Phloroglucinol-Gold and -Zinc Oxide Nanoparticles: Antibiofilm and Antivirulence Activities towards <i>Pseudomonas</i> <i>aeruginosa</i> PAO1

With the advancement of nanotechnology, several nanoparticles have been synthesized as antimicrobial agents by utilizing biologically derived materials. In most cases, the materials used for the synthesis of nanoparticles from natural sources are extracts. Natural extracts contain a wide range of bi...

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Autores principales:	Fazlurrahman Khan, Min-Gyun Kang, Du-Min Jo, Pathum Chandika, Won-Kyo Jung, Hyun Wook Kang, Young-Mog Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antibiofilm antivirulence phloroglucinol PG-AuNPs PG-ZnONPs nanoparticles Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bbc0cd17b57046d08665e76e4cbb55d7
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spelling	oai:doaj.org-article:bbc0cd17b57046d08665e76e4cbb55d72021-11-25T18:12:44ZPhloroglucinol-Gold and -Zinc Oxide Nanoparticles: Antibiofilm and Antivirulence Activities towards <i>Pseudomonas</i> <i>aeruginosa</i> PAO110.3390/md191106011660-3397https://doaj.org/article/bbc0cd17b57046d08665e76e4cbb55d72021-10-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/601https://doaj.org/toc/1660-3397With the advancement of nanotechnology, several nanoparticles have been synthesized as antimicrobial agents by utilizing biologically derived materials. In most cases, the materials used for the synthesis of nanoparticles from natural sources are extracts. Natural extracts contain a wide range of bioactive components, making it difficult to pinpoint the exact component responsible for nanoparticle synthesis. Furthermore, the bioactive component present in the extract changes according to numerous environmental factors. As a result, the current work intended to synthesize gold (AuNPs) and zinc oxide (ZnONPs) nanoparticles using pure phloroglucinol (PG). The synthesized PG-AuNPs and PG-ZnONPs were characterized using a UV–Vis absorption spectrophotometer, FTIR, DLS, FE-TEM, zeta potential, EDS, and energy-dispersive X-ray diffraction. The characterized PG-AuNPs and PG-ZnONPs have been employed to combat the pathogenesis of <i>Pseudomonas aeruginosa</i>. <i>P</i>. <i>aeruginosa</i> is recognized as one of the most prevalent pathogens responsible for the common cause of nosocomial infection in humans. Antimicrobial resistance in <i>P</i>. <i>aeruginosa</i> has been linked to the development of recalcitrant phenotypic characteristics, such as biofilm, which has been identified as one of the major obstacles to antimicrobial therapy. Furthermore, <i>P</i>. <i>aeruginosa</i> generates various virulence factors that are a major cause of chronic infection. These PG-AuNPs and PG-ZnONPs significantly inhibit early stage biofilm and eradicate mature biofilm. Furthermore, these NPs reduce <i>P</i>. <i>aeruginosa</i> virulence factors such as pyoverdine, pyocyanin, protease, rhamnolipid, and hemolytic capabilities. In addition, these NPs significantly reduce <i>P</i>. <i>aeruginosa</i> swarming, swimming, and twitching motility. PG-AuNPs and PG-ZnONPs can be used as control agents for infections caused by the biofilm-forming human pathogenic bacterium <i>P</i>. <i>aeruginosa</i>.Fazlurrahman KhanMin-Gyun KangDu-Min JoPathum ChandikaWon-Kyo JungHyun Wook KangYoung-Mog KimMDPI AGarticleantibiofilmantivirulencephloroglucinolPG-AuNPsPG-ZnONPsnanoparticlesBiology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 601, p 601 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiofilm antivirulence phloroglucinol PG-AuNPs PG-ZnONPs nanoparticles Biology (General) QH301-705.5
spellingShingle	antibiofilm antivirulence phloroglucinol PG-AuNPs PG-ZnONPs nanoparticles Biology (General) QH301-705.5 Fazlurrahman Khan Min-Gyun Kang Du-Min Jo Pathum Chandika Won-Kyo Jung Hyun Wook Kang Young-Mog Kim Phloroglucinol-Gold and -Zinc Oxide Nanoparticles: Antibiofilm and Antivirulence Activities towards <i>Pseudomonas</i> <i>aeruginosa</i> PAO1
description	With the advancement of nanotechnology, several nanoparticles have been synthesized as antimicrobial agents by utilizing biologically derived materials. In most cases, the materials used for the synthesis of nanoparticles from natural sources are extracts. Natural extracts contain a wide range of bioactive components, making it difficult to pinpoint the exact component responsible for nanoparticle synthesis. Furthermore, the bioactive component present in the extract changes according to numerous environmental factors. As a result, the current work intended to synthesize gold (AuNPs) and zinc oxide (ZnONPs) nanoparticles using pure phloroglucinol (PG). The synthesized PG-AuNPs and PG-ZnONPs were characterized using a UV–Vis absorption spectrophotometer, FTIR, DLS, FE-TEM, zeta potential, EDS, and energy-dispersive X-ray diffraction. The characterized PG-AuNPs and PG-ZnONPs have been employed to combat the pathogenesis of <i>Pseudomonas aeruginosa</i>. <i>P</i>. <i>aeruginosa</i> is recognized as one of the most prevalent pathogens responsible for the common cause of nosocomial infection in humans. Antimicrobial resistance in <i>P</i>. <i>aeruginosa</i> has been linked to the development of recalcitrant phenotypic characteristics, such as biofilm, which has been identified as one of the major obstacles to antimicrobial therapy. Furthermore, <i>P</i>. <i>aeruginosa</i> generates various virulence factors that are a major cause of chronic infection. These PG-AuNPs and PG-ZnONPs significantly inhibit early stage biofilm and eradicate mature biofilm. Furthermore, these NPs reduce <i>P</i>. <i>aeruginosa</i> virulence factors such as pyoverdine, pyocyanin, protease, rhamnolipid, and hemolytic capabilities. In addition, these NPs significantly reduce <i>P</i>. <i>aeruginosa</i> swarming, swimming, and twitching motility. PG-AuNPs and PG-ZnONPs can be used as control agents for infections caused by the biofilm-forming human pathogenic bacterium <i>P</i>. <i>aeruginosa</i>.
format	article
author	Fazlurrahman Khan Min-Gyun Kang Du-Min Jo Pathum Chandika Won-Kyo Jung Hyun Wook Kang Young-Mog Kim
author_facet	Fazlurrahman Khan Min-Gyun Kang Du-Min Jo Pathum Chandika Won-Kyo Jung Hyun Wook Kang Young-Mog Kim
author_sort	Fazlurrahman Khan
title	Phloroglucinol-Gold and -Zinc Oxide Nanoparticles: Antibiofilm and Antivirulence Activities towards <i>Pseudomonas</i> <i>aeruginosa</i> PAO1
title_short	Phloroglucinol-Gold and -Zinc Oxide Nanoparticles: Antibiofilm and Antivirulence Activities towards <i>Pseudomonas</i> <i>aeruginosa</i> PAO1
title_full	Phloroglucinol-Gold and -Zinc Oxide Nanoparticles: Antibiofilm and Antivirulence Activities towards <i>Pseudomonas</i> <i>aeruginosa</i> PAO1
title_fullStr	Phloroglucinol-Gold and -Zinc Oxide Nanoparticles: Antibiofilm and Antivirulence Activities towards <i>Pseudomonas</i> <i>aeruginosa</i> PAO1
title_full_unstemmed	Phloroglucinol-Gold and -Zinc Oxide Nanoparticles: Antibiofilm and Antivirulence Activities towards <i>Pseudomonas</i> <i>aeruginosa</i> PAO1
title_sort	phloroglucinol-gold and -zinc oxide nanoparticles: antibiofilm and antivirulence activities towards <i>pseudomonas</i> <i>aeruginosa</i> pao1
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bbc0cd17b57046d08665e76e4cbb55d7
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Phloroglucinol-Gold and -Zinc Oxide Nanoparticles: Antibiofilm and Antivirulence Activities towards <i>Pseudomonas</i> <i>aeruginosa</i> PAO1

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