Genetic Analysis and Pathogenic Characterization of Alternaria tenuissima Induced Fruit Rot of Bitter Gourd

Abstract. Iftikhar S, Anwar W, Akhter A, Ali S, Khan HAA, Khurshid M, Haider MS. 2021. Genetic analysis and pathogenic characterization of Alternaria tenuissima induced fruit rot of bitter gourd. Biodiversitas 22: 617-625. Bitter gourd (Momordica charantia Linn.), belongs to Cucurbitaceae family, is...

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Autores principales:	SEHRISH IFTIKHAR, WAHEED ANWAR, Adnan Akhter, SAJID ALI, HAFIZ AZHAR ALI KHAN, MUHAMMAD KHURSHID, MUHAMMAD SALEEM HAIDER
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	alternaria rot, emerging disease, microscopic examination, momordica charantia, phylogenetics, phytopathogenic Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bb8b393626c3434885a2443ba3040539
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spelling	oai:doaj.org-article:bb8b393626c3434885a2443ba30405392021-11-22T00:54:56ZGenetic Analysis and Pathogenic Characterization of Alternaria tenuissima Induced Fruit Rot of Bitter Gourd1412-033X2085-472210.13057/biodiv/d220213https://doaj.org/article/bb8b393626c3434885a2443ba30405392021-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7262https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Iftikhar S, Anwar W, Akhter A, Ali S, Khan HAA, Khurshid M, Haider MS. 2021. Genetic analysis and pathogenic characterization of Alternaria tenuissima induced fruit rot of bitter gourd. Biodiversitas 22: 617-625. Bitter gourd (Momordica charantia Linn.), belongs to Cucurbitaceae family, is widely cultivated in areas with warm climate. In 2017, fruits of bitter gourd-bearing rot symptoms were observed in the Punjab province of Pakistan. The disease-causing fungal isolate was collected from the diseased fruits on potato dextrose agar (PDA). Microscopic examination revealed short conidiophores arose singly, measuring 79.8- 158.5 ?m long and 3.94-7.89 ?m thick. The size of conidia varied from 25.7 to 46.45 ?m and 8.55-14.39 ?m in length and width respectively, which were characteristics of Alternaria spp. To confirm the identity and molecular characterization of the isolate, the internal transcribed spacer (ITS) region, translation elongation factor 1 alpha (TEF1-?), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and RNA polymerase II large subunit 2 (RPB2) genes were amplified. The sequence analysis of amplicons and phylogenetic studies specified the homology of isolated Alternaria spp. with the previously reported A. tenuissima in GeneBank. The pathogenicity tests conducted on the fruits of bitter gourd confirmed the disease development with typical Alternaria induced rot symptoms, thus satisfied Koch's postulate. To our knowledge, this is the first record of A. tenuissima causing fruit rot of bitter gourd in Pakistan.SEHRISH IFTIKHARWAHEED ANWARAdnan AkhterSAJID ALIHAFIZ AZHAR ALI KHANMUHAMMAD KHURSHIDMUHAMMAD SALEEM HAIDERMBI & UNS Soloarticlealternaria rot, emerging disease, microscopic examination, momordica charantia, phylogenetics, phytopathogenicBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	alternaria rot, emerging disease, microscopic examination, momordica charantia, phylogenetics, phytopathogenic Biology (General) QH301-705.5
spellingShingle	alternaria rot, emerging disease, microscopic examination, momordica charantia, phylogenetics, phytopathogenic Biology (General) QH301-705.5 SEHRISH IFTIKHAR WAHEED ANWAR Adnan Akhter SAJID ALI HAFIZ AZHAR ALI KHAN MUHAMMAD KHURSHID MUHAMMAD SALEEM HAIDER Genetic Analysis and Pathogenic Characterization of Alternaria tenuissima Induced Fruit Rot of Bitter Gourd
description	Abstract. Iftikhar S, Anwar W, Akhter A, Ali S, Khan HAA, Khurshid M, Haider MS. 2021. Genetic analysis and pathogenic characterization of Alternaria tenuissima induced fruit rot of bitter gourd. Biodiversitas 22: 617-625. Bitter gourd (Momordica charantia Linn.), belongs to Cucurbitaceae family, is widely cultivated in areas with warm climate. In 2017, fruits of bitter gourd-bearing rot symptoms were observed in the Punjab province of Pakistan. The disease-causing fungal isolate was collected from the diseased fruits on potato dextrose agar (PDA). Microscopic examination revealed short conidiophores arose singly, measuring 79.8- 158.5 ?m long and 3.94-7.89 ?m thick. The size of conidia varied from 25.7 to 46.45 ?m and 8.55-14.39 ?m in length and width respectively, which were characteristics of Alternaria spp. To confirm the identity and molecular characterization of the isolate, the internal transcribed spacer (ITS) region, translation elongation factor 1 alpha (TEF1-?), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and RNA polymerase II large subunit 2 (RPB2) genes were amplified. The sequence analysis of amplicons and phylogenetic studies specified the homology of isolated Alternaria spp. with the previously reported A. tenuissima in GeneBank. The pathogenicity tests conducted on the fruits of bitter gourd confirmed the disease development with typical Alternaria induced rot symptoms, thus satisfied Koch's postulate. To our knowledge, this is the first record of A. tenuissima causing fruit rot of bitter gourd in Pakistan.
format	article
author	SEHRISH IFTIKHAR WAHEED ANWAR Adnan Akhter SAJID ALI HAFIZ AZHAR ALI KHAN MUHAMMAD KHURSHID MUHAMMAD SALEEM HAIDER
author_facet	SEHRISH IFTIKHAR WAHEED ANWAR Adnan Akhter SAJID ALI HAFIZ AZHAR ALI KHAN MUHAMMAD KHURSHID MUHAMMAD SALEEM HAIDER
author_sort	SEHRISH IFTIKHAR
title	Genetic Analysis and Pathogenic Characterization of Alternaria tenuissima Induced Fruit Rot of Bitter Gourd
title_short	Genetic Analysis and Pathogenic Characterization of Alternaria tenuissima Induced Fruit Rot of Bitter Gourd
title_full	Genetic Analysis and Pathogenic Characterization of Alternaria tenuissima Induced Fruit Rot of Bitter Gourd
title_fullStr	Genetic Analysis and Pathogenic Characterization of Alternaria tenuissima Induced Fruit Rot of Bitter Gourd
title_full_unstemmed	Genetic Analysis and Pathogenic Characterization of Alternaria tenuissima Induced Fruit Rot of Bitter Gourd
title_sort	genetic analysis and pathogenic characterization of alternaria tenuissima induced fruit rot of bitter gourd
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/bb8b393626c3434885a2443ba3040539
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Genetic Analysis and Pathogenic Characterization of Alternaria tenuissima Induced Fruit Rot of Bitter Gourd

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