Plant Regeneration and <i>In Vitro</i> Growth Performance of Male-Sterile Somatic Plantlets of Sugi (Japanese Cedar, <i>Cryptomeria japonica</i>) Derived from Different Embryogenic Cell Lines
With the spread of pollinosis caused by sugi (Japanese cedar, <i>Cryptomeria japonica</i>) pollen, the use of pollen-free somatic seedlings of sugi is expected in Japan. However, there is a lack of knowledge on the relationship between the abilities during somatic embryogenesis, initial...
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| Auteurs principaux: | , , , , |
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| Format: | article |
| Langue: | EN |
| Publié: |
MDPI AG
2021
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| Accès en ligne: | https://doaj.org/article/bb7dedfb14e54774b9e5c777756c461d |
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| Résumé: | With the spread of pollinosis caused by sugi (Japanese cedar, <i>Cryptomeria japonica</i>) pollen, the use of pollen-free somatic seedlings of sugi is expected in Japan. However, there is a lack of knowledge on the relationship between the abilities during somatic embryogenesis, initial <i>in vitro</i> growth traits, and subsequent growth of somatic seedlings. In the present study, we provide the first basic information on somatic embryo maturation efficiency, somatic embryo germination, and plantlet conversion frequencies, as well as on <i>in vitro</i> growth performance of pollen-free somatic plantlets derived from different embryogenic cell lines (ECLs). Somatic embryo maturation efficiency varied from 34 to 514 cotyledonary embryos per plate and the average for the 19 ECLs tested was 244 embryos per plate. Subsequently, the overall average rates of somatic embryo germination and conversion among ECLs were 87.8% and 85.3%, respectively. The results of <i>in vitro</i> growth performance of pollen-free somatic plantlets showed significant differences in growth rate among ECLs. |
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