Subtype distribution of Blastocystis isolates in Sebha, Libya.

<h4>Background</h4>Blastocystis is a genetically diverse and a common intestinal parasite of humans with a controversial pathogenic potential. This study was carried out to identify the Blastocystis subtypes and their association with demographic and socioeconomic factors among outpatien...

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Autores principales:	Awatif M Abdulsalam, Init Ithoi, Hesham M Al-Mekhlafi, Abdulsalam M Al-Mekhlafi, Abdulhamid Ahmed, Johari Surin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bba35548909f49b485ad7fc500b0e269
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spelling	oai:doaj.org-article:bba35548909f49b485ad7fc500b0e2692021-11-18T08:40:49ZSubtype distribution of Blastocystis isolates in Sebha, Libya.1932-620310.1371/journal.pone.0084372https://doaj.org/article/bba35548909f49b485ad7fc500b0e2692013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24376805/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Blastocystis is a genetically diverse and a common intestinal parasite of humans with a controversial pathogenic potential. This study was carried out to identify the Blastocystis subtypes and their association with demographic and socioeconomic factors among outpatients living in Sebha city, Libya.<h4>Methods/findings</h4>Blastocystis in stool samples were cultured followed by isolation, PCR amplification of a partial SSU rDNA gene, cloning, and sequencing. The DNA sequences of isolated clones showed 98.3% to 100% identity with the reference Blastocystis isolates from the Genbank. Multiple sequence alignment showed polymorphism from one to seven base substitution and/or insertion/deletion in several groups of non-identical nucleotides clones. Phylogenetic analysis revealed three assemblage subtypes (ST) with ST1 as the most prevalent (51.1%) followed by ST2 (24.4%), ST3 (17.8%) and mixed infections of two concurrent subtypes (6.7%).<h4>Blastocystis</h4>ST1 infection was significantly associated with female (P = 0.009) and low educational level (P = 0.034). ST2 was also significantly associated with low educational level (P= 0.008) and ST3 with diarrhoea (P = 0.008).<h4>Conclusion</h4>Phylogenetic analysis of Libyan Blastocystis isolates identified three different subtypes; with ST1 being the predominant subtype and its infection was significantly associated with female gender and low educational level. More extensive studies are needed in order to relate each Blastocystis subtype with clinical symptoms and potential transmission sources in this community.Awatif M AbdulsalamInit IthoiHesham M Al-MekhlafiAbdulsalam M Al-MekhlafiAbdulhamid AhmedJohari SurinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e84372 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Awatif M Abdulsalam Init Ithoi Hesham M Al-Mekhlafi Abdulsalam M Al-Mekhlafi Abdulhamid Ahmed Johari Surin Subtype distribution of Blastocystis isolates in Sebha, Libya.
description	<h4>Background</h4>Blastocystis is a genetically diverse and a common intestinal parasite of humans with a controversial pathogenic potential. This study was carried out to identify the Blastocystis subtypes and their association with demographic and socioeconomic factors among outpatients living in Sebha city, Libya.<h4>Methods/findings</h4>Blastocystis in stool samples were cultured followed by isolation, PCR amplification of a partial SSU rDNA gene, cloning, and sequencing. The DNA sequences of isolated clones showed 98.3% to 100% identity with the reference Blastocystis isolates from the Genbank. Multiple sequence alignment showed polymorphism from one to seven base substitution and/or insertion/deletion in several groups of non-identical nucleotides clones. Phylogenetic analysis revealed three assemblage subtypes (ST) with ST1 as the most prevalent (51.1%) followed by ST2 (24.4%), ST3 (17.8%) and mixed infections of two concurrent subtypes (6.7%).<h4>Blastocystis</h4>ST1 infection was significantly associated with female (P = 0.009) and low educational level (P = 0.034). ST2 was also significantly associated with low educational level (P= 0.008) and ST3 with diarrhoea (P = 0.008).<h4>Conclusion</h4>Phylogenetic analysis of Libyan Blastocystis isolates identified three different subtypes; with ST1 being the predominant subtype and its infection was significantly associated with female gender and low educational level. More extensive studies are needed in order to relate each Blastocystis subtype with clinical symptoms and potential transmission sources in this community.
format	article
author	Awatif M Abdulsalam Init Ithoi Hesham M Al-Mekhlafi Abdulsalam M Al-Mekhlafi Abdulhamid Ahmed Johari Surin
author_facet	Awatif M Abdulsalam Init Ithoi Hesham M Al-Mekhlafi Abdulsalam M Al-Mekhlafi Abdulhamid Ahmed Johari Surin
author_sort	Awatif M Abdulsalam
title	Subtype distribution of Blastocystis isolates in Sebha, Libya.
title_short	Subtype distribution of Blastocystis isolates in Sebha, Libya.
title_full	Subtype distribution of Blastocystis isolates in Sebha, Libya.
title_fullStr	Subtype distribution of Blastocystis isolates in Sebha, Libya.
title_full_unstemmed	Subtype distribution of Blastocystis isolates in Sebha, Libya.
title_sort	subtype distribution of blastocystis isolates in sebha, libya.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/bba35548909f49b485ad7fc500b0e269
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Subtype distribution of Blastocystis isolates in Sebha, Libya.

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