Systematic tissue collection during clinical breast biopsy is feasible, safe and enables high-content translational analyses

Abstract Systematic collection of fresh tissues for research at the time of diagnostic image-guided breast biopsy has the potential to fuel a wide variety of innovative studies. Here we report the initial experience, including safety, feasibility, and laboratory proof-of-principle, with the collecti...

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Autores principales:	Siang-Boon Koh, Brian N. Dontchos, Veerle Bossuyt, Christine Edmonds, Simona Cristea, Nsan Melkonjan, Lindsey Mortensen, Annie Ma, Kassidy Beyerlin, Elyssa Denault, Elizabeth Niehoff, Taghreed Hirz, David B. Sykes, Franziska Michor, Michelle Specht, Constance Lehman, Leif W. Ellisen, Laura M. Spring
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/bb4e9fd066134280a9174b2fe59dc262
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spelling	oai:doaj.org-article:bb4e9fd066134280a9174b2fe59dc2622021-12-02T18:14:07ZSystematic tissue collection during clinical breast biopsy is feasible, safe and enables high-content translational analyses10.1038/s41698-021-00224-w2397-768Xhttps://doaj.org/article/bb4e9fd066134280a9174b2fe59dc2622021-09-01T00:00:00Zhttps://doi.org/10.1038/s41698-021-00224-whttps://doaj.org/toc/2397-768XAbstract Systematic collection of fresh tissues for research at the time of diagnostic image-guided breast biopsy has the potential to fuel a wide variety of innovative studies. Here we report the initial experience, including safety, feasibility, and laboratory proof-of-principle, with the collection and analysis of research specimens obtained via breast core needle biopsy immediately following routine clinical biopsy at a single institution over a 14-month period. Patients underwent one or two additional core biopsies following collection of all necessary clinical specimens. In total, 395 patients were approached and 270 consented to the research study, yielding a 68.4% consent rate. Among consenting patients, 238 lesions were biopsied for research, resulting in 446 research specimens collected. No immediate complications were observed. Representative research core specimens showed high diagnostic concordance with clinical core biopsies. Flow cytometry demonstrated consistent recovery of hundreds to thousands of viable cells per research core. Among a group of HER2 + tumor research specimens, HER2 assessment by flow cytometry correlated highly with immunohistochemistry (IHC) staining, and in addition revealed extensive inter- and intra-tumoral variation in HER2 levels of potential clinical relevance. Suitability for single-cell transcriptomic analysis was demonstrated for a triple-negative tumor core biopsy, revealing substantial cellular diversity in the tumor immune microenvironment, including a prognostically relevant T cell subpopulation. Thus, collection of fresh tissues for research purposes at the time of diagnostic breast biopsy is safe, feasible and efficient, and may provide a high-yield mechanism to generate a rich tissue repository for a wide variety of cross-disciplinary research.Siang-Boon KohBrian N. DontchosVeerle BossuytChristine EdmondsSimona CristeaNsan MelkonjanLindsey MortensenAnnie MaKassidy BeyerlinElyssa DenaultElizabeth NiehoffTaghreed HirzDavid B. SykesFranziska MichorMichelle SpechtConstance LehmanLeif W. EllisenLaura M. SpringNature PortfolioarticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Precision Oncology, Vol 5, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Siang-Boon Koh Brian N. Dontchos Veerle Bossuyt Christine Edmonds Simona Cristea Nsan Melkonjan Lindsey Mortensen Annie Ma Kassidy Beyerlin Elyssa Denault Elizabeth Niehoff Taghreed Hirz David B. Sykes Franziska Michor Michelle Specht Constance Lehman Leif W. Ellisen Laura M. Spring Systematic tissue collection during clinical breast biopsy is feasible, safe and enables high-content translational analyses
description	Abstract Systematic collection of fresh tissues for research at the time of diagnostic image-guided breast biopsy has the potential to fuel a wide variety of innovative studies. Here we report the initial experience, including safety, feasibility, and laboratory proof-of-principle, with the collection and analysis of research specimens obtained via breast core needle biopsy immediately following routine clinical biopsy at a single institution over a 14-month period. Patients underwent one or two additional core biopsies following collection of all necessary clinical specimens. In total, 395 patients were approached and 270 consented to the research study, yielding a 68.4% consent rate. Among consenting patients, 238 lesions were biopsied for research, resulting in 446 research specimens collected. No immediate complications were observed. Representative research core specimens showed high diagnostic concordance with clinical core biopsies. Flow cytometry demonstrated consistent recovery of hundreds to thousands of viable cells per research core. Among a group of HER2 + tumor research specimens, HER2 assessment by flow cytometry correlated highly with immunohistochemistry (IHC) staining, and in addition revealed extensive inter- and intra-tumoral variation in HER2 levels of potential clinical relevance. Suitability for single-cell transcriptomic analysis was demonstrated for a triple-negative tumor core biopsy, revealing substantial cellular diversity in the tumor immune microenvironment, including a prognostically relevant T cell subpopulation. Thus, collection of fresh tissues for research purposes at the time of diagnostic breast biopsy is safe, feasible and efficient, and may provide a high-yield mechanism to generate a rich tissue repository for a wide variety of cross-disciplinary research.
format	article
author	Siang-Boon Koh Brian N. Dontchos Veerle Bossuyt Christine Edmonds Simona Cristea Nsan Melkonjan Lindsey Mortensen Annie Ma Kassidy Beyerlin Elyssa Denault Elizabeth Niehoff Taghreed Hirz David B. Sykes Franziska Michor Michelle Specht Constance Lehman Leif W. Ellisen Laura M. Spring
author_facet	Siang-Boon Koh Brian N. Dontchos Veerle Bossuyt Christine Edmonds Simona Cristea Nsan Melkonjan Lindsey Mortensen Annie Ma Kassidy Beyerlin Elyssa Denault Elizabeth Niehoff Taghreed Hirz David B. Sykes Franziska Michor Michelle Specht Constance Lehman Leif W. Ellisen Laura M. Spring
author_sort	Siang-Boon Koh
title	Systematic tissue collection during clinical breast biopsy is feasible, safe and enables high-content translational analyses
title_short	Systematic tissue collection during clinical breast biopsy is feasible, safe and enables high-content translational analyses
title_full	Systematic tissue collection during clinical breast biopsy is feasible, safe and enables high-content translational analyses
title_fullStr	Systematic tissue collection during clinical breast biopsy is feasible, safe and enables high-content translational analyses
title_full_unstemmed	Systematic tissue collection during clinical breast biopsy is feasible, safe and enables high-content translational analyses
title_sort	systematic tissue collection during clinical breast biopsy is feasible, safe and enables high-content translational analyses
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bb4e9fd066134280a9174b2fe59dc262
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Systematic tissue collection during clinical breast biopsy is feasible, safe and enables high-content translational analyses

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