A new genetic linkage map of the zygomycete fungus Phycomyces blakesleeanus.

Phycomyces blakesleeanus is a member of the subphylum Mucoromycotina. A genetic map was constructed from 121 progeny of a cross between two wild type isolates of P. blakesleeanus with 134 markers. The markers were mostly PCR-RFLPs. Markers were located on 46 scaffolds of the genome sequence, coverin...

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Autores principales:	Suman Chaudhary, Silvia Polaino, Viplendra P S Shakya, Alexander Idnurm
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bb74c92c01df4a249be037161d641b17
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spelling	oai:doaj.org-article:bb74c92c01df4a249be037161d641b172021-11-18T07:53:24ZA new genetic linkage map of the zygomycete fungus Phycomyces blakesleeanus.1932-620310.1371/journal.pone.0058931https://doaj.org/article/bb74c92c01df4a249be037161d641b172013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23516579/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Phycomyces blakesleeanus is a member of the subphylum Mucoromycotina. A genetic map was constructed from 121 progeny of a cross between two wild type isolates of P. blakesleeanus with 134 markers. The markers were mostly PCR-RFLPs. Markers were located on 46 scaffolds of the genome sequence, covering more than 97% of the genome. Analysis of the alleles in the progeny revealed nine or 12 linkage groups, depending on the log of the odds (LOD) score, across 1583.4 cM at LOD 5. The linkage groups were overlaid on previous mapping data from crosses between mutants, aided by new identification of the mutations in primary metabolism mutant strains. The molecular marker map, the phenotype map and the genome sequence are overall congruent, with some exceptions. The new genetic map provides a genome-wide estimate for recombination, with the average of 33.2 kb per cM. This frequency is one piece of evidence for meiosis during zygospore development in Mucoromycotina species. At the same time as meiosis, transmission of non-recombinant chromosomes is also evident in the mating process in Phycomyces. The new map provides scaffold ordering for the genome sequence and a platform upon which to identify the genes in mutants that are affected in traits of interest, such as carotene biosynthesis, phototropism or gravitropism, using positional cloning.Suman ChaudharySilvia PolainoViplendra P S ShakyaAlexander IdnurmPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58931 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Suman Chaudhary Silvia Polaino Viplendra P S Shakya Alexander Idnurm A new genetic linkage map of the zygomycete fungus Phycomyces blakesleeanus.
description	Phycomyces blakesleeanus is a member of the subphylum Mucoromycotina. A genetic map was constructed from 121 progeny of a cross between two wild type isolates of P. blakesleeanus with 134 markers. The markers were mostly PCR-RFLPs. Markers were located on 46 scaffolds of the genome sequence, covering more than 97% of the genome. Analysis of the alleles in the progeny revealed nine or 12 linkage groups, depending on the log of the odds (LOD) score, across 1583.4 cM at LOD 5. The linkage groups were overlaid on previous mapping data from crosses between mutants, aided by new identification of the mutations in primary metabolism mutant strains. The molecular marker map, the phenotype map and the genome sequence are overall congruent, with some exceptions. The new genetic map provides a genome-wide estimate for recombination, with the average of 33.2 kb per cM. This frequency is one piece of evidence for meiosis during zygospore development in Mucoromycotina species. At the same time as meiosis, transmission of non-recombinant chromosomes is also evident in the mating process in Phycomyces. The new map provides scaffold ordering for the genome sequence and a platform upon which to identify the genes in mutants that are affected in traits of interest, such as carotene biosynthesis, phototropism or gravitropism, using positional cloning.
format	article
author	Suman Chaudhary Silvia Polaino Viplendra P S Shakya Alexander Idnurm
author_facet	Suman Chaudhary Silvia Polaino Viplendra P S Shakya Alexander Idnurm
author_sort	Suman Chaudhary
title	A new genetic linkage map of the zygomycete fungus Phycomyces blakesleeanus.
title_short	A new genetic linkage map of the zygomycete fungus Phycomyces blakesleeanus.
title_full	A new genetic linkage map of the zygomycete fungus Phycomyces blakesleeanus.
title_fullStr	A new genetic linkage map of the zygomycete fungus Phycomyces blakesleeanus.
title_full_unstemmed	A new genetic linkage map of the zygomycete fungus Phycomyces blakesleeanus.
title_sort	new genetic linkage map of the zygomycete fungus phycomyces blakesleeanus.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/bb74c92c01df4a249be037161d641b17
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A new genetic linkage map of the zygomycete fungus Phycomyces blakesleeanus.

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