Natural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato

Abstract Abscission is the mechanism by which plants disconnect unfertilized flowers, ripe fruits, senescent or diseased organs from the plant. In tomato, pedicel abscission is an important agronomic factor that controls yield and post-harvest fruit quality. Two non-allelic mutations, jointless (j)...

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Autores principales:	Maria Victoria Gomez Roldan, Claire Périlleux, Halima Morin, Samuel Huerga-Fernandez, David Latrasse, Moussa Benhamed, Abdelhafid Bendahmane
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bbd7ca87efbf4483b91a001e6deb397c
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spelling	oai:doaj.org-article:bbd7ca87efbf4483b91a001e6deb397c2021-12-02T12:32:55ZNatural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato10.1038/s41598-017-04556-12045-2322https://doaj.org/article/bbd7ca87efbf4483b91a001e6deb397c2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04556-1https://doaj.org/toc/2045-2322Abstract Abscission is the mechanism by which plants disconnect unfertilized flowers, ripe fruits, senescent or diseased organs from the plant. In tomato, pedicel abscission is an important agronomic factor that controls yield and post-harvest fruit quality. Two non-allelic mutations, jointless (j) and jointless-2 (j-2), controlling pedicel abscission zone formation have been documented but only j-2 has been extensively used in breeding. J was shown to encode a MADS-box protein. Using a combination of physical mapping and gene expression analysis we identified a positional candidate, Solyc12g038510, associated with j-2 phenotype. Targeted knockout of Solyc12g038510, using CRISPR/Cas9 system, validated our hypothesis. Solyc12g038510 encodes the MADS-box protein SlMBP21. Molecular analysis of j-2 natural variation revealed two independent loss-of-function mutants. The first results of an insertion of a Rider retrotransposable element. The second results of a stop codon mutation that leads to a truncated protein form. To bring new insights into the role of J and J-2 in abscission zone formation, we phenotyped the single and the double mutants and the engineered alleles. We showed that J is epistatic to J-2 and that the branched inflorescences and the leafy sepals observed in accessions harboring j-2 alleles are likely the consequences of linkage drags.Maria Victoria Gomez RoldanClaire PérilleuxHalima MorinSamuel Huerga-FernandezDavid LatrasseMoussa BenhamedAbdelhafid BendahmaneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Victoria Gomez Roldan Claire Périlleux Halima Morin Samuel Huerga-Fernandez David Latrasse Moussa Benhamed Abdelhafid Bendahmane Natural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato
description	Abstract Abscission is the mechanism by which plants disconnect unfertilized flowers, ripe fruits, senescent or diseased organs from the plant. In tomato, pedicel abscission is an important agronomic factor that controls yield and post-harvest fruit quality. Two non-allelic mutations, jointless (j) and jointless-2 (j-2), controlling pedicel abscission zone formation have been documented but only j-2 has been extensively used in breeding. J was shown to encode a MADS-box protein. Using a combination of physical mapping and gene expression analysis we identified a positional candidate, Solyc12g038510, associated with j-2 phenotype. Targeted knockout of Solyc12g038510, using CRISPR/Cas9 system, validated our hypothesis. Solyc12g038510 encodes the MADS-box protein SlMBP21. Molecular analysis of j-2 natural variation revealed two independent loss-of-function mutants. The first results of an insertion of a Rider retrotransposable element. The second results of a stop codon mutation that leads to a truncated protein form. To bring new insights into the role of J and J-2 in abscission zone formation, we phenotyped the single and the double mutants and the engineered alleles. We showed that J is epistatic to J-2 and that the branched inflorescences and the leafy sepals observed in accessions harboring j-2 alleles are likely the consequences of linkage drags.
format	article
author	Maria Victoria Gomez Roldan Claire Périlleux Halima Morin Samuel Huerga-Fernandez David Latrasse Moussa Benhamed Abdelhafid Bendahmane
author_facet	Maria Victoria Gomez Roldan Claire Périlleux Halima Morin Samuel Huerga-Fernandez David Latrasse Moussa Benhamed Abdelhafid Bendahmane
author_sort	Maria Victoria Gomez Roldan
title	Natural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato
title_short	Natural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato
title_full	Natural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato
title_fullStr	Natural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato
title_full_unstemmed	Natural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato
title_sort	natural and induced loss of function mutations in slmbp21 mads-box gene led to jointless-2 phenotype in tomato
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bbd7ca87efbf4483b91a001e6deb397c
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Natural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato

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