Nanoparticles prepared from the water extract of Gusuibu (Drynaria fortunei J. Sm.) protects osteoblasts against insults and promotes cell maturation

Chung-King Hsu1,2, Mei-Hsiu Liao3, Yu-Tyng Tai4, Shing-Hwa Liu5, Keng-Liang Ou6, Hsu-Wei Fang7, I-Jung Lee8, Ruei-Ming Chen2,31Institute of Materials Science and Engineering, National Taipei University of Technology, 2Cell Physiology and Molecular Image Research Center, Taipei Medical University-Wan...

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Autores principales:	Hsu C-K, Liao M-H, Tai Y-T, Liu S-H, Ou K-L, Fang H-W, Lee I-J, Chen R-M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bbcab52f97f54d2f88d0b44662580eda
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spelling	oai:doaj.org-article:bbcab52f97f54d2f88d0b44662580eda2021-12-02T02:10:26ZNanoparticles prepared from the water extract of Gusuibu (Drynaria fortunei J. Sm.) protects osteoblasts against insults and promotes cell maturation1176-91141178-2013https://doaj.org/article/bbcab52f97f54d2f88d0b44662580eda2011-07-01T00:00:00Zhttp://www.dovepress.com/nanoparticles-prepared-from-the-water-extract-of-gusuibu-drynaria-fort-a7816https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Chung-King Hsu1,2, Mei-Hsiu Liao3, Yu-Tyng Tai4, Shing-Hwa Liu5, Keng-Liang Ou6, Hsu-Wei Fang7, I-Jung Lee8, Ruei-Ming Chen2,31Institute of Materials Science and Engineering, National Taipei University of Technology, 2Cell Physiology and Molecular Image Research Center, Taipei Medical University-Wan Fang Medical Center, 3Graduate Institute of Medical Sciences, Taipei Medical University, 4Department of Anesthesiology, Taipei Medical University-Wan Fang Medical Center, 5Institute of Toxicology, College of Medicine, National Taiwan University, 6Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, 7Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 8Division of Information and Herbarium, National Research Institute of Chinese Medicine, Taipei, TaiwanAbstract: Our previous study showed that Gusuibu (Drynaria fortunei J. Sm.) can stimulate osteoblast maturation. This study was further designed to evaluate the effects of nanoparticles prepared from the water extract of Gusuibu (WEG) on osteoblast survival and maturation. Primary osteoblasts were exposed to 1, 10, 100, and 1000 &micro;g/mL nanoparticles of WEG (nWEG) for 24, 48, and 72 hours did not affect morphologies, viability, or apoptosis of osteoblasts. In comparison, treatment of osteoblasts with 1000 &micro;g/mL WEG for 72 hours decreased cell viability and induced DNA fragmentation and cell apoptosis. nWEG had better antioxidant bioactivity in protecting osteoblasts from oxidative and nitrosative stress-induced apoptosis than WEG. In addition, nWEG stimulated greater osteoblast maturation than did WEG. Therefore, this study shows that WEG nanoparticles are safer to primary osteoblasts than are normal-sized products, and may promote better bone healing by protecting osteoblasts from apoptotic insults, and by promoting osteogenic maturation.Keywords: Gusuibu, nanoparticles, cell protection, osteoblast maturationHsu C-KLiao M-HTai Y-TLiu S-HOu K-LFang H-WLee I-JChen R-MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1405-1413 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Hsu C-K Liao M-H Tai Y-T Liu S-H Ou K-L Fang H-W Lee I-J Chen R-M Nanoparticles prepared from the water extract of Gusuibu (Drynaria fortunei J. Sm.) protects osteoblasts against insults and promotes cell maturation
description	Chung-King Hsu1,2, Mei-Hsiu Liao3, Yu-Tyng Tai4, Shing-Hwa Liu5, Keng-Liang Ou6, Hsu-Wei Fang7, I-Jung Lee8, Ruei-Ming Chen2,31Institute of Materials Science and Engineering, National Taipei University of Technology, 2Cell Physiology and Molecular Image Research Center, Taipei Medical University-Wan Fang Medical Center, 3Graduate Institute of Medical Sciences, Taipei Medical University, 4Department of Anesthesiology, Taipei Medical University-Wan Fang Medical Center, 5Institute of Toxicology, College of Medicine, National Taiwan University, 6Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, 7Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 8Division of Information and Herbarium, National Research Institute of Chinese Medicine, Taipei, TaiwanAbstract: Our previous study showed that Gusuibu (Drynaria fortunei J. Sm.) can stimulate osteoblast maturation. This study was further designed to evaluate the effects of nanoparticles prepared from the water extract of Gusuibu (WEG) on osteoblast survival and maturation. Primary osteoblasts were exposed to 1, 10, 100, and 1000 &micro;g/mL nanoparticles of WEG (nWEG) for 24, 48, and 72 hours did not affect morphologies, viability, or apoptosis of osteoblasts. In comparison, treatment of osteoblasts with 1000 &micro;g/mL WEG for 72 hours decreased cell viability and induced DNA fragmentation and cell apoptosis. nWEG had better antioxidant bioactivity in protecting osteoblasts from oxidative and nitrosative stress-induced apoptosis than WEG. In addition, nWEG stimulated greater osteoblast maturation than did WEG. Therefore, this study shows that WEG nanoparticles are safer to primary osteoblasts than are normal-sized products, and may promote better bone healing by protecting osteoblasts from apoptotic insults, and by promoting osteogenic maturation.Keywords: Gusuibu, nanoparticles, cell protection, osteoblast maturation
format	article
author	Hsu C-K Liao M-H Tai Y-T Liu S-H Ou K-L Fang H-W Lee I-J Chen R-M
author_facet	Hsu C-K Liao M-H Tai Y-T Liu S-H Ou K-L Fang H-W Lee I-J Chen R-M
author_sort	Hsu C-K
title	Nanoparticles prepared from the water extract of Gusuibu (Drynaria fortunei J. Sm.) protects osteoblasts against insults and promotes cell maturation
title_short	Nanoparticles prepared from the water extract of Gusuibu (Drynaria fortunei J. Sm.) protects osteoblasts against insults and promotes cell maturation
title_full	Nanoparticles prepared from the water extract of Gusuibu (Drynaria fortunei J. Sm.) protects osteoblasts against insults and promotes cell maturation
title_fullStr	Nanoparticles prepared from the water extract of Gusuibu (Drynaria fortunei J. Sm.) protects osteoblasts against insults and promotes cell maturation
title_full_unstemmed	Nanoparticles prepared from the water extract of Gusuibu (Drynaria fortunei J. Sm.) protects osteoblasts against insults and promotes cell maturation
title_sort	nanoparticles prepared from the water extract of gusuibu (drynaria fortunei j. sm.) protects osteoblasts against insults and promotes cell maturation
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/bbcab52f97f54d2f88d0b44662580eda
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Nanoparticles prepared from the water extract of Gusuibu (Drynaria fortunei J. Sm.) protects osteoblasts against insults and promotes cell maturation

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