Anti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response

Hypertension is accompanied by the over-activation of macrophages. Diuretics administered alone or in combination with hypotensive drugs may have immunomodulatory effects. Thus, the influence of tested drugs on mouse macrophage-mediated humoral immunity was investigated. Mice were treated intraperit...

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Autores principales:	Paweł Bryniarski, Katarzyna Nazimek, Janusz Marcinkiewicz
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antihypertensive therapy diuretics humoral immunity hypotensive drugs macrophages Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/bb5cc3840cca41e6954aa5fade16265a
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spelling	oai:doaj.org-article:bb5cc3840cca41e6954aa5fade16265a2021-11-11T16:50:51ZAnti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response10.3390/ijms2221113741422-00671661-6596https://doaj.org/article/bb5cc3840cca41e6954aa5fade16265a2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11374https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Hypertension is accompanied by the over-activation of macrophages. Diuretics administered alone or in combination with hypotensive drugs may have immunomodulatory effects. Thus, the influence of tested drugs on mouse macrophage-mediated humoral immunity was investigated. Mice were treated intraperitoneally with captopril (5 mg/kg) with or without hydrochlorothiazide (10 mg/kg) or furosemide (5 mg/kg) by 8 days. Mineral oil-induced peritoneal macrophages were harvested to assess the generation of cytokines in ELISA, and the expression of surface markers was analyzed cytometrically. Macrophages were also pulsed with sheep red blood cells (SRBC) and transferred to naive mice for evaluation of their ability to induce a humoral immune response. Tested drugs increase the expression of surface markers important for the antigen phagocytosis and presentation. SRBC-pulsed macrophages from mice treated with captopril combined with diuretics increased the secretion of antigen-specific antibodies by recipient B cells, while macrophages of mice treated with hydrochlorothiazide or furosemide with captopril increased the number of antigen-specific B cells. Tested drugs alter the macrophage secretory profile in favor of anti-inflammatory cytokines. Our results showed that diuretics with or without captopril modulate the humoral response by affecting the function of macrophages, which has significant translational potential in assessing the safety of antihypertensive therapy.Paweł BryniarskiKatarzyna NazimekJanusz MarcinkiewiczMDPI AGarticleantihypertensive therapydiureticshumoral immunityhypotensive drugsmacrophagesBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11374, p 11374 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antihypertensive therapy diuretics humoral immunity hypotensive drugs macrophages Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	antihypertensive therapy diuretics humoral immunity hypotensive drugs macrophages Biology (General) QH301-705.5 Chemistry QD1-999 Paweł Bryniarski Katarzyna Nazimek Janusz Marcinkiewicz Anti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response
description	Hypertension is accompanied by the over-activation of macrophages. Diuretics administered alone or in combination with hypotensive drugs may have immunomodulatory effects. Thus, the influence of tested drugs on mouse macrophage-mediated humoral immunity was investigated. Mice were treated intraperitoneally with captopril (5 mg/kg) with or without hydrochlorothiazide (10 mg/kg) or furosemide (5 mg/kg) by 8 days. Mineral oil-induced peritoneal macrophages were harvested to assess the generation of cytokines in ELISA, and the expression of surface markers was analyzed cytometrically. Macrophages were also pulsed with sheep red blood cells (SRBC) and transferred to naive mice for evaluation of their ability to induce a humoral immune response. Tested drugs increase the expression of surface markers important for the antigen phagocytosis and presentation. SRBC-pulsed macrophages from mice treated with captopril combined with diuretics increased the secretion of antigen-specific antibodies by recipient B cells, while macrophages of mice treated with hydrochlorothiazide or furosemide with captopril increased the number of antigen-specific B cells. Tested drugs alter the macrophage secretory profile in favor of anti-inflammatory cytokines. Our results showed that diuretics with or without captopril modulate the humoral response by affecting the function of macrophages, which has significant translational potential in assessing the safety of antihypertensive therapy.
format	article
author	Paweł Bryniarski Katarzyna Nazimek Janusz Marcinkiewicz
author_facet	Paweł Bryniarski Katarzyna Nazimek Janusz Marcinkiewicz
author_sort	Paweł Bryniarski
title	Anti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response
title_short	Anti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response
title_full	Anti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response
title_fullStr	Anti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response
title_full_unstemmed	Anti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response
title_sort	anti-inflammatory activities of captopril and diuretics on macrophage activity in mouse humoral immune response
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bb5cc3840cca41e6954aa5fade16265a
work_keys_str_mv	AT pawełbryniarski antiinflammatoryactivitiesofcaptoprilanddiureticsonmacrophageactivityinmousehumoralimmuneresponse AT katarzynanazimek antiinflammatoryactivitiesofcaptoprilanddiureticsonmacrophageactivityinmousehumoralimmuneresponse AT januszmarcinkiewicz antiinflammatoryactivitiesofcaptoprilanddiureticsonmacrophageactivityinmousehumoralimmuneresponse
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Anti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response

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