Generation of germline-competent rat induced pluripotent stem cells.

<h4>Background</h4>Recent progress in rat pluripotent stem cell technology has been remarkable. Particularly salient is the demonstration that embryonic stem cells (ESCs) in the rat (rESCs) can contribute to germline transmission, permitting generation of gene-modified rats as is now don...

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Autores principales:	Sanae Hamanaka, Tomoyuki Yamaguchi, Toshihiro Kobayashi, Megumi Kato-Itoh, Satoshi Yamazaki, Hideyuki Sato, Ayumi Umino, Yukiko Wakiyama, Mami Arai, Makoto Sanbo, Masumi Hirabayashi, Hiromitsu Nakauchi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bb97da4ca9d24a609a6133340f236fb3
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spelling	oai:doaj.org-article:bb97da4ca9d24a609a6133340f236fb32021-11-18T06:50:12ZGeneration of germline-competent rat induced pluripotent stem cells.1932-620310.1371/journal.pone.0022008https://doaj.org/article/bb97da4ca9d24a609a6133340f236fb32011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21789202/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Recent progress in rat pluripotent stem cell technology has been remarkable. Particularly salient is the demonstration that embryonic stem cells (ESCs) in the rat (rESCs) can contribute to germline transmission, permitting generation of gene-modified rats as is now done using mouse ESCs (mESCs) or mouse induced pluripotent stem cells (iPSCs; miPSCs). However, determinations of whether rat iPSCs (riPSCs) can contribute to germ cells are not published. Here we report the germline competency of riPSCs.<h4>Methodology/principal findings</h4>We generated riPSCs by transducing three mouse reprogramming factors (Oct3/4, Klf4, and Sox2) into rat somatic cells, followed by culture in the presence of exogenous rat leukemia inhibitory factor (rLIF) and small molecules that specifically inhibit GSK3, MEK, and FGF receptor tyrosine kinases. We found that, like rESCs, our riPSCs can contribute to germline transmission. Furthermore we found, by immunostaining of testis from mouse-rat interspecific chimeras with antibody against mouse vasa homolog, that riPSCs can contribute to embryonic development with chimera formation in mice (rat-mouse interspecific chimeras) and to interspecific germlines.<h4>Conclusions/significance</h4>Our data clearly demonstrate that using only three reprogramming factors (Oct3/4, Klf4, and Sox2) rat somatic cells can be reprogrammed into a ground state. Our generated riPSCs exhibited germline transmission in either rat-rat intraspecific or mouse-rat interspecific chimeras.Sanae HamanakaTomoyuki YamaguchiToshihiro KobayashiMegumi Kato-ItohSatoshi YamazakiHideyuki SatoAyumi UminoYukiko WakiyamaMami AraiMakoto SanboMasumi HirabayashiHiromitsu NakauchiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22008 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sanae Hamanaka Tomoyuki Yamaguchi Toshihiro Kobayashi Megumi Kato-Itoh Satoshi Yamazaki Hideyuki Sato Ayumi Umino Yukiko Wakiyama Mami Arai Makoto Sanbo Masumi Hirabayashi Hiromitsu Nakauchi Generation of germline-competent rat induced pluripotent stem cells.
description	<h4>Background</h4>Recent progress in rat pluripotent stem cell technology has been remarkable. Particularly salient is the demonstration that embryonic stem cells (ESCs) in the rat (rESCs) can contribute to germline transmission, permitting generation of gene-modified rats as is now done using mouse ESCs (mESCs) or mouse induced pluripotent stem cells (iPSCs; miPSCs). However, determinations of whether rat iPSCs (riPSCs) can contribute to germ cells are not published. Here we report the germline competency of riPSCs.<h4>Methodology/principal findings</h4>We generated riPSCs by transducing three mouse reprogramming factors (Oct3/4, Klf4, and Sox2) into rat somatic cells, followed by culture in the presence of exogenous rat leukemia inhibitory factor (rLIF) and small molecules that specifically inhibit GSK3, MEK, and FGF receptor tyrosine kinases. We found that, like rESCs, our riPSCs can contribute to germline transmission. Furthermore we found, by immunostaining of testis from mouse-rat interspecific chimeras with antibody against mouse vasa homolog, that riPSCs can contribute to embryonic development with chimera formation in mice (rat-mouse interspecific chimeras) and to interspecific germlines.<h4>Conclusions/significance</h4>Our data clearly demonstrate that using only three reprogramming factors (Oct3/4, Klf4, and Sox2) rat somatic cells can be reprogrammed into a ground state. Our generated riPSCs exhibited germline transmission in either rat-rat intraspecific or mouse-rat interspecific chimeras.
format	article
author	Sanae Hamanaka Tomoyuki Yamaguchi Toshihiro Kobayashi Megumi Kato-Itoh Satoshi Yamazaki Hideyuki Sato Ayumi Umino Yukiko Wakiyama Mami Arai Makoto Sanbo Masumi Hirabayashi Hiromitsu Nakauchi
author_facet	Sanae Hamanaka Tomoyuki Yamaguchi Toshihiro Kobayashi Megumi Kato-Itoh Satoshi Yamazaki Hideyuki Sato Ayumi Umino Yukiko Wakiyama Mami Arai Makoto Sanbo Masumi Hirabayashi Hiromitsu Nakauchi
author_sort	Sanae Hamanaka
title	Generation of germline-competent rat induced pluripotent stem cells.
title_short	Generation of germline-competent rat induced pluripotent stem cells.
title_full	Generation of germline-competent rat induced pluripotent stem cells.
title_fullStr	Generation of germline-competent rat induced pluripotent stem cells.
title_full_unstemmed	Generation of germline-competent rat induced pluripotent stem cells.
title_sort	generation of germline-competent rat induced pluripotent stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/bb97da4ca9d24a609a6133340f236fb3
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Generation of germline-competent rat induced pluripotent stem cells.

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