Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties

Abstract Herein, a novel nanobiocomposite scaffold based on modifying synthesized cross-linked terephthaloyl thiourea-chitosan hydrogel (CTT-CS hydrogel) substrate using the extracted silk fibroin (SF) biopolymer and prepared Mg(OH)2 nanoparticles was designed and synthesized. The biological capacit...

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Autores principales:	Reza Eivazzadeh-Keihan, Fateme Radinekiyan, Hooman Aghamirza Moghim Aliabadi, Sima Sukhtezari, Behnam Tahmasebi, Ali Maleki, Hamid Madanchi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bb9cddef8d69460cb3c81951ec7e0eaa
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spelling	oai:doaj.org-article:bb9cddef8d69460cb3c81951ec7e0eaa2021-12-02T14:01:38ZChitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties10.1038/s41598-020-80133-32045-2322https://doaj.org/article/bb9cddef8d69460cb3c81951ec7e0eaa2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80133-3https://doaj.org/toc/2045-2322Abstract Herein, a novel nanobiocomposite scaffold based on modifying synthesized cross-linked terephthaloyl thiourea-chitosan hydrogel (CTT-CS hydrogel) substrate using the extracted silk fibroin (SF) biopolymer and prepared Mg(OH)2 nanoparticles was designed and synthesized. The biological capacity of this nanobiocomposite scaffold was evaluated by cell viability method, red blood cells hemolytic and anti-biofilm assays. According to the obtained results from 3 and 7 days, the cell viability of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold was accompanied by a considerable increment from 62.5 to 89.6% respectively. Furthermore, its low hemolytic effect (4.5%), and as well, the high anti-biofilm activity and prevention of the P. aeruginosa biofilm formation confirmed its promising hemocompatibility and antibacterial activity. Apart from the cell viability, blood biocompatibility, and antibacterial activity of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold, its structural features were characterized using spectral and analytical techniques (FT-IR, EDX, FE-SEM and TG). As well as, given the mechanical tests, it was indicated that the addition of SF and Mg(OH)2 nanoparticles to the CTT-CS hydrogel could improve its compressive strength from 65.42 to 649.56 kPa.Reza Eivazzadeh-KeihanFateme RadinekiyanHooman Aghamirza Moghim AliabadiSima SukhtezariBehnam TahmasebiAli MalekiHamid MadanchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Reza Eivazzadeh-Keihan Fateme Radinekiyan Hooman Aghamirza Moghim Aliabadi Sima Sukhtezari Behnam Tahmasebi Ali Maleki Hamid Madanchi Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties
description	Abstract Herein, a novel nanobiocomposite scaffold based on modifying synthesized cross-linked terephthaloyl thiourea-chitosan hydrogel (CTT-CS hydrogel) substrate using the extracted silk fibroin (SF) biopolymer and prepared Mg(OH)2 nanoparticles was designed and synthesized. The biological capacity of this nanobiocomposite scaffold was evaluated by cell viability method, red blood cells hemolytic and anti-biofilm assays. According to the obtained results from 3 and 7 days, the cell viability of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold was accompanied by a considerable increment from 62.5 to 89.6% respectively. Furthermore, its low hemolytic effect (4.5%), and as well, the high anti-biofilm activity and prevention of the P. aeruginosa biofilm formation confirmed its promising hemocompatibility and antibacterial activity. Apart from the cell viability, blood biocompatibility, and antibacterial activity of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold, its structural features were characterized using spectral and analytical techniques (FT-IR, EDX, FE-SEM and TG). As well as, given the mechanical tests, it was indicated that the addition of SF and Mg(OH)2 nanoparticles to the CTT-CS hydrogel could improve its compressive strength from 65.42 to 649.56 kPa.
format	article
author	Reza Eivazzadeh-Keihan Fateme Radinekiyan Hooman Aghamirza Moghim Aliabadi Sima Sukhtezari Behnam Tahmasebi Ali Maleki Hamid Madanchi
author_facet	Reza Eivazzadeh-Keihan Fateme Radinekiyan Hooman Aghamirza Moghim Aliabadi Sima Sukhtezari Behnam Tahmasebi Ali Maleki Hamid Madanchi
author_sort	Reza Eivazzadeh-Keihan
title	Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties
title_short	Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties
title_full	Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties
title_fullStr	Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties
title_full_unstemmed	Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties
title_sort	chitosan hydrogel/silk fibroin/mg(oh)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bb9cddef8d69460cb3c81951ec7e0eaa
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Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties

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