Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors

Elucidating how synaptic molecules such as AMPA receptors mediate neuronal communication and tracking their dynamic expression during behavior is crucial to understand cognition and disease, but current technological barriers preclude large-scale exploration of molecular dynamics in vivo. We have de...

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Autores principales:	Austin R Graves, Richard H Roth, Han L Tan, Qianwen Zhu, Alexei M Bygrave, Elena Lopez-Ortega, Ingie Hong, Alina C Spiegel, Richard C Johnson, Joshua T Vogelstein, Daniel J Tward, Michael I Miller, Richard L Huganir
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	synapse plasticity in vivo imaging AMPA receptor behavior Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bbc936d28bba4fee99a9525f2f0b9c39
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spelling	oai:doaj.org-article:bbc936d28bba4fee99a9525f2f0b9c392021-11-25T08:40:43ZVisualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors10.7554/eLife.668092050-084Xe66809https://doaj.org/article/bbc936d28bba4fee99a9525f2f0b9c392021-10-01T00:00:00Zhttps://elifesciences.org/articles/66809https://doaj.org/toc/2050-084XElucidating how synaptic molecules such as AMPA receptors mediate neuronal communication and tracking their dynamic expression during behavior is crucial to understand cognition and disease, but current technological barriers preclude large-scale exploration of molecular dynamics in vivo. We have developed a suite of innovative methodologies that break through these barriers: a new knockin mouse line with fluorescently tagged endogenous AMPA receptors, two-photon imaging of hundreds of thousands of labeled synapses in behaving mice, and computer vision-based automatic synapse detection. Using these tools, we can longitudinally track how the strength of populations of synapses changes during behavior. We used this approach to generate an unprecedentedly detailed spatiotemporal map of synapses undergoing changes in strength following sensory experience. More generally, these tools can be used as an optical probe capable of measuring functional synapse strength across entire brain areas during any behavioral paradigm, describing complex system-wide changes with molecular precision.Austin R GravesRichard H RothHan L TanQianwen ZhuAlexei M BygraveElena Lopez-OrtegaIngie HongAlina C SpiegelRichard C JohnsonJoshua T VogelsteinDaniel J TwardMichael I MillerRichard L HuganireLife Sciences Publications Ltdarticlesynapseplasticityin vivo imagingAMPA receptorbehaviorMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	synapse plasticity in vivo imaging AMPA receptor behavior Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	synapse plasticity in vivo imaging AMPA receptor behavior Medicine R Science Q Biology (General) QH301-705.5 Austin R Graves Richard H Roth Han L Tan Qianwen Zhu Alexei M Bygrave Elena Lopez-Ortega Ingie Hong Alina C Spiegel Richard C Johnson Joshua T Vogelstein Daniel J Tward Michael I Miller Richard L Huganir Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors
description	Elucidating how synaptic molecules such as AMPA receptors mediate neuronal communication and tracking their dynamic expression during behavior is crucial to understand cognition and disease, but current technological barriers preclude large-scale exploration of molecular dynamics in vivo. We have developed a suite of innovative methodologies that break through these barriers: a new knockin mouse line with fluorescently tagged endogenous AMPA receptors, two-photon imaging of hundreds of thousands of labeled synapses in behaving mice, and computer vision-based automatic synapse detection. Using these tools, we can longitudinally track how the strength of populations of synapses changes during behavior. We used this approach to generate an unprecedentedly detailed spatiotemporal map of synapses undergoing changes in strength following sensory experience. More generally, these tools can be used as an optical probe capable of measuring functional synapse strength across entire brain areas during any behavioral paradigm, describing complex system-wide changes with molecular precision.
format	article
author	Austin R Graves Richard H Roth Han L Tan Qianwen Zhu Alexei M Bygrave Elena Lopez-Ortega Ingie Hong Alina C Spiegel Richard C Johnson Joshua T Vogelstein Daniel J Tward Michael I Miller Richard L Huganir
author_facet	Austin R Graves Richard H Roth Han L Tan Qianwen Zhu Alexei M Bygrave Elena Lopez-Ortega Ingie Hong Alina C Spiegel Richard C Johnson Joshua T Vogelstein Daniel J Tward Michael I Miller Richard L Huganir
author_sort	Austin R Graves
title	Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors
title_short	Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors
title_full	Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors
title_fullStr	Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors
title_full_unstemmed	Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors
title_sort	visualizing synaptic plasticity in vivo by large-scale imaging of endogenous ampa receptors
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/bbc936d28bba4fee99a9525f2f0b9c39
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Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors

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