Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells

Abstract The ability of isolated neural stem cells (NSCs) to proliferate as neurospheres is indicative of their competence as stem cells, and depends critically on the polycomb group (PcG) member Bmi1: knockdown of Bmi1 results in defective proliferation and self-renewal of isolated NSCs, whereas ov...

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Autores principales:	Mythily Ganapathi, Nathan C. Boles, Carol Charniga, Steven Lotz, Melissa Campbell, Sally Temple, Randall H. Morse
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bb6451d396854ca396639407b1055802
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spelling	oai:doaj.org-article:bb6451d396854ca396639407b10558022021-12-02T11:41:23ZEffect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells10.1038/s41598-018-25921-82045-2322https://doaj.org/article/bb6451d396854ca396639407b10558022018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25921-8https://doaj.org/toc/2045-2322Abstract The ability of isolated neural stem cells (NSCs) to proliferate as neurospheres is indicative of their competence as stem cells, and depends critically on the polycomb group (PcG) member Bmi1: knockdown of Bmi1 results in defective proliferation and self-renewal of isolated NSCs, whereas overexpression of Bmi1 enhances these properties. Here we report genome-wide changes in gene expression in embryonic and adult NSCs (eNSCs and aNSCs) caused by overexpression of Bmi1. We find that genes whose expression is altered by perturbations in Bmi1 levels in NSCs are mostly distinct from those affected in other multipotent stem/progenitor cells, such as those from liver and lung, aside from a small core of common targets that is enriched for genes associated with cell migration and mobility. We also show that genes differing in expression between prospectively isolated quiescent and activated NSCs are not affected by Bmi1 overexpression. In contrast, a comparison of genes showing altered expression upon Bmi1 overexpression in eNSCs and in aNSCs reveals considerable overlap, in spite of their different provenances in the brain and their differing developmental programs.Mythily GanapathiNathan C. BolesCarol CharnigaSteven LotzMelissa CampbellSally TempleRandall H. MorseNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mythily Ganapathi Nathan C. Boles Carol Charniga Steven Lotz Melissa Campbell Sally Temple Randall H. Morse Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
description	Abstract The ability of isolated neural stem cells (NSCs) to proliferate as neurospheres is indicative of their competence as stem cells, and depends critically on the polycomb group (PcG) member Bmi1: knockdown of Bmi1 results in defective proliferation and self-renewal of isolated NSCs, whereas overexpression of Bmi1 enhances these properties. Here we report genome-wide changes in gene expression in embryonic and adult NSCs (eNSCs and aNSCs) caused by overexpression of Bmi1. We find that genes whose expression is altered by perturbations in Bmi1 levels in NSCs are mostly distinct from those affected in other multipotent stem/progenitor cells, such as those from liver and lung, aside from a small core of common targets that is enriched for genes associated with cell migration and mobility. We also show that genes differing in expression between prospectively isolated quiescent and activated NSCs are not affected by Bmi1 overexpression. In contrast, a comparison of genes showing altered expression upon Bmi1 overexpression in eNSCs and in aNSCs reveals considerable overlap, in spite of their different provenances in the brain and their differing developmental programs.
format	article
author	Mythily Ganapathi Nathan C. Boles Carol Charniga Steven Lotz Melissa Campbell Sally Temple Randall H. Morse
author_facet	Mythily Ganapathi Nathan C. Boles Carol Charniga Steven Lotz Melissa Campbell Sally Temple Randall H. Morse
author_sort	Mythily Ganapathi
title	Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
title_short	Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
title_full	Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
title_fullStr	Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
title_full_unstemmed	Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
title_sort	effect of bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/bb6451d396854ca396639407b1055802
work_keys_str_mv	AT mythilyganapathi effectofbmi1overexpressionongeneexpressioninadultandembryonicmurineneuralstemcells AT nathancboles effectofbmi1overexpressionongeneexpressioninadultandembryonicmurineneuralstemcells AT carolcharniga effectofbmi1overexpressionongeneexpressioninadultandembryonicmurineneuralstemcells AT stevenlotz effectofbmi1overexpressionongeneexpressioninadultandembryonicmurineneuralstemcells AT melissacampbell effectofbmi1overexpressionongeneexpressioninadultandembryonicmurineneuralstemcells AT sallytemple effectofbmi1overexpressionongeneexpressioninadultandembryonicmurineneuralstemcells AT randallhmorse effectofbmi1overexpressionongeneexpressioninadultandembryonicmurineneuralstemcells
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Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells

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