Wound administration of M2-polarized macrophages does not improve murine cutaneous healing responses.

Macrophages play a crucial role in all stages of cutaneous wound healing responses and dysregulation of macrophage function can result in derailed wound repair. The phenotype of macrophages is influenced by the wound microenvironment and evolves during healing from a more pro-inflammatory (M1) profi...

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Autores principales:	Nadine Jetten, Nadia Roumans, Marion J Gijbels, Andrea Romano, Mark J Post, Menno P J de Winther, Rene R W J van der Hulst, Sofia Xanthoulea
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bb4f07f96e634049827191034300ae28
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spelling	oai:doaj.org-article:bb4f07f96e634049827191034300ae282021-11-25T06:07:00ZWound administration of M2-polarized macrophages does not improve murine cutaneous healing responses.1932-620310.1371/journal.pone.0102994https://doaj.org/article/bb4f07f96e634049827191034300ae282014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25068282/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Macrophages play a crucial role in all stages of cutaneous wound healing responses and dysregulation of macrophage function can result in derailed wound repair. The phenotype of macrophages is influenced by the wound microenvironment and evolves during healing from a more pro-inflammatory (M1) profile in early stages, to a less inflammatory pro-healing (M2) phenotype in later stages of repair. The aim of the current study was to investigate the potential of exogenous administration of M2 macrophages to promote wound healing in an experimental mouse model of cutaneous injury. Bone marrow derived macrophages were stimulated in-vitro with IL-4 or IL-10 to obtain two different subsets of M2-polarized cells, M2a or M2c respectively. Polarized macrophages were injected into full-thickness excisional skin wounds of either C57BL/6 or diabetic db/db mice. Control groups were injected with non-polarized (M0) macrophages or saline. Our data indicate that despite M2 macrophages exhibit an anti-inflammatory phenotype in-vitro, they do not improve wound closure in wild type mice while they delay healing in diabetic mice. Examination of wounds on day 15 post-injury indicated delayed re-epithelialization and persistence of neutrophils in M2 macrophage treated diabetic wounds. Therefore, topical application of ex-vivo generated M2 macrophages is not beneficial and contraindicated for cell therapy of skin wounds.Nadine JettenNadia RoumansMarion J GijbelsAndrea RomanoMark J PostMenno P J de WintherRene R W J van der HulstSofia XanthouleaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e102994 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nadine Jetten Nadia Roumans Marion J Gijbels Andrea Romano Mark J Post Menno P J de Winther Rene R W J van der Hulst Sofia Xanthoulea Wound administration of M2-polarized macrophages does not improve murine cutaneous healing responses.
description	Macrophages play a crucial role in all stages of cutaneous wound healing responses and dysregulation of macrophage function can result in derailed wound repair. The phenotype of macrophages is influenced by the wound microenvironment and evolves during healing from a more pro-inflammatory (M1) profile in early stages, to a less inflammatory pro-healing (M2) phenotype in later stages of repair. The aim of the current study was to investigate the potential of exogenous administration of M2 macrophages to promote wound healing in an experimental mouse model of cutaneous injury. Bone marrow derived macrophages were stimulated in-vitro with IL-4 or IL-10 to obtain two different subsets of M2-polarized cells, M2a or M2c respectively. Polarized macrophages were injected into full-thickness excisional skin wounds of either C57BL/6 or diabetic db/db mice. Control groups were injected with non-polarized (M0) macrophages or saline. Our data indicate that despite M2 macrophages exhibit an anti-inflammatory phenotype in-vitro, they do not improve wound closure in wild type mice while they delay healing in diabetic mice. Examination of wounds on day 15 post-injury indicated delayed re-epithelialization and persistence of neutrophils in M2 macrophage treated diabetic wounds. Therefore, topical application of ex-vivo generated M2 macrophages is not beneficial and contraindicated for cell therapy of skin wounds.
format	article
author	Nadine Jetten Nadia Roumans Marion J Gijbels Andrea Romano Mark J Post Menno P J de Winther Rene R W J van der Hulst Sofia Xanthoulea
author_facet	Nadine Jetten Nadia Roumans Marion J Gijbels Andrea Romano Mark J Post Menno P J de Winther Rene R W J van der Hulst Sofia Xanthoulea
author_sort	Nadine Jetten
title	Wound administration of M2-polarized macrophages does not improve murine cutaneous healing responses.
title_short	Wound administration of M2-polarized macrophages does not improve murine cutaneous healing responses.
title_full	Wound administration of M2-polarized macrophages does not improve murine cutaneous healing responses.
title_fullStr	Wound administration of M2-polarized macrophages does not improve murine cutaneous healing responses.
title_full_unstemmed	Wound administration of M2-polarized macrophages does not improve murine cutaneous healing responses.
title_sort	wound administration of m2-polarized macrophages does not improve murine cutaneous healing responses.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/bb4f07f96e634049827191034300ae28
work_keys_str_mv	AT nadinejetten woundadministrationofm2polarizedmacrophagesdoesnotimprovemurinecutaneoushealingresponses AT nadiaroumans woundadministrationofm2polarizedmacrophagesdoesnotimprovemurinecutaneoushealingresponses AT marionjgijbels woundadministrationofm2polarizedmacrophagesdoesnotimprovemurinecutaneoushealingresponses AT andrearomano woundadministrationofm2polarizedmacrophagesdoesnotimprovemurinecutaneoushealingresponses AT markjpost woundadministrationofm2polarizedmacrophagesdoesnotimprovemurinecutaneoushealingresponses AT mennopjdewinther woundadministrationofm2polarizedmacrophagesdoesnotimprovemurinecutaneoushealingresponses AT renerwjvanderhulst woundadministrationofm2polarizedmacrophagesdoesnotimprovemurinecutaneoushealingresponses AT sofiaxanthoulea woundadministrationofm2polarizedmacrophagesdoesnotimprovemurinecutaneoushealingresponses
_version_	1718414186712662016

Wound administration of M2-polarized macrophages does not improve murine cutaneous healing responses.

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