A method for detecting long non-coding RNAs with tiled RNA expression microarrays.
Long non-coding ribonucleic acids (lncRNAs) have been proposed as biomarkers in prostate cancer. This paper proposes a selection method which uses data from tiled microarrays to identify relatively long regions of moderate expression independent of the microarray platform and probe design. The metho...
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Public Library of Science (PLoS)
2014
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oai:doaj.org-article:bb755d99fe6e462c9c016cdb49384e6c2021-11-18T08:15:22ZA method for detecting long non-coding RNAs with tiled RNA expression microarrays.1932-620310.1371/journal.pone.0099899https://doaj.org/article/bb755d99fe6e462c9c016cdb49384e6c2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24937006/?tool=EBIhttps://doaj.org/toc/1932-6203Long non-coding ribonucleic acids (lncRNAs) have been proposed as biomarkers in prostate cancer. This paper proposes a selection method which uses data from tiled microarrays to identify relatively long regions of moderate expression independent of the microarray platform and probe design. The method is used to search for candidate long non-coding ribonucleic acids (lncRNAs) at locus 8q24 and is run on three independent experiments which all use samples from prostate cancer patients. The robustness of the method is tested by utilizing repeated copies of tiled probes. The method shows high consistency between experiments that used the same samples, but different probe layout. There also is statistically significant consistency when comparing experiments with different samples. The method selected the long non-coding ribonucleic acid PCNCR1 in all three experiments.Sigrun Helga LundDaniel Fannar GudbjartssonThorunn RafnarAsgeir SigurdssonSigurjon Axel GudjonssonJulius GudmundssonKari StefanssonGunnar StefanssonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e99899 (2014) |
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Medicine R Science Q |
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Medicine R Science Q Sigrun Helga Lund Daniel Fannar Gudbjartsson Thorunn Rafnar Asgeir Sigurdsson Sigurjon Axel Gudjonsson Julius Gudmundsson Kari Stefansson Gunnar Stefansson A method for detecting long non-coding RNAs with tiled RNA expression microarrays. |
| description |
Long non-coding ribonucleic acids (lncRNAs) have been proposed as biomarkers in prostate cancer. This paper proposes a selection method which uses data from tiled microarrays to identify relatively long regions of moderate expression independent of the microarray platform and probe design. The method is used to search for candidate long non-coding ribonucleic acids (lncRNAs) at locus 8q24 and is run on three independent experiments which all use samples from prostate cancer patients. The robustness of the method is tested by utilizing repeated copies of tiled probes. The method shows high consistency between experiments that used the same samples, but different probe layout. There also is statistically significant consistency when comparing experiments with different samples. The method selected the long non-coding ribonucleic acid PCNCR1 in all three experiments. |
| format |
article |
| author |
Sigrun Helga Lund Daniel Fannar Gudbjartsson Thorunn Rafnar Asgeir Sigurdsson Sigurjon Axel Gudjonsson Julius Gudmundsson Kari Stefansson Gunnar Stefansson |
| author_facet |
Sigrun Helga Lund Daniel Fannar Gudbjartsson Thorunn Rafnar Asgeir Sigurdsson Sigurjon Axel Gudjonsson Julius Gudmundsson Kari Stefansson Gunnar Stefansson |
| author_sort |
Sigrun Helga Lund |
| title |
A method for detecting long non-coding RNAs with tiled RNA expression microarrays. |
| title_short |
A method for detecting long non-coding RNAs with tiled RNA expression microarrays. |
| title_full |
A method for detecting long non-coding RNAs with tiled RNA expression microarrays. |
| title_fullStr |
A method for detecting long non-coding RNAs with tiled RNA expression microarrays. |
| title_full_unstemmed |
A method for detecting long non-coding RNAs with tiled RNA expression microarrays. |
| title_sort |
method for detecting long non-coding rnas with tiled rna expression microarrays. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/bb755d99fe6e462c9c016cdb49384e6c |
| work_keys_str_mv |
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