zebrafish embryos demonstrate gnathostome-like midline enhancer activity for CNEs contained in intron2. We conclude that lamprey Hh genes are gnathostome Shh-like in terms of expression and regulation. In addition, they show some lamprey-specific features, including duplication and structural (but not functional) changes in the intronic/regulatory sequences.
format	article
author	Shungo Kano Jin-Hua Xiao Joana Osório Marc Ekker Yavor Hadzhiev Ferenc Müller Didier Casane Ghislaine Magdelenat Sylvie Rétaux
author_facet	Shungo Kano Jin-Hua Xiao Joana Osório Marc Ekker Yavor Hadzhiev Ferenc Müller Didier Casane Ghislaine Magdelenat Sylvie Rétaux
author_sort	Shungo Kano
title	Two lamprey Hedgehog genes share non-coding regulatory sequences and expression patterns with gnathostome Hedgehogs.
title_short	Two lamprey Hedgehog genes share non-coding regulatory sequences and expression patterns with gnathostome Hedgehogs.
title_full	Two lamprey Hedgehog genes share non-coding regulatory sequences and expression patterns with gnathostome Hedgehogs.
title_fullStr	Two lamprey Hedgehog genes share non-coding regulatory sequences and expression patterns with gnathostome Hedgehogs.
title_full_unstemmed	Two lamprey Hedgehog genes share non-coding regulatory sequences and expression patterns with gnathostome Hedgehogs.
title_sort	two lamprey hedgehog genes share non-coding regulatory sequences and expression patterns with gnathostome hedgehogs.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/bb2774d9136c4d0e988713db1630a391
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Two lamprey Hedgehog genes share non-coding regulatory sequences and expression patterns with gnathostome Hedgehogs.

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