Improved phenotyping procedure for evaluating resistance in rice against gall midge (Orseolia oryzae, Wood-Mason)

Abstract Background The rice gall midge (RGM, Orseolia oryzae, Wood-Mason), an important stem-feeding pest worldwide, has caused serious production losses over the past decades. Rice production practices indicate that the most reliable method for managing RGM is the deployment of cultivars that inco...

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Autores principales:	Ling Cheng, Fugang Huang, Zhe Jiang, Baiyi Lu, Xiaohui Zhong, Yongfu Qiu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Rice gall midge (RGM) Chewing insect High-throughput phenotyping method (HTPM) Gene mapping Plant culture SB1-1110 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bb3d597b9ec74697940a016342e83235
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spelling	oai:doaj.org-article:bb3d597b9ec74697940a016342e832352021-12-05T12:07:41ZImproved phenotyping procedure for evaluating resistance in rice against gall midge (Orseolia oryzae, Wood-Mason)10.1186/s13007-021-00823-51746-4811https://doaj.org/article/bb3d597b9ec74697940a016342e832352021-11-01T00:00:00Zhttps://doi.org/10.1186/s13007-021-00823-5https://doaj.org/toc/1746-4811Abstract Background The rice gall midge (RGM, Orseolia oryzae, Wood-Mason), an important stem-feeding pest worldwide, has caused serious production losses over the past decades. Rice production practices indicate that the most reliable method for managing RGM is the deployment of cultivars that incorporate host resistance. However, the conventional phenotypic screening method of rice resistance to RGM suggested by the International Rice Research Institute (IRRI) has been used for approximately 30 years, and only 12 rice varieties/lines (including controls) can be evaluated in one tray. It is not suitable for high-throughput phenotyping of rice germplasm. Moreover, a suitable method to prepare samples for molecular biological studies of rice resistance against RGM is imperative with the rapid development of modern molecular techniques. Results The proper density of seedlings/RGM was determined for four seeding arrangements. A high-throughput phenotyping method (HTPM) for 60 lines/varieties infested with 36 female RGM adults in one tray, as described by method 4–3 (seeded 60 lines/varieties), was developed and verified using mutant screening. Furthermore, one RGM resistance gene flanked by markers 12RM28346 and 12RM28739 on chromosome 12 was simultaneously detected using method 2–2 (seeded 30 lines/varieties in one tray) treated with 24 RGM and analyzed using conventional and simplified grading systems. Genetic analysis of the RGM resistance gene was confirmed using a method identical to that suggested by IRRI. Finally, one bucket with 24 seedlings treated with at least five female RGM adults was efficacious and could offer adequate samples for insect development observation or molecular biological studies. Conclusion A highly efficient and reliable procedure for evaluation of resistance in rice to RGM was developed and improved, and was verified through mutant screening, gene mapping, genetic analysis, and insect growth and development observations.Ling ChengFugang HuangZhe JiangBaiyi LuXiaohui ZhongYongfu QiuBMCarticleRice gall midge (RGM)Chewing insectHigh-throughput phenotyping method (HTPM)Gene mappingPlant cultureSB1-1110Biology (General)QH301-705.5ENPlant Methods, Vol 17, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Rice gall midge (RGM) Chewing insect High-throughput phenotyping method (HTPM) Gene mapping Plant culture SB1-1110 Biology (General) QH301-705.5
spellingShingle	Rice gall midge (RGM) Chewing insect High-throughput phenotyping method (HTPM) Gene mapping Plant culture SB1-1110 Biology (General) QH301-705.5 Ling Cheng Fugang Huang Zhe Jiang Baiyi Lu Xiaohui Zhong Yongfu Qiu Improved phenotyping procedure for evaluating resistance in rice against gall midge (Orseolia oryzae, Wood-Mason)
description	Abstract Background The rice gall midge (RGM, Orseolia oryzae, Wood-Mason), an important stem-feeding pest worldwide, has caused serious production losses over the past decades. Rice production practices indicate that the most reliable method for managing RGM is the deployment of cultivars that incorporate host resistance. However, the conventional phenotypic screening method of rice resistance to RGM suggested by the International Rice Research Institute (IRRI) has been used for approximately 30 years, and only 12 rice varieties/lines (including controls) can be evaluated in one tray. It is not suitable for high-throughput phenotyping of rice germplasm. Moreover, a suitable method to prepare samples for molecular biological studies of rice resistance against RGM is imperative with the rapid development of modern molecular techniques. Results The proper density of seedlings/RGM was determined for four seeding arrangements. A high-throughput phenotyping method (HTPM) for 60 lines/varieties infested with 36 female RGM adults in one tray, as described by method 4–3 (seeded 60 lines/varieties), was developed and verified using mutant screening. Furthermore, one RGM resistance gene flanked by markers 12RM28346 and 12RM28739 on chromosome 12 was simultaneously detected using method 2–2 (seeded 30 lines/varieties in one tray) treated with 24 RGM and analyzed using conventional and simplified grading systems. Genetic analysis of the RGM resistance gene was confirmed using a method identical to that suggested by IRRI. Finally, one bucket with 24 seedlings treated with at least five female RGM adults was efficacious and could offer adequate samples for insect development observation or molecular biological studies. Conclusion A highly efficient and reliable procedure for evaluation of resistance in rice to RGM was developed and improved, and was verified through mutant screening, gene mapping, genetic analysis, and insect growth and development observations.
format	article
author	Ling Cheng Fugang Huang Zhe Jiang Baiyi Lu Xiaohui Zhong Yongfu Qiu
author_facet	Ling Cheng Fugang Huang Zhe Jiang Baiyi Lu Xiaohui Zhong Yongfu Qiu
author_sort	Ling Cheng
title	Improved phenotyping procedure for evaluating resistance in rice against gall midge (Orseolia oryzae, Wood-Mason)
title_short	Improved phenotyping procedure for evaluating resistance in rice against gall midge (Orseolia oryzae, Wood-Mason)
title_full	Improved phenotyping procedure for evaluating resistance in rice against gall midge (Orseolia oryzae, Wood-Mason)
title_fullStr	Improved phenotyping procedure for evaluating resistance in rice against gall midge (Orseolia oryzae, Wood-Mason)
title_full_unstemmed	Improved phenotyping procedure for evaluating resistance in rice against gall midge (Orseolia oryzae, Wood-Mason)
title_sort	improved phenotyping procedure for evaluating resistance in rice against gall midge (orseolia oryzae, wood-mason)
publisher	BMC
publishDate	2021
url	https://doaj.org/article/bb3d597b9ec74697940a016342e83235
work_keys_str_mv	AT lingcheng improvedphenotypingprocedureforevaluatingresistanceinriceagainstgallmidgeorseoliaoryzaewoodmason AT fuganghuang improvedphenotypingprocedureforevaluatingresistanceinriceagainstgallmidgeorseoliaoryzaewoodmason AT zhejiang improvedphenotypingprocedureforevaluatingresistanceinriceagainstgallmidgeorseoliaoryzaewoodmason AT baiyilu improvedphenotypingprocedureforevaluatingresistanceinriceagainstgallmidgeorseoliaoryzaewoodmason AT xiaohuizhong improvedphenotypingprocedureforevaluatingresistanceinriceagainstgallmidgeorseoliaoryzaewoodmason AT yongfuqiu improvedphenotypingprocedureforevaluatingresistanceinriceagainstgallmidgeorseoliaoryzaewoodmason
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Improved phenotyping procedure for evaluating resistance in rice against gall midge (Orseolia oryzae, Wood-Mason)

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