Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries
Existing Förster Resonance Energy Transfer (FRET) biosensors are often limited in their sensitivity. Here the authors report FRET-seq which they use to identify Fyn and ZAP70 kinase biosensors with enhanced performance, and use them to image T-cell activation and screen drugs.
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Nature Portfolio
2021
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oai:doaj.org-article:bb6ff210871349e99294dd89352b85062021-12-02T16:45:48ZIntegration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries10.1038/s41467-021-25323-x2041-1723https://doaj.org/article/bb6ff210871349e99294dd89352b85062021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25323-xhttps://doaj.org/toc/2041-1723Existing Förster Resonance Energy Transfer (FRET) biosensors are often limited in their sensitivity. Here the authors report FRET-seq which they use to identify Fyn and ZAP70 kinase biosensors with enhanced performance, and use them to image T-cell activation and screen drugs.Longwei LiuPraopim LimsakulXianhui MengYan HuangReed E. S. HarrisonTse-Shun HuangYiwen ShiYiyan YuKrit CharupanitSheng ZhongShaoying LuJin ZhangShu ChienJie SunYingxiao WangNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-16 (2021) |
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DOAJ |
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DOAJ |
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EN |
| topic |
Science Q |
| spellingShingle |
Science Q Longwei Liu Praopim Limsakul Xianhui Meng Yan Huang Reed E. S. Harrison Tse-Shun Huang Yiwen Shi Yiyan Yu Krit Charupanit Sheng Zhong Shaoying Lu Jin Zhang Shu Chien Jie Sun Yingxiao Wang Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries |
| description |
Existing Förster Resonance Energy Transfer (FRET) biosensors are often limited in their sensitivity. Here the authors report FRET-seq which they use to identify Fyn and ZAP70 kinase biosensors with enhanced performance, and use them to image T-cell activation and screen drugs. |
| format |
article |
| author |
Longwei Liu Praopim Limsakul Xianhui Meng Yan Huang Reed E. S. Harrison Tse-Shun Huang Yiwen Shi Yiyan Yu Krit Charupanit Sheng Zhong Shaoying Lu Jin Zhang Shu Chien Jie Sun Yingxiao Wang |
| author_facet |
Longwei Liu Praopim Limsakul Xianhui Meng Yan Huang Reed E. S. Harrison Tse-Shun Huang Yiwen Shi Yiyan Yu Krit Charupanit Sheng Zhong Shaoying Lu Jin Zhang Shu Chien Jie Sun Yingxiao Wang |
| author_sort |
Longwei Liu |
| title |
Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries |
| title_short |
Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries |
| title_full |
Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries |
| title_fullStr |
Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries |
| title_full_unstemmed |
Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries |
| title_sort |
integration of fret and sequencing to engineer kinase biosensors from mammalian cell libraries |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/bb6ff210871349e99294dd89352b8506 |
| work_keys_str_mv |
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