The rumen microbiome inhibits methane formation through dietary choline supplementation
Abstract Enteric fermentation from ruminants is a primary source of anthropogenic methane emission. This study aims to add another approach for methane mitigation by manipulation of the rumen microbiome. Effects of choline supplementation on methane formation were quantified in vitro using the Rumen...
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Nature Portfolio
2021
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oai:doaj.org-article:bba8be0ddc2b4c9384a6b9b9f26ce05d2021-11-08T10:54:38ZThe rumen microbiome inhibits methane formation through dietary choline supplementation10.1038/s41598-021-01031-w2045-2322https://doaj.org/article/bba8be0ddc2b4c9384a6b9b9f26ce05d2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01031-whttps://doaj.org/toc/2045-2322Abstract Enteric fermentation from ruminants is a primary source of anthropogenic methane emission. This study aims to add another approach for methane mitigation by manipulation of the rumen microbiome. Effects of choline supplementation on methane formation were quantified in vitro using the Rumen Simulation Technique. Supplementing 200 mM of choline chloride or choline bicarbonate reduced methane emissions by 97–100% after 15 days. Associated with the reduction of methane formation, metabolomics analysis revealed high post-treatment concentrations of ethanol, which likely served as a major hydrogen sink. Metagenome sequencing showed that the methanogen community was almost entirely lost, and choline-utilizing bacteria that can produce either lactate, ethanol or formate as hydrogen sinks were enriched. The taxa most strongly associated with methane mitigation were Megasphaera elsdenii and Denitrobacterium detoxificans, both capable of consuming lactate, which is an intermediate product and hydrogen sink. Accordingly, choline metabolism promoted the capability of bacteria to utilize alternative hydrogen sinks leading to a decline of hydrogen as a substrate for methane formation. However, fermentation of fibre and total organic matter could not be fully maintained with choline supplementation, while amino acid deamination and ethanolamine catabolism produced excessive ammonia, which would reduce feed efficiency and adversely affect live animal performance.Yang LiMichael KreuzerQuentin ClayssenMarc-Olivier EbertHans-Joachim RuscheweyhShinichi SunagawaCarmen KunzGraeme AttwoodSergej AmelchankaMelissa TerranovaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) |
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Medicine R Science Q Yang Li Michael Kreuzer Quentin Clayssen Marc-Olivier Ebert Hans-Joachim Ruscheweyh Shinichi Sunagawa Carmen Kunz Graeme Attwood Sergej Amelchanka Melissa Terranova The rumen microbiome inhibits methane formation through dietary choline supplementation |
| description |
Abstract Enteric fermentation from ruminants is a primary source of anthropogenic methane emission. This study aims to add another approach for methane mitigation by manipulation of the rumen microbiome. Effects of choline supplementation on methane formation were quantified in vitro using the Rumen Simulation Technique. Supplementing 200 mM of choline chloride or choline bicarbonate reduced methane emissions by 97–100% after 15 days. Associated with the reduction of methane formation, metabolomics analysis revealed high post-treatment concentrations of ethanol, which likely served as a major hydrogen sink. Metagenome sequencing showed that the methanogen community was almost entirely lost, and choline-utilizing bacteria that can produce either lactate, ethanol or formate as hydrogen sinks were enriched. The taxa most strongly associated with methane mitigation were Megasphaera elsdenii and Denitrobacterium detoxificans, both capable of consuming lactate, which is an intermediate product and hydrogen sink. Accordingly, choline metabolism promoted the capability of bacteria to utilize alternative hydrogen sinks leading to a decline of hydrogen as a substrate for methane formation. However, fermentation of fibre and total organic matter could not be fully maintained with choline supplementation, while amino acid deamination and ethanolamine catabolism produced excessive ammonia, which would reduce feed efficiency and adversely affect live animal performance. |
| format |
article |
| author |
Yang Li Michael Kreuzer Quentin Clayssen Marc-Olivier Ebert Hans-Joachim Ruscheweyh Shinichi Sunagawa Carmen Kunz Graeme Attwood Sergej Amelchanka Melissa Terranova |
| author_facet |
Yang Li Michael Kreuzer Quentin Clayssen Marc-Olivier Ebert Hans-Joachim Ruscheweyh Shinichi Sunagawa Carmen Kunz Graeme Attwood Sergej Amelchanka Melissa Terranova |
| author_sort |
Yang Li |
| title |
The rumen microbiome inhibits methane formation through dietary choline supplementation |
| title_short |
The rumen microbiome inhibits methane formation through dietary choline supplementation |
| title_full |
The rumen microbiome inhibits methane formation through dietary choline supplementation |
| title_fullStr |
The rumen microbiome inhibits methane formation through dietary choline supplementation |
| title_full_unstemmed |
The rumen microbiome inhibits methane formation through dietary choline supplementation |
| title_sort |
rumen microbiome inhibits methane formation through dietary choline supplementation |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/bba8be0ddc2b4c9384a6b9b9f26ce05d |
| work_keys_str_mv |
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