Osr1 Is Required for Mesenchymal Derivatives That Produce Collagen in the Bladder

The extracellular matrix of the bladder consists mostly of type I and III collagen, which are required during loading. During bladder injury, there is an accumulation of collagen that impairs bladder function. Little is known about the genes that regulate production of collagens in the bladder. We d...

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Autores principales:	Vasikar Murugapoopathy, Philippe G. Cammisotto, Abubakr H. Mossa, Lysanne Campeau, Indra R. Gupta
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Odd1 extracellular matrix bladder disease bladder development Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/bbb9b23df9b442c2b28932d4f800e668
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spelling	oai:doaj.org-article:bbb9b23df9b442c2b28932d4f800e6682021-11-25T17:56:09ZOsr1 Is Required for Mesenchymal Derivatives That Produce Collagen in the Bladder10.3390/ijms2222123871422-00671661-6596https://doaj.org/article/bbb9b23df9b442c2b28932d4f800e6682021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12387https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The extracellular matrix of the bladder consists mostly of type I and III collagen, which are required during loading. During bladder injury, there is an accumulation of collagen that impairs bladder function. Little is known about the genes that regulate production of collagens in the bladder. We demonstrate that the transcription factor Odd-skipped related 1 (Osr1) is expressed in the bladder mesenchyme and epithelium at the onset of development. As development proceeds, Osr1 is mainly expressed in mesenchymal progenitors and their derivatives. We hypothesized that Osr1 regulates mesenchymal cell differentiation and production of collagens in the bladder. To test this hypothesis, we examined newborn and adult mice heterozygous for <i>Osr1</i>, <i>Osr1<sup>+/−</sup></i>. The bladders of newborn <i>Osr1<sup>+/−</sup></i> mice had a decrease in collagen I by western blot analysis and a global decrease in collagens using Sirius red staining. There was also a decrease in the cellularity of the lamina propria, where most collagen is synthesized. This was not due to decreased proliferation or increased apoptosis in this cell population. Surprisingly, the bladders of adult <i>Osr1<sup>+/−</sup></i> mice had an increase in collagen that was associated with abnormal bladder function; they also had a decrease in bladder capacity and voided more frequently. The results suggest that Osr1 is important for the differentiation of mesenchymal cells that give rise to collagen-producing cells.Vasikar MurugapoopathyPhilippe G. CammisottoAbubakr H. MossaLysanne CampeauIndra R. GuptaMDPI AGarticleOdd1extracellular matrixbladder diseasebladder developmentBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12387, p 12387 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Odd1 extracellular matrix bladder disease bladder development Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	Odd1 extracellular matrix bladder disease bladder development Biology (General) QH301-705.5 Chemistry QD1-999 Vasikar Murugapoopathy Philippe G. Cammisotto Abubakr H. Mossa Lysanne Campeau Indra R. Gupta Osr1 Is Required for Mesenchymal Derivatives That Produce Collagen in the Bladder
description	The extracellular matrix of the bladder consists mostly of type I and III collagen, which are required during loading. During bladder injury, there is an accumulation of collagen that impairs bladder function. Little is known about the genes that regulate production of collagens in the bladder. We demonstrate that the transcription factor Odd-skipped related 1 (Osr1) is expressed in the bladder mesenchyme and epithelium at the onset of development. As development proceeds, Osr1 is mainly expressed in mesenchymal progenitors and their derivatives. We hypothesized that Osr1 regulates mesenchymal cell differentiation and production of collagens in the bladder. To test this hypothesis, we examined newborn and adult mice heterozygous for <i>Osr1</i>, <i>Osr1<sup>+/−</sup></i>. The bladders of newborn <i>Osr1<sup>+/−</sup></i> mice had a decrease in collagen I by western blot analysis and a global decrease in collagens using Sirius red staining. There was also a decrease in the cellularity of the lamina propria, where most collagen is synthesized. This was not due to decreased proliferation or increased apoptosis in this cell population. Surprisingly, the bladders of adult <i>Osr1<sup>+/−</sup></i> mice had an increase in collagen that was associated with abnormal bladder function; they also had a decrease in bladder capacity and voided more frequently. The results suggest that Osr1 is important for the differentiation of mesenchymal cells that give rise to collagen-producing cells.
format	article
author	Vasikar Murugapoopathy Philippe G. Cammisotto Abubakr H. Mossa Lysanne Campeau Indra R. Gupta
author_facet	Vasikar Murugapoopathy Philippe G. Cammisotto Abubakr H. Mossa Lysanne Campeau Indra R. Gupta
author_sort	Vasikar Murugapoopathy
title	Osr1 Is Required for Mesenchymal Derivatives That Produce Collagen in the Bladder
title_short	Osr1 Is Required for Mesenchymal Derivatives That Produce Collagen in the Bladder
title_full	Osr1 Is Required for Mesenchymal Derivatives That Produce Collagen in the Bladder
title_fullStr	Osr1 Is Required for Mesenchymal Derivatives That Produce Collagen in the Bladder
title_full_unstemmed	Osr1 Is Required for Mesenchymal Derivatives That Produce Collagen in the Bladder
title_sort	osr1 is required for mesenchymal derivatives that produce collagen in the bladder
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bbb9b23df9b442c2b28932d4f800e668
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Osr1 Is Required for Mesenchymal Derivatives That Produce Collagen in the Bladder

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