Proteome analysis of human sebaceous follicle infundibula extracted from healthy and acne-affected skin.

Acne vulgaris is a very common disease of the pilosebaceous unit of the human skin. The pathological processes of acne are not fully understood. To gain further insight sebaceous follicular casts were extracted from 18 healthy and 20 acne-affected individuals by cyanoacrylate-gel biopsies and furthe...

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Autores principales:	Malene Bek-Thomsen, Hans B Lomholt, Carsten Scavenius, Jan J Enghild, Holger Brüggemann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bb8636b1dc664f7f94d5e7a5e344e0bd
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spelling	oai:doaj.org-article:bb8636b1dc664f7f94d5e7a5e344e0bd2021-11-25T06:00:01ZProteome analysis of human sebaceous follicle infundibula extracted from healthy and acne-affected skin.1932-620310.1371/journal.pone.0107908https://doaj.org/article/bb8636b1dc664f7f94d5e7a5e344e0bd2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107908https://doaj.org/toc/1932-6203Acne vulgaris is a very common disease of the pilosebaceous unit of the human skin. The pathological processes of acne are not fully understood. To gain further insight sebaceous follicular casts were extracted from 18 healthy and 20 acne-affected individuals by cyanoacrylate-gel biopsies and further processed for mass spectrometry analysis, aiming at a proteomic analysis of the sebaceous follicular casts. Human as well as bacterial proteins were identified. Human proteins enriched in acne and normal samples were detected, respectively. Normal follicular casts are enriched in proteins such as prohibitins and peroxiredoxins which are involved in the protection from various stresses, including reactive oxygen species. By contrast, follicular casts extracted from acne-affected skin contained proteins involved in inflammation, wound healing and tissue remodeling. Among the most distinguishing proteins were myeloperoxidase, lactotransferrin, neutrophil elastase inhibitor and surprisingly, vimentin. The most significant biological process among all acne-enriched proteins was 'response to a bacterium'. Identified bacterial proteins were exclusively from Propionibacterium acnes. The most abundant P. acnes proteins were surface-exposed dermatan sulphate adhesins, CAMP factors, and a so far uncharacterized lipase in follicular casts extracted from normal as well as acne-affected skin. This is a first proteomic study that identified human proteins together with proteins of the skin microbiota in sebaceous follicular casts.Malene Bek-ThomsenHans B LomholtCarsten ScaveniusJan J EnghildHolger BrüggemannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107908 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Malene Bek-Thomsen Hans B Lomholt Carsten Scavenius Jan J Enghild Holger Brüggemann Proteome analysis of human sebaceous follicle infundibula extracted from healthy and acne-affected skin.
description	Acne vulgaris is a very common disease of the pilosebaceous unit of the human skin. The pathological processes of acne are not fully understood. To gain further insight sebaceous follicular casts were extracted from 18 healthy and 20 acne-affected individuals by cyanoacrylate-gel biopsies and further processed for mass spectrometry analysis, aiming at a proteomic analysis of the sebaceous follicular casts. Human as well as bacterial proteins were identified. Human proteins enriched in acne and normal samples were detected, respectively. Normal follicular casts are enriched in proteins such as prohibitins and peroxiredoxins which are involved in the protection from various stresses, including reactive oxygen species. By contrast, follicular casts extracted from acne-affected skin contained proteins involved in inflammation, wound healing and tissue remodeling. Among the most distinguishing proteins were myeloperoxidase, lactotransferrin, neutrophil elastase inhibitor and surprisingly, vimentin. The most significant biological process among all acne-enriched proteins was 'response to a bacterium'. Identified bacterial proteins were exclusively from Propionibacterium acnes. The most abundant P. acnes proteins were surface-exposed dermatan sulphate adhesins, CAMP factors, and a so far uncharacterized lipase in follicular casts extracted from normal as well as acne-affected skin. This is a first proteomic study that identified human proteins together with proteins of the skin microbiota in sebaceous follicular casts.
format	article
author	Malene Bek-Thomsen Hans B Lomholt Carsten Scavenius Jan J Enghild Holger Brüggemann
author_facet	Malene Bek-Thomsen Hans B Lomholt Carsten Scavenius Jan J Enghild Holger Brüggemann
author_sort	Malene Bek-Thomsen
title	Proteome analysis of human sebaceous follicle infundibula extracted from healthy and acne-affected skin.
title_short	Proteome analysis of human sebaceous follicle infundibula extracted from healthy and acne-affected skin.
title_full	Proteome analysis of human sebaceous follicle infundibula extracted from healthy and acne-affected skin.
title_fullStr	Proteome analysis of human sebaceous follicle infundibula extracted from healthy and acne-affected skin.
title_full_unstemmed	Proteome analysis of human sebaceous follicle infundibula extracted from healthy and acne-affected skin.
title_sort	proteome analysis of human sebaceous follicle infundibula extracted from healthy and acne-affected skin.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/bb8636b1dc664f7f94d5e7a5e344e0bd
work_keys_str_mv	AT malenebekthomsen proteomeanalysisofhumansebaceousfollicleinfundibulaextractedfromhealthyandacneaffectedskin AT hansblomholt proteomeanalysisofhumansebaceousfollicleinfundibulaextractedfromhealthyandacneaffectedskin AT carstenscavenius proteomeanalysisofhumansebaceousfollicleinfundibulaextractedfromhealthyandacneaffectedskin AT janjenghild proteomeanalysisofhumansebaceousfollicleinfundibulaextractedfromhealthyandacneaffectedskin AT holgerbruggemann proteomeanalysisofhumansebaceousfollicleinfundibulaextractedfromhealthyandacneaffectedskin
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Proteome analysis of human sebaceous follicle infundibula extracted from healthy and acne-affected skin.

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