Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration

Deliang Zeng,1,2 Xingdi Zhang,3 Xiao Wang,1,2 Lingyan Cao,1 Ao Zheng,1,2 Jiahui Du,1,2 Yongsheng Li,3 Qingfeng Huang,1 Xinquan Jiang1,2 1Department of Prosthodontics, School of Medicine, Ninth People’s Hospital affiliated to Shanghai Jiao Tong University, Shanghai, People’s Repu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zeng D, Zhang X, Wang X, Cao L, Zheng A, Du J, Li Y, Huang Q, Jiang X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	mesoporous Ca-Si-based materials dental pulp cells rat calvarial defect Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bbb88735c8d04ee89dc0ea9044449633
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bbb88735c8d04ee89dc0ea9044449633
record_format	dspace
spelling	oai:doaj.org-article:bbb88735c8d04ee89dc0ea90444496332021-12-02T07:22:52ZFabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration1178-2013https://doaj.org/article/bbb88735c8d04ee89dc0ea90444496332017-11-01T00:00:00Zhttps://www.dovepress.com/fabrication-of-large-pore-mesoporous-ca-si-based-bioceramics-for-bone--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Deliang Zeng,1,2 Xingdi Zhang,3 Xiao Wang,1,2 Lingyan Cao,1 Ao Zheng,1,2 Jiahui Du,1,2 Yongsheng Li,3 Qingfeng Huang,1 Xinquan Jiang1,2 1Department of Prosthodontics, School of Medicine, Ninth People’s Hospital affiliated to Shanghai Jiao Tong University, Shanghai, People’s Republic of China; 2Oral Bioengineering Laboratory, Shanghai Research Institute of Stomatology, School of Medicine, Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, People’s Republic of China; 3Laboratory of Low-Dimensional Materials Chemistry, Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, People’s Republic of China Abstract: Our previous study revealed that mesoporous Ca-Si-based materials exhibited excellent osteoconduction because dissolved ions could form a layer of hydroxycarbonate apatite on the surface of the materials. However, the biological mechanisms underlying bone regeneration were largely unknown. The main aim of this study was to evaluate the osteogenic ability of large-pore mesoporous Ca-Si-based bioceramics (LPMSCs) by alkaline phosphatase assay, real-time PCR analysis, von Kossa, and alizarin red assay. Compared with large-pore mesoporous silica (LPMS), LPMSCs had a better effect on the osteogenic differentiation of dental pulp cells. LPMSC-2 and LPMSC-3 with higher calcium possessed better osteogenic abilities than LPMSC-1, which may be related to the calcium-sensing receptor pathway. Furthermore, the loading capacity for recombinant human platelet-derived growth factor-BB was satisfactory in LPMSCs. In vivo, the areas of new bone formation in the calvarial defect repair were increased in the LPMSC-2 and LPMSC-3 groups compared with the LPMSC-1 and LPMS groups. We concluded that LPMSC-2 and LPMSC-3 possessed both excellent osteogenic abilities and satisfactory loading capacities, which may be attributed to their moderate Ca/Si molar ratio. Therefore, LPMSCs with moderate Ca/Si molar ratio might be potential alterative grafts for craniomaxillofacial bone regeneration. Keywords: mesoporous Ca-Si-based materials, dental pulp cells, rat calvarial defectZeng DZhang XWang XCao LZheng ADu JLi YHuang QJiang XDove Medical Pressarticlemesoporous Ca-Si-based materialsdental pulp cellsrat calvarial defectMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 8277-8287 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	mesoporous Ca-Si-based materials dental pulp cells rat calvarial defect Medicine (General) R5-920
spellingShingle	mesoporous Ca-Si-based materials dental pulp cells rat calvarial defect Medicine (General) R5-920 Zeng D Zhang X Wang X Cao L Zheng A Du J Li Y Huang Q Jiang X Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration
description	Deliang Zeng,1,2 Xingdi Zhang,3 Xiao Wang,1,2 Lingyan Cao,1 Ao Zheng,1,2 Jiahui Du,1,2 Yongsheng Li,3 Qingfeng Huang,1 Xinquan Jiang1,2 1Department of Prosthodontics, School of Medicine, Ninth People’s Hospital affiliated to Shanghai Jiao Tong University, Shanghai, People’s Republic of China; 2Oral Bioengineering Laboratory, Shanghai Research Institute of Stomatology, School of Medicine, Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, People’s Republic of China; 3Laboratory of Low-Dimensional Materials Chemistry, Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, People’s Republic of China Abstract: Our previous study revealed that mesoporous Ca-Si-based materials exhibited excellent osteoconduction because dissolved ions could form a layer of hydroxycarbonate apatite on the surface of the materials. However, the biological mechanisms underlying bone regeneration were largely unknown. The main aim of this study was to evaluate the osteogenic ability of large-pore mesoporous Ca-Si-based bioceramics (LPMSCs) by alkaline phosphatase assay, real-time PCR analysis, von Kossa, and alizarin red assay. Compared with large-pore mesoporous silica (LPMS), LPMSCs had a better effect on the osteogenic differentiation of dental pulp cells. LPMSC-2 and LPMSC-3 with higher calcium possessed better osteogenic abilities than LPMSC-1, which may be related to the calcium-sensing receptor pathway. Furthermore, the loading capacity for recombinant human platelet-derived growth factor-BB was satisfactory in LPMSCs. In vivo, the areas of new bone formation in the calvarial defect repair were increased in the LPMSC-2 and LPMSC-3 groups compared with the LPMSC-1 and LPMS groups. We concluded that LPMSC-2 and LPMSC-3 possessed both excellent osteogenic abilities and satisfactory loading capacities, which may be attributed to their moderate Ca/Si molar ratio. Therefore, LPMSCs with moderate Ca/Si molar ratio might be potential alterative grafts for craniomaxillofacial bone regeneration. Keywords: mesoporous Ca-Si-based materials, dental pulp cells, rat calvarial defect
format	article
author	Zeng D Zhang X Wang X Cao L Zheng A Du J Li Y Huang Q Jiang X
author_facet	Zeng D Zhang X Wang X Cao L Zheng A Du J Li Y Huang Q Jiang X
author_sort	Zeng D
title	Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration
title_short	Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration
title_full	Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration
title_fullStr	Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration
title_full_unstemmed	Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration
title_sort	fabrication of large-pore mesoporous ca-si-based bioceramics for bone regeneration
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/bbb88735c8d04ee89dc0ea9044449633
work_keys_str_mv	AT zengd fabricationoflargeporemesoporouscasibasedbioceramicsforboneregeneration AT zhangx fabricationoflargeporemesoporouscasibasedbioceramicsforboneregeneration AT wangx fabricationoflargeporemesoporouscasibasedbioceramicsforboneregeneration AT caol fabricationoflargeporemesoporouscasibasedbioceramicsforboneregeneration AT zhenga fabricationoflargeporemesoporouscasibasedbioceramicsforboneregeneration AT duj fabricationoflargeporemesoporouscasibasedbioceramicsforboneregeneration AT liy fabricationoflargeporemesoporouscasibasedbioceramicsforboneregeneration AT huangq fabricationoflargeporemesoporouscasibasedbioceramicsforboneregeneration AT jiangx fabricationoflargeporemesoporouscasibasedbioceramicsforboneregeneration
_version_	1718399475879247872

Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration

Ejemplares similares