Isolation and Characterization of Bacteria that Produce Polyhydroxybutyrate Depolymerases

Isolation of environmental bacterial strains with diverse metabolic properties such as antibiotic production, sulfide oxidation, and the ability to use different nutrient sources is a common undergraduate lab experience. This article describes a useful protocol for the detection and isolation of bac...

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Autores principales:	Emily A. Egusa, Daniel J. Edwards, MyLo L. Thao, Larry L. Kirk, Larry F. Hanne
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bb6bc98a58404358a1802c1f9987c843
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spelling	oai:doaj.org-article:bb6bc98a58404358a1802c1f9987c8432021-11-15T15:04:14ZIsolation and Characterization of Bacteria that Produce Polyhydroxybutyrate Depolymerases10.1128/jmbe.v19i3.16821935-78851935-7877https://doaj.org/article/bb6bc98a58404358a1802c1f9987c8432018-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v19i3.1682https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Isolation of environmental bacterial strains with diverse metabolic properties such as antibiotic production, sulfide oxidation, and the ability to use different nutrient sources is a common undergraduate lab experience. This article describes a useful protocol for the detection and isolation of bacteria that can degrade the common carbon storage biopolymer poly (3-hydroxybutyrate) (PHB). The procedure utilizes a low nutrient media supplemented with insoluble PHB that allows for the easy, visible detection of strains that produce extracellular PHB degrading enzymes. This method can be used to determine the percent of culturable bacteria from various environments that can degrade PHB. Further, PHB degrading exoenzyme activity can be monitored from pure cultures by: (1) Growing cells in broth, (2) inducing with PHB, and (3) measuring activity of supernatant with a UV/Vis spectrophotometer. The enzymatic breakdown of PHB presents an opportunity to expose students to the concept of using biodegradable plastics as a solution to the global plastic waste problem.Emily A. EgusaDaniel J. EdwardsMyLo L. ThaoLarry L. KirkLarry F. HanneAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 19, Iss 3 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Emily A. Egusa Daniel J. Edwards MyLo L. Thao Larry L. Kirk Larry F. Hanne Isolation and Characterization of Bacteria that Produce Polyhydroxybutyrate Depolymerases
description	Isolation of environmental bacterial strains with diverse metabolic properties such as antibiotic production, sulfide oxidation, and the ability to use different nutrient sources is a common undergraduate lab experience. This article describes a useful protocol for the detection and isolation of bacteria that can degrade the common carbon storage biopolymer poly (3-hydroxybutyrate) (PHB). The procedure utilizes a low nutrient media supplemented with insoluble PHB that allows for the easy, visible detection of strains that produce extracellular PHB degrading enzymes. This method can be used to determine the percent of culturable bacteria from various environments that can degrade PHB. Further, PHB degrading exoenzyme activity can be monitored from pure cultures by: (1) Growing cells in broth, (2) inducing with PHB, and (3) measuring activity of supernatant with a UV/Vis spectrophotometer. The enzymatic breakdown of PHB presents an opportunity to expose students to the concept of using biodegradable plastics as a solution to the global plastic waste problem.
format	article
author	Emily A. Egusa Daniel J. Edwards MyLo L. Thao Larry L. Kirk Larry F. Hanne
author_facet	Emily A. Egusa Daniel J. Edwards MyLo L. Thao Larry L. Kirk Larry F. Hanne
author_sort	Emily A. Egusa
title	Isolation and Characterization of Bacteria that Produce Polyhydroxybutyrate Depolymerases
title_short	Isolation and Characterization of Bacteria that Produce Polyhydroxybutyrate Depolymerases
title_full	Isolation and Characterization of Bacteria that Produce Polyhydroxybutyrate Depolymerases
title_fullStr	Isolation and Characterization of Bacteria that Produce Polyhydroxybutyrate Depolymerases
title_full_unstemmed	Isolation and Characterization of Bacteria that Produce Polyhydroxybutyrate Depolymerases
title_sort	isolation and characterization of bacteria that produce polyhydroxybutyrate depolymerases
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/bb6bc98a58404358a1802c1f9987c843
work_keys_str_mv	AT emilyaegusa isolationandcharacterizationofbacteriathatproducepolyhydroxybutyratedepolymerases AT danieljedwards isolationandcharacterizationofbacteriathatproducepolyhydroxybutyratedepolymerases AT mylolthao isolationandcharacterizationofbacteriathatproducepolyhydroxybutyratedepolymerases AT larrylkirk isolationandcharacterizationofbacteriathatproducepolyhydroxybutyratedepolymerases AT larryfhanne isolationandcharacterizationofbacteriathatproducepolyhydroxybutyratedepolymerases
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Isolation and Characterization of Bacteria that Produce Polyhydroxybutyrate Depolymerases

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