Singapore Grouper Iridovirus Disturbed Glycerophospholipids Homeostasis: Cytosolic Phospholipase A2 Was Essential for Virus Replication

Singapore grouper iridovirus (SGIV), belonging to genus <i>Ranavirus</i>, family <i>Iridoviridae</i>, causes great economic losses in the aquaculture industry. Previous studies demonstrated the lipid composition of intracellular unenveloped viruses, but the changes in host-ce...

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Autores principales:	Na Ni, Jiaying Zheng, Wenji Wang, Linyong Zhi, Qiwei Qin, Youhua Huang, Xiaohong Huang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cytosolic phospholipase A2 SGIV glycerophospholipids cyclooxygenase (COX) 5-Lipoxygenase (5-LOX) Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/bb588b89c1364d459c2f6fc4833348ea
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spelling	oai:doaj.org-article:bb588b89c1364d459c2f6fc4833348ea2021-11-25T17:58:12ZSingapore Grouper Iridovirus Disturbed Glycerophospholipids Homeostasis: Cytosolic Phospholipase A2 Was Essential for Virus Replication10.3390/ijms2222125971422-00671661-6596https://doaj.org/article/bb588b89c1364d459c2f6fc4833348ea2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12597https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Singapore grouper iridovirus (SGIV), belonging to genus <i>Ranavirus</i>, family <i>Iridoviridae</i>, causes great economic losses in the aquaculture industry. Previous studies demonstrated the lipid composition of intracellular unenveloped viruses, but the changes in host-cell glyceophospholipids components and the roles of key enzymes during SGIV infection still remain largely unknown. Here, the whole cell lipidomic profiling during SGIV infection was analyzed using UPLC-Q-TOF-MS/MS. The lipidomic data showed that glycerophospholipids (GPs), including phosphatidylcholine (PC), phosphatidylserine (PS), glycerophosphoinositols (PI) and fatty acids (FAs) were significantly elevated in SGIV-infected cells, indicating that SGIV infection disturbed GPs homeostasis, and then affected the metabolism of FAs, especially arachidonic acid (AA). The roles of key enzymes, such as cytosolic phospholipase A2 (cPLA2), 5-Lipoxygenase (5-LOX), and cyclooxygenase (COX) in SGIV infection were further investigated using the corresponding specific inhibitors. The inhibition of cPLA2 by AACOCF3 decreased SGIV replication, suggesting that cPLA2 might play important roles in the process of SGIV infection. Consistent with this result, the ectopic expression of EccPLA2α or knockdown significantly enhanced or suppressed viral replication in vitro, respectively. In addition, the inhibition of both 5-LOX and COX significantly suppressed SGIV replication, indicating that AA metabolism was essential for SGIV infection. Taken together, our results demonstrated for the first time that SGIV infection in vitro disturbed GPs homeostasis and cPLA2 exerted crucial roles in SGIV replication.Na NiJiaying ZhengWenji WangLinyong ZhiQiwei QinYouhua HuangXiaohong HuangMDPI AGarticlecytosolic phospholipase A2SGIVglycerophospholipidscyclooxygenase (COX)5-Lipoxygenase (5-LOX)Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12597, p 12597 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cytosolic phospholipase A2 SGIV glycerophospholipids cyclooxygenase (COX) 5-Lipoxygenase (5-LOX) Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	cytosolic phospholipase A2 SGIV glycerophospholipids cyclooxygenase (COX) 5-Lipoxygenase (5-LOX) Biology (General) QH301-705.5 Chemistry QD1-999 Na Ni Jiaying Zheng Wenji Wang Linyong Zhi Qiwei Qin Youhua Huang Xiaohong Huang Singapore Grouper Iridovirus Disturbed Glycerophospholipids Homeostasis: Cytosolic Phospholipase A2 Was Essential for Virus Replication
description	Singapore grouper iridovirus (SGIV), belonging to genus <i>Ranavirus</i>, family <i>Iridoviridae</i>, causes great economic losses in the aquaculture industry. Previous studies demonstrated the lipid composition of intracellular unenveloped viruses, but the changes in host-cell glyceophospholipids components and the roles of key enzymes during SGIV infection still remain largely unknown. Here, the whole cell lipidomic profiling during SGIV infection was analyzed using UPLC-Q-TOF-MS/MS. The lipidomic data showed that glycerophospholipids (GPs), including phosphatidylcholine (PC), phosphatidylserine (PS), glycerophosphoinositols (PI) and fatty acids (FAs) were significantly elevated in SGIV-infected cells, indicating that SGIV infection disturbed GPs homeostasis, and then affected the metabolism of FAs, especially arachidonic acid (AA). The roles of key enzymes, such as cytosolic phospholipase A2 (cPLA2), 5-Lipoxygenase (5-LOX), and cyclooxygenase (COX) in SGIV infection were further investigated using the corresponding specific inhibitors. The inhibition of cPLA2 by AACOCF3 decreased SGIV replication, suggesting that cPLA2 might play important roles in the process of SGIV infection. Consistent with this result, the ectopic expression of EccPLA2α or knockdown significantly enhanced or suppressed viral replication in vitro, respectively. In addition, the inhibition of both 5-LOX and COX significantly suppressed SGIV replication, indicating that AA metabolism was essential for SGIV infection. Taken together, our results demonstrated for the first time that SGIV infection in vitro disturbed GPs homeostasis and cPLA2 exerted crucial roles in SGIV replication.
format	article
author	Na Ni Jiaying Zheng Wenji Wang Linyong Zhi Qiwei Qin Youhua Huang Xiaohong Huang
author_facet	Na Ni Jiaying Zheng Wenji Wang Linyong Zhi Qiwei Qin Youhua Huang Xiaohong Huang
author_sort	Na Ni
title	Singapore Grouper Iridovirus Disturbed Glycerophospholipids Homeostasis: Cytosolic Phospholipase A2 Was Essential for Virus Replication
title_short	Singapore Grouper Iridovirus Disturbed Glycerophospholipids Homeostasis: Cytosolic Phospholipase A2 Was Essential for Virus Replication
title_full	Singapore Grouper Iridovirus Disturbed Glycerophospholipids Homeostasis: Cytosolic Phospholipase A2 Was Essential for Virus Replication
title_fullStr	Singapore Grouper Iridovirus Disturbed Glycerophospholipids Homeostasis: Cytosolic Phospholipase A2 Was Essential for Virus Replication
title_full_unstemmed	Singapore Grouper Iridovirus Disturbed Glycerophospholipids Homeostasis: Cytosolic Phospholipase A2 Was Essential for Virus Replication
title_sort	singapore grouper iridovirus disturbed glycerophospholipids homeostasis: cytosolic phospholipase a2 was essential for virus replication
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bb588b89c1364d459c2f6fc4833348ea
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Singapore Grouper Iridovirus Disturbed Glycerophospholipids Homeostasis: Cytosolic Phospholipase A2 Was Essential for Virus Replication

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