A Comprehensive Study of the Genus Sanguisorba (Rosaceae) Based on the Floral Micromorphology, Palynology, and Plastome Analysis

Sanguisorba, commonly known as burnet, is a genus in the family Rosaceae native to the temperate regions of the Northern hemisphere. Five of its thirty species are distributed in Korea: Sanguisorba officinalis, S. stipulata, S. hakusa...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Inkyu Park, Junho Song, Sungyu Yang, Goya Choi, Byeongcheol Moon
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Sanguisorba</i> micromorphology pollen plastid genome DNA barcode phylogenic analysis Genetics QH426-470
Acceso en línea:	https://doaj.org/article/bb97ff580108465780adc763de58de3f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bb97ff580108465780adc763de58de3f
record_format	dspace
spelling	oai:doaj.org-article:bb97ff580108465780adc763de58de3f2021-11-25T17:41:40ZA Comprehensive Study of the Genus <i>Sanguisorba</i> (Rosaceae) Based on the Floral Micromorphology, Palynology, and Plastome Analysis10.3390/genes121117642073-4425https://doaj.org/article/bb97ff580108465780adc763de58de3f2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1764https://doaj.org/toc/2073-4425<i>Sanguisorba</i>, commonly known as burnet, is a genus in the family Rosaceae native to the temperate regions of the Northern hemisphere. Five of its thirty species are distributed in Korea: <i>Sanguisorba officinalis</i>, <i>S. stipulata</i>, <i>S. hakusanensis</i>, <i>S. longifolia</i>, and <i>S. tenuifolia</i>. <i>S. officinalis</i> has been designated as a medicinal remedy in the Chinese and Korean Herbal Pharmacopeias. Despite being a valuable medicinal resource, the morphological and genomic information, as well as the genetic characteristics of <i>Sanguisorba</i>, are still elusive. Therefore, we carried out the first comprehensive study on the floral micromorphology, palynology, and complete chloroplast (cp) genome of the <i>Sanguisorba</i> species. The outer sepal waxes and hypanthium characters showed diagnostic value, despite a similar floral micromorphology across different species. All the studied <i>Sanguisorba</i> pollen were small to medium, oblate to prolate-spheroidal, and their exine ornamentation was microechinate. The orbicules, which are possibly synapomorphic, were consistently absent in this genus. Additionally, the cp genomes of <i>S. officinalis</i>, <i>S. stipulata</i>, and <i>S. hakusanensis</i> have been completely sequenced. The comparative analysis of the reported <i>Sanguisorba</i> cp genomes revealed local divergence regions. The nucleotide diversity of <i>trnH-psbA</i> and <i>rps2-rpoC2</i>, referred to as hotspot regions, revealed the highest pi values in six <i>Sanguisorba</i>. The <i>ndhG</i> indicated positive selection pressures as a species-specific variation in <i>S. filiformis</i>. The <i>S. stipulata</i> and <i>S. tenuifolia</i> species had <i>psbK</i> genes at the selected pressures. We developed new DNA barcodes that distinguish the typical <i>S. officinalis</i> and <i>S. officinalis</i> var. <i>longifolia</i>, important herbal medicinal plants, from other similar <i>Sanguisorba</i> species with species-specific distinctive markers. The phylogenetic trees showed the positions of the reported <i>Sanguisorba</i> species; <i>S. officinalis</i>, <i>S. tenuifolia</i>, and <i>S. stipulata</i> showed the nearest genetic distance. The results of our comprehensive study on micromorphology, pollen chemistry, cp genome analysis, and the development of species identification markers can provide valuable information for future studies on <i>S. officinalis</i>, including those highlighting it as an important medicinal resource.Inkyu ParkJunho SongSungyu YangGoya ChoiByeongcheol MoonMDPI AGarticle<i>Sanguisorba</i>micromorphologypollenplastid genomeDNA barcodephylogenic analysisGeneticsQH426-470ENGenes, Vol 12, Iss 1764, p 1764 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Sanguisorba</i> micromorphology pollen plastid genome DNA barcode phylogenic analysis Genetics QH426-470
spellingShingle	<i>Sanguisorba</i> micromorphology pollen plastid genome DNA barcode phylogenic analysis Genetics QH426-470 Inkyu Park Junho Song Sungyu Yang Goya Choi Byeongcheol Moon A Comprehensive Study of the Genus <i>Sanguisorba</i> (Rosaceae) Based on the Floral Micromorphology, Palynology, and Plastome Analysis
description	<i>Sanguisorba</i>, commonly known as burnet, is a genus in the family Rosaceae native to the temperate regions of the Northern hemisphere. Five of its thirty species are distributed in Korea: <i>Sanguisorba officinalis</i>, <i>S. stipulata</i>, <i>S. hakusanensis</i>, <i>S. longifolia</i>, and <i>S. tenuifolia</i>. <i>S. officinalis</i> has been designated as a medicinal remedy in the Chinese and Korean Herbal Pharmacopeias. Despite being a valuable medicinal resource, the morphological and genomic information, as well as the genetic characteristics of <i>Sanguisorba</i>, are still elusive. Therefore, we carried out the first comprehensive study on the floral micromorphology, palynology, and complete chloroplast (cp) genome of the <i>Sanguisorba</i> species. The outer sepal waxes and hypanthium characters showed diagnostic value, despite a similar floral micromorphology across different species. All the studied <i>Sanguisorba</i> pollen were small to medium, oblate to prolate-spheroidal, and their exine ornamentation was microechinate. The orbicules, which are possibly synapomorphic, were consistently absent in this genus. Additionally, the cp genomes of <i>S. officinalis</i>, <i>S. stipulata</i>, and <i>S. hakusanensis</i> have been completely sequenced. The comparative analysis of the reported <i>Sanguisorba</i> cp genomes revealed local divergence regions. The nucleotide diversity of <i>trnH-psbA</i> and <i>rps2-rpoC2</i>, referred to as hotspot regions, revealed the highest pi values in six <i>Sanguisorba</i>. The <i>ndhG</i> indicated positive selection pressures as a species-specific variation in <i>S. filiformis</i>. The <i>S. stipulata</i> and <i>S. tenuifolia</i> species had <i>psbK</i> genes at the selected pressures. We developed new DNA barcodes that distinguish the typical <i>S. officinalis</i> and <i>S. officinalis</i> var. <i>longifolia</i>, important herbal medicinal plants, from other similar <i>Sanguisorba</i> species with species-specific distinctive markers. The phylogenetic trees showed the positions of the reported <i>Sanguisorba</i> species; <i>S. officinalis</i>, <i>S. tenuifolia</i>, and <i>S. stipulata</i> showed the nearest genetic distance. The results of our comprehensive study on micromorphology, pollen chemistry, cp genome analysis, and the development of species identification markers can provide valuable information for future studies on <i>S. officinalis</i>, including those highlighting it as an important medicinal resource.
format	article
author	Inkyu Park Junho Song Sungyu Yang Goya Choi Byeongcheol Moon
author_facet	Inkyu Park Junho Song Sungyu Yang Goya Choi Byeongcheol Moon
author_sort	Inkyu Park
title	A Comprehensive Study of the Genus <i>Sanguisorba</i> (Rosaceae) Based on the Floral Micromorphology, Palynology, and Plastome Analysis
title_short	A Comprehensive Study of the Genus <i>Sanguisorba</i> (Rosaceae) Based on the Floral Micromorphology, Palynology, and Plastome Analysis
title_full	A Comprehensive Study of the Genus <i>Sanguisorba</i> (Rosaceae) Based on the Floral Micromorphology, Palynology, and Plastome Analysis
title_fullStr	A Comprehensive Study of the Genus <i>Sanguisorba</i> (Rosaceae) Based on the Floral Micromorphology, Palynology, and Plastome Analysis
title_full_unstemmed	A Comprehensive Study of the Genus <i>Sanguisorba</i> (Rosaceae) Based on the Floral Micromorphology, Palynology, and Plastome Analysis
title_sort	comprehensive study of the genus <i>sanguisorba</i> (rosaceae) based on the floral micromorphology, palynology, and plastome analysis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bb97ff580108465780adc763de58de3f
work_keys_str_mv	AT inkyupark acomprehensivestudyofthegenusisanguisorbairosaceaebasedonthefloralmicromorphologypalynologyandplastomeanalysis AT junhosong acomprehensivestudyofthegenusisanguisorbairosaceaebasedonthefloralmicromorphologypalynologyandplastomeanalysis AT sungyuyang acomprehensivestudyofthegenusisanguisorbairosaceaebasedonthefloralmicromorphologypalynologyandplastomeanalysis AT goyachoi acomprehensivestudyofthegenusisanguisorbairosaceaebasedonthefloralmicromorphologypalynologyandplastomeanalysis AT byeongcheolmoon acomprehensivestudyofthegenusisanguisorbairosaceaebasedonthefloralmicromorphologypalynologyandplastomeanalysis AT inkyupark comprehensivestudyofthegenusisanguisorbairosaceaebasedonthefloralmicromorphologypalynologyandplastomeanalysis AT junhosong comprehensivestudyofthegenusisanguisorbairosaceaebasedonthefloralmicromorphologypalynologyandplastomeanalysis AT sungyuyang comprehensivestudyofthegenusisanguisorbairosaceaebasedonthefloralmicromorphologypalynologyandplastomeanalysis AT goyachoi comprehensivestudyofthegenusisanguisorbairosaceaebasedonthefloralmicromorphologypalynologyandplastomeanalysis AT byeongcheolmoon comprehensivestudyofthegenusisanguisorbairosaceaebasedonthefloralmicromorphologypalynologyandplastomeanalysis
_version_	1718412128777404416

A Comprehensive Study of the Genus <i>Sanguisorba</i> (Rosaceae) Based on the Floral Micromorphology, Palynology, and Plastome Analysis

Ejemplares similares