The two faces of mast cells in vitiligo pathogenesis

Aim: Previously, we reported increased number of T helper 17 (Th17) cells in vitiligo. However, in our recent study, tryptase and interleukin (IL)17 double positive cells which identified by polyclonal anti-IL17 antibody with specificity for IL17A, B, D, F was observed, but these mast cells cannot b...

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Autores principales:	Ichiro Katayama, Lingli Yang, Aya Takahashi, Fei Yang, Mari Wataya-Kaneda
Formato:	article
Lenguaje:	EN
Publicado:	Open Exploration Publishing Inc. 2021
Materias:	vitiligo rhododendrol-induced leukoderma mast cell tryptase interleukin 17 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/bb2aecab03c042e98034b6788300bf41
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spelling	oai:doaj.org-article:bb2aecab03c042e98034b6788300bf412021-11-24T06:38:07ZThe two faces of mast cells in vitiligo pathogenesis10.37349/ei.2021.000182768-6655https://doaj.org/article/bb2aecab03c042e98034b6788300bf412021-10-01T00:00:00Zhttps://www.explorationpub.com/Journals/ei/Article/100318https://doaj.org/toc/2768-6655Aim: Previously, we reported increased number of T helper 17 (Th17) cells in vitiligo. However, in our recent study, tryptase and interleukin (IL)17 double positive cells which identified by polyclonal anti-IL17 antibody with specificity for IL17A, B, D, F was observed, but these mast cells cannot be stained by monoclonal anti-IL17 antibody with specificity for IL17A. Therefore, this study was aimed to clarify the role of mast cells in induction and progression of vitiligo. Methods: Mast cells were stained with two antibodies against IL17 and one antibody against tryptase by immunofluorescent staining. Furthermore, immunoelectron microscopy (IEM) analyses were conducted using anti-tryptase. In vitro, cultured epidermal keratinocytes were treated with agents which released by mast cells. Expression levels of mRNA were analyzed by real-time polymerase chain reaction (PCR), expression of protein levels was analyzed by western blotting. Results: An increased number of tryptase positive mast cells was observed at the lesional skin of upper dermis in vitiligo and rhododendrol-induced leukoderma (RDIL). These mast cells showed prominent degranulation in vitiligo. Interestingly, the melanosome forming glycoprotein non-metastatic melanoma protein B (GPNMB) is downregulated in the lesional basal keratinocytes in vitiligo and mast cell tryptase contributes to this phenomenon. In addition, small interfering GPNMB RNA (siGPNMB RNA)-introduced keratinocytes increased melanocyte survival through stem cell factor (SCF) production in the melanocyte/keratinocyte co-culture system. Conclusions: Mast cells might be two-faced in vitiligo induction, progression, and recovery through the differential function of histamine and tryptase.Ichiro KatayamaLingli YangAya TakahashiFei YangMari Wataya-KanedaOpen Exploration Publishing Inc.articlevitiligorhododendrol-induced leukodermamast celltryptaseinterleukin 17Immunologic diseases. AllergyRC581-607ENExploration of Immunology, Vol 1, Iss 4, Pp 269-284 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	vitiligo rhododendrol-induced leukoderma mast cell tryptase interleukin 17 Immunologic diseases. Allergy RC581-607
spellingShingle	vitiligo rhododendrol-induced leukoderma mast cell tryptase interleukin 17 Immunologic diseases. Allergy RC581-607 Ichiro Katayama Lingli Yang Aya Takahashi Fei Yang Mari Wataya-Kaneda The two faces of mast cells in vitiligo pathogenesis
description	Aim: Previously, we reported increased number of T helper 17 (Th17) cells in vitiligo. However, in our recent study, tryptase and interleukin (IL)17 double positive cells which identified by polyclonal anti-IL17 antibody with specificity for IL17A, B, D, F was observed, but these mast cells cannot be stained by monoclonal anti-IL17 antibody with specificity for IL17A. Therefore, this study was aimed to clarify the role of mast cells in induction and progression of vitiligo. Methods: Mast cells were stained with two antibodies against IL17 and one antibody against tryptase by immunofluorescent staining. Furthermore, immunoelectron microscopy (IEM) analyses were conducted using anti-tryptase. In vitro, cultured epidermal keratinocytes were treated with agents which released by mast cells. Expression levels of mRNA were analyzed by real-time polymerase chain reaction (PCR), expression of protein levels was analyzed by western blotting. Results: An increased number of tryptase positive mast cells was observed at the lesional skin of upper dermis in vitiligo and rhododendrol-induced leukoderma (RDIL). These mast cells showed prominent degranulation in vitiligo. Interestingly, the melanosome forming glycoprotein non-metastatic melanoma protein B (GPNMB) is downregulated in the lesional basal keratinocytes in vitiligo and mast cell tryptase contributes to this phenomenon. In addition, small interfering GPNMB RNA (siGPNMB RNA)-introduced keratinocytes increased melanocyte survival through stem cell factor (SCF) production in the melanocyte/keratinocyte co-culture system. Conclusions: Mast cells might be two-faced in vitiligo induction, progression, and recovery through the differential function of histamine and tryptase.
format	article
author	Ichiro Katayama Lingli Yang Aya Takahashi Fei Yang Mari Wataya-Kaneda
author_facet	Ichiro Katayama Lingli Yang Aya Takahashi Fei Yang Mari Wataya-Kaneda
author_sort	Ichiro Katayama
title	The two faces of mast cells in vitiligo pathogenesis
title_short	The two faces of mast cells in vitiligo pathogenesis
title_full	The two faces of mast cells in vitiligo pathogenesis
title_fullStr	The two faces of mast cells in vitiligo pathogenesis
title_full_unstemmed	The two faces of mast cells in vitiligo pathogenesis
title_sort	two faces of mast cells in vitiligo pathogenesis
publisher	Open Exploration Publishing Inc.
publishDate	2021
url	https://doaj.org/article/bb2aecab03c042e98034b6788300bf41
work_keys_str_mv	AT ichirokatayama thetwofacesofmastcellsinvitiligopathogenesis AT lingliyang thetwofacesofmastcellsinvitiligopathogenesis AT ayatakahashi thetwofacesofmastcellsinvitiligopathogenesis AT feiyang thetwofacesofmastcellsinvitiligopathogenesis AT mariwatayakaneda thetwofacesofmastcellsinvitiligopathogenesis AT ichirokatayama twofacesofmastcellsinvitiligopathogenesis AT lingliyang twofacesofmastcellsinvitiligopathogenesis AT ayatakahashi twofacesofmastcellsinvitiligopathogenesis AT feiyang twofacesofmastcellsinvitiligopathogenesis AT mariwatayakaneda twofacesofmastcellsinvitiligopathogenesis
_version_	1718415937597603840

The two faces of mast cells in vitiligo pathogenesis

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