Ca extrusion by NCX is compromised in olfactory sensory neurons of OMP mice.

<h4>Background</h4>The role of olfactory marker protein (OMP), a hallmark of mature olfactory sensory neurons (OSNs), has been poorly understood since its discovery. The electrophysiological and behavioral phenotypes of OMP knockout mice indicated that OMP influences olfactory signal tra...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hyun J Kwon, Jae Hyung Koo, Frank Zufall, Trese Leinders-Zufall, Frank L Margolis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bb986dbefd494058bc15bdb250d62e35
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bb986dbefd494058bc15bdb250d62e35
record_format	dspace
spelling	oai:doaj.org-article:bb986dbefd494058bc15bdb250d62e352021-11-25T06:17:40ZCa extrusion by NCX is compromised in olfactory sensory neurons of OMP mice.1932-620310.1371/journal.pone.0004260https://doaj.org/article/bb986dbefd494058bc15bdb250d62e352009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19165324/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The role of olfactory marker protein (OMP), a hallmark of mature olfactory sensory neurons (OSNs), has been poorly understood since its discovery. The electrophysiological and behavioral phenotypes of OMP knockout mice indicated that OMP influences olfactory signal transduction. However, the mechanism by which this occurs remained unknown.<h4>Principal findings</h4>We used intact olfactory epithelium obtained from WT and OMP(-/-) mice to monitor the Ca(2+) dynamics induced by the activation of cyclic nucleotide-gated channels, voltage-operated Ca(2+) channels, or Ca(2+) stores in single dendritic knobs of OSNs. Our data suggested that OMP could act to modulate the Ca(2+)-homeostasis in these neurons by influencing the activity of the plasma membrane Na(+)/Ca(2+)-exchanger (NCX). Immunohistochemistry verifies colocalization of NCX1 and OMP in the cilia and knobs of OSNs. To test the role of NCX activity, we compared the kinetics of Ca(2+) elevation by stimulating the reverse mode of NCX in both WT and OMP(-/-) mice. The resulting Ca(2+) responses indicate that OMP facilitates NCX activity and allows rapid Ca(2+) extrusion from OSN knobs. To address the mechanism by which OMP influences NCX activity in OSNs we studied protein-peptide interactions in real-time using surface plasmon resonance technology. We demonstrate the direct interaction of the XIP regulatory-peptide of NCX with calmodulin (CaM).<h4>Conclusions</h4>Since CaM also binds to the Bex protein, an interacting protein partner of OMP, these observations strongly suggest that OMP can influence CaM efficacy and thus alters NCX activity by a series of protein-protein interactions.Hyun J KwonJae Hyung KooFrank ZufallTrese Leinders-ZufallFrank L MargolisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 1, p e4260 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hyun J Kwon Jae Hyung Koo Frank Zufall Trese Leinders-Zufall Frank L Margolis Ca extrusion by NCX is compromised in olfactory sensory neurons of OMP mice.
description	<h4>Background</h4>The role of olfactory marker protein (OMP), a hallmark of mature olfactory sensory neurons (OSNs), has been poorly understood since its discovery. The electrophysiological and behavioral phenotypes of OMP knockout mice indicated that OMP influences olfactory signal transduction. However, the mechanism by which this occurs remained unknown.<h4>Principal findings</h4>We used intact olfactory epithelium obtained from WT and OMP(-/-) mice to monitor the Ca(2+) dynamics induced by the activation of cyclic nucleotide-gated channels, voltage-operated Ca(2+) channels, or Ca(2+) stores in single dendritic knobs of OSNs. Our data suggested that OMP could act to modulate the Ca(2+)-homeostasis in these neurons by influencing the activity of the plasma membrane Na(+)/Ca(2+)-exchanger (NCX). Immunohistochemistry verifies colocalization of NCX1 and OMP in the cilia and knobs of OSNs. To test the role of NCX activity, we compared the kinetics of Ca(2+) elevation by stimulating the reverse mode of NCX in both WT and OMP(-/-) mice. The resulting Ca(2+) responses indicate that OMP facilitates NCX activity and allows rapid Ca(2+) extrusion from OSN knobs. To address the mechanism by which OMP influences NCX activity in OSNs we studied protein-peptide interactions in real-time using surface plasmon resonance technology. We demonstrate the direct interaction of the XIP regulatory-peptide of NCX with calmodulin (CaM).<h4>Conclusions</h4>Since CaM also binds to the Bex protein, an interacting protein partner of OMP, these observations strongly suggest that OMP can influence CaM efficacy and thus alters NCX activity by a series of protein-protein interactions.
format	article
author	Hyun J Kwon Jae Hyung Koo Frank Zufall Trese Leinders-Zufall Frank L Margolis
author_facet	Hyun J Kwon Jae Hyung Koo Frank Zufall Trese Leinders-Zufall Frank L Margolis
author_sort	Hyun J Kwon
title	Ca extrusion by NCX is compromised in olfactory sensory neurons of OMP mice.
title_short	Ca extrusion by NCX is compromised in olfactory sensory neurons of OMP mice.
title_full	Ca extrusion by NCX is compromised in olfactory sensory neurons of OMP mice.
title_fullStr	Ca extrusion by NCX is compromised in olfactory sensory neurons of OMP mice.
title_full_unstemmed	Ca extrusion by NCX is compromised in olfactory sensory neurons of OMP mice.
title_sort	ca extrusion by ncx is compromised in olfactory sensory neurons of omp mice.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/bb986dbefd494058bc15bdb250d62e35
work_keys_str_mv	AT hyunjkwon caextrusionbyncxiscompromisedinolfactorysensoryneuronsofompmice AT jaehyungkoo caextrusionbyncxiscompromisedinolfactorysensoryneuronsofompmice AT frankzufall caextrusionbyncxiscompromisedinolfactorysensoryneuronsofompmice AT treseleinderszufall caextrusionbyncxiscompromisedinolfactorysensoryneuronsofompmice AT franklmargolis caextrusionbyncxiscompromisedinolfactorysensoryneuronsofompmice
_version_	1718413948232925184

Ca extrusion by NCX is compromised in olfactory sensory neurons of OMP mice.

Ejemplares similares