BST2/tetherin inhibits dengue virus release from human hepatoma cells.

<h4>Unlabelled</h4>Type I interferons (IFN) have been shown to play an important role for inhibiting Dengue virus (DENV) infection. Identifying IFN-induced cellular proteins are essential for understanding its mechanisms against DENV. Here we established stable Huh7-derived cell lines ex...

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Autores principales:	Xiao-Ben Pan, Jin-Chao Han, Xu Cong, Lai Wei
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bb474a19d6a04f27b5cbc2b05740bc70
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spelling	oai:doaj.org-article:bb474a19d6a04f27b5cbc2b05740bc702021-11-18T08:06:01ZBST2/tetherin inhibits dengue virus release from human hepatoma cells.1932-620310.1371/journal.pone.0051033https://doaj.org/article/bb474a19d6a04f27b5cbc2b05740bc702012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23236425/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Unlabelled</h4>Type I interferons (IFN) have been shown to play an important role for inhibiting Dengue virus (DENV) infection. Identifying IFN-induced cellular proteins are essential for understanding its mechanisms against DENV. Here we established stable Huh7-derived cell lines expressing the IFN-induced cell membrane protein BST2 (Huh7-BST2) or its variant bearing a V5 tag at the C-terminal (Huh7-BST5CV5). These cell lines were infected with DENV to determine proteins modulating their anti-DENV response. We found that expression of BST2 did not affect the efficiency of DENV infection and intracellular replication. Rather, it significantly reduced the virion yield of the infected cells, particularly at low MOI infection. In addition, BST2 also decreased the foci formation and the size of infectious foci in cultured Huh7 monolayers with media containing methocellulose. The addition of the V5 tag at C-terminal inhibited the GPI modification of BST2 and blocked its shift from endoplasm to cytoplastic membrane. BST2CV5 did not affect DENV infection and foci formation in Huh7 cells but reduced virion yield by 1 log at low MOI infection. Interestingly, intracellular BST2CV5 expression was reduced by high level of DENV production.<h4>Conclusion</h4>Our results imply that BST2 is a functional mediator of the IFN response against DENV infection. BST2 inhibits the release of DENV virions from Huh7 cells and limits viral cell-to-cell transmission. BST2CV5 variant is unable to inhibit DENV release but impairs viral infection in cells.Xiao-Ben PanJin-Chao HanXu CongLai WeiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51033 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiao-Ben Pan Jin-Chao Han Xu Cong Lai Wei BST2/tetherin inhibits dengue virus release from human hepatoma cells.
description	<h4>Unlabelled</h4>Type I interferons (IFN) have been shown to play an important role for inhibiting Dengue virus (DENV) infection. Identifying IFN-induced cellular proteins are essential for understanding its mechanisms against DENV. Here we established stable Huh7-derived cell lines expressing the IFN-induced cell membrane protein BST2 (Huh7-BST2) or its variant bearing a V5 tag at the C-terminal (Huh7-BST5CV5). These cell lines were infected with DENV to determine proteins modulating their anti-DENV response. We found that expression of BST2 did not affect the efficiency of DENV infection and intracellular replication. Rather, it significantly reduced the virion yield of the infected cells, particularly at low MOI infection. In addition, BST2 also decreased the foci formation and the size of infectious foci in cultured Huh7 monolayers with media containing methocellulose. The addition of the V5 tag at C-terminal inhibited the GPI modification of BST2 and blocked its shift from endoplasm to cytoplastic membrane. BST2CV5 did not affect DENV infection and foci formation in Huh7 cells but reduced virion yield by 1 log at low MOI infection. Interestingly, intracellular BST2CV5 expression was reduced by high level of DENV production.<h4>Conclusion</h4>Our results imply that BST2 is a functional mediator of the IFN response against DENV infection. BST2 inhibits the release of DENV virions from Huh7 cells and limits viral cell-to-cell transmission. BST2CV5 variant is unable to inhibit DENV release but impairs viral infection in cells.
format	article
author	Xiao-Ben Pan Jin-Chao Han Xu Cong Lai Wei
author_facet	Xiao-Ben Pan Jin-Chao Han Xu Cong Lai Wei
author_sort	Xiao-Ben Pan
title	BST2/tetherin inhibits dengue virus release from human hepatoma cells.
title_short	BST2/tetherin inhibits dengue virus release from human hepatoma cells.
title_full	BST2/tetherin inhibits dengue virus release from human hepatoma cells.
title_fullStr	BST2/tetherin inhibits dengue virus release from human hepatoma cells.
title_full_unstemmed	BST2/tetherin inhibits dengue virus release from human hepatoma cells.
title_sort	bst2/tetherin inhibits dengue virus release from human hepatoma cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/bb474a19d6a04f27b5cbc2b05740bc70
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BST2/tetherin inhibits dengue virus release from human hepatoma cells.

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