Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope

While the application of molecular biological techniques based upon fluorescent probes has rapidly expanded over recent decades, the equipment cost of fluorescent microscopy has largely prevented its adoption in the college and high school classroom. We offer a simple solution to this problem by des...

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Autores principales:	G. A. Babbitt, C. A. Hanzlik, K. N. Busse
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bb4f83327de8481abf6dc762b1b00f55
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spelling	oai:doaj.org-article:bb4f83327de8481abf6dc762b1b00f552021-11-15T15:03:50ZObserving Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope10.1128/jmbe.v14i1.5301935-78851935-7877https://doaj.org/article/bb4f83327de8481abf6dc762b1b00f552013-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v14i1.530https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885While the application of molecular biological techniques based upon fluorescent probes has rapidly expanded over recent decades, the equipment cost of fluorescent microscopy has largely prevented its adoption in the college and high school classroom. We offer a simple solution to this problem by describing in detail how to build with simple tools, a fluorescent microscope using a common brand of colored LED flashlights and second-hand components of vintage Nikon microscopes. This extremely low cost solution is qualitatively compared to an expensive modern Zeiss system.G. A. BabbittC. A. HanzlikK. N. BusseAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 14, Iss 1, Pp 121-124 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 G. A. Babbitt C. A. Hanzlik K. N. Busse Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope
description	While the application of molecular biological techniques based upon fluorescent probes has rapidly expanded over recent decades, the equipment cost of fluorescent microscopy has largely prevented its adoption in the college and high school classroom. We offer a simple solution to this problem by describing in detail how to build with simple tools, a fluorescent microscope using a common brand of colored LED flashlights and second-hand components of vintage Nikon microscopes. This extremely low cost solution is qualitatively compared to an expensive modern Zeiss system.
format	article
author	G. A. Babbitt C. A. Hanzlik K. N. Busse
author_facet	G. A. Babbitt C. A. Hanzlik K. N. Busse
author_sort	G. A. Babbitt
title	Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope
title_short	Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope
title_full	Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope
title_fullStr	Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope
title_full_unstemmed	Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope
title_sort	observing fluorescent probes in living cells using a low-cost led flashlight retrofitted to a common vintage light microscope
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/bb4f83327de8481abf6dc762b1b00f55
work_keys_str_mv	AT gababbitt observingfluorescentprobesinlivingcellsusingalowcostledflashlightretrofittedtoacommonvintagelightmicroscope AT cahanzlik observingfluorescentprobesinlivingcellsusingalowcostledflashlightretrofittedtoacommonvintagelightmicroscope AT knbusse observingfluorescentprobesinlivingcellsusingalowcostledflashlightretrofittedtoacommonvintagelightmicroscope
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Observing Fluorescent Probes in Living Cells using a Low-Cost LED Flashlight Retrofitted to a Common Vintage Light Microscope

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