Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae

Pegah Shakib1,2, Sobhan Ghafourian1, Mohammad Reza Zolfaghary2, Reza Hushmandfar3, Reza Ranjbar4, Nourkhoda Sadeghifard11Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam; 2Department of Microbiology, Qom Branch, Islamic Azad University of Qom, Qom; 3Faculty of Veterin...

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Autores principales:	Shakib P, Ghafourian S, Zolfaghary MR, Hushmandfar R, Ranjbar R, Sadeghifard N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bb83c984b56f4537a27034a7b04b629c
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spelling	oai:doaj.org-article:bb83c984b56f4537a27034a7b04b629c2021-12-02T05:02:28ZPrevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae1177-54751177-5491https://doaj.org/article/bb83c984b56f4537a27034a7b04b629c2011-12-01T00:00:00Zhttp://www.dovepress.com/prevalence-of-ompk35-and-ompk36-porin-expression-in-beta-lactamase-and-a8943https://doaj.org/toc/1177-5475https://doaj.org/toc/1177-5491Pegah Shakib1,2, Sobhan Ghafourian1, Mohammad Reza Zolfaghary2, Reza Hushmandfar3, Reza Ranjbar4, Nourkhoda Sadeghifard11Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam; 2Department of Microbiology, Qom Branch, Islamic Azad University of Qom, Qom; 3Faculty of Veterinary Medicine, Ilam University, Ilam; 4Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranBackground: The aims of this study were to confirm the presence of OmpK35 and OmpK36 in extended-spectrum beta-lactamase-producing and nonextended-spectrum beta-lactamase-producing Klebsiella pneumoniae and to determine the relationship between porin expression and resistance to third-generation cephalosporins.Methods: Fifty-two K. pneumoniae isolates were obtained and analyzed for extended-spectrum beta-lactamase and for OmpK35 and OmpK36.Results: Twenty-two (42.3%) isolates of K. pneumoniae were extended-spectrum beta-lactamase producers. The OmpK35 profile in K. pneumoniae producing extended-spectrum beta-lactamase showed the presence of porin protein in ceftazidime-sensitive K. pneumoniae (six isolates), and the OmpK36 profile in K. pneumoniae producing extended-spectrum beta-lactamase revealed isolates sensitive to cefotaxime (n = 8) and ceftriaxone (n = 6). All nonextended-spectrum beta-lactamase-producing K. pneumoniae showed the presence of OmpK35 and OmpK36 porin proteins.Conclusion: The presence of OmpK35 is mostly related to ceftazidime susceptibility and less to cefotaxime and ceftriaxone susceptibility, while OmpK36 expression is seen more often in cefotaxime-sensitive isolates. OmpK35 and OmpK36 indicate nonextended-spectrum beta-lactamase producing strains, and their presence is important when selecting an antimicrobial agent.Keywords: Klebsiella pneumoniae, extended-spectrum beta-lactamase producing strains, porin expressionShakib PGhafourian SZolfaghary MRHushmandfar RRanjbar RSadeghifard NDove Medical PressarticleMedicine (General)R5-920ENBiologics: Targets & Therapy, Vol 2012, Iss default, Pp 1-4 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Shakib P Ghafourian S Zolfaghary MR Hushmandfar R Ranjbar R Sadeghifard N Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae
description	Pegah Shakib1,2, Sobhan Ghafourian1, Mohammad Reza Zolfaghary2, Reza Hushmandfar3, Reza Ranjbar4, Nourkhoda Sadeghifard11Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam; 2Department of Microbiology, Qom Branch, Islamic Azad University of Qom, Qom; 3Faculty of Veterinary Medicine, Ilam University, Ilam; 4Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranBackground: The aims of this study were to confirm the presence of OmpK35 and OmpK36 in extended-spectrum beta-lactamase-producing and nonextended-spectrum beta-lactamase-producing Klebsiella pneumoniae and to determine the relationship between porin expression and resistance to third-generation cephalosporins.Methods: Fifty-two K. pneumoniae isolates were obtained and analyzed for extended-spectrum beta-lactamase and for OmpK35 and OmpK36.Results: Twenty-two (42.3%) isolates of K. pneumoniae were extended-spectrum beta-lactamase producers. The OmpK35 profile in K. pneumoniae producing extended-spectrum beta-lactamase showed the presence of porin protein in ceftazidime-sensitive K. pneumoniae (six isolates), and the OmpK36 profile in K. pneumoniae producing extended-spectrum beta-lactamase revealed isolates sensitive to cefotaxime (n = 8) and ceftriaxone (n = 6). All nonextended-spectrum beta-lactamase-producing K. pneumoniae showed the presence of OmpK35 and OmpK36 porin proteins.Conclusion: The presence of OmpK35 is mostly related to ceftazidime susceptibility and less to cefotaxime and ceftriaxone susceptibility, while OmpK36 expression is seen more often in cefotaxime-sensitive isolates. OmpK35 and OmpK36 indicate nonextended-spectrum beta-lactamase producing strains, and their presence is important when selecting an antimicrobial agent.Keywords: Klebsiella pneumoniae, extended-spectrum beta-lactamase producing strains, porin expression
format	article
author	Shakib P Ghafourian S Zolfaghary MR Hushmandfar R Ranjbar R Sadeghifard N
author_facet	Shakib P Ghafourian S Zolfaghary MR Hushmandfar R Ranjbar R Sadeghifard N
author_sort	Shakib P
title	Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae
title_short	Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae
title_full	Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae
title_fullStr	Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae
title_full_unstemmed	Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae
title_sort	prevalence of ompk35 and ompk36 porin expression in beta-lactamase and non-beta-lactamase-producing klebsiella pneumoniae
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/bb83c984b56f4537a27034a7b04b629c
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Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-beta-lactamase-producing Klebsiella pneumoniae

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