Altered <i>O</i>-glycomes of Renal Brush-Border Membrane in Model Rats with Chronic Kidney Diseases

Chronic kidney disease (CKD) is defined as a decrease in renal function or glomerular filtration rate (GFR), and proteinuria is often present. Proteinuria increases with age and can be caused by glomerular and/or proximal tubule (PT) alterations. PT cells have an apical brush border membrane (BBM),...

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Autores principales:	Aiying Yu, Jingfu Zhao, Jieqiang Zhong, Junyao Wang, Shiv Pratap S. Yadav, Bruce A. Molitoris, Mark C. Wagner, Yehia Mechref
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>O</i>-glycan brush-border membrane proteinuria and hypertension obese and diabetic chronic kidney disease differential expression analysis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/bbd8f5c80fb74ce9bf556ae79c00c53e
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spelling	oai:doaj.org-article:bbd8f5c80fb74ce9bf556ae79c00c53e2021-11-25T16:51:52ZAltered <i>O</i>-glycomes of Renal Brush-Border Membrane in Model Rats with Chronic Kidney Diseases10.3390/biom111115602218-273Xhttps://doaj.org/article/bbd8f5c80fb74ce9bf556ae79c00c53e2021-10-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1560https://doaj.org/toc/2218-273XChronic kidney disease (CKD) is defined as a decrease in renal function or glomerular filtration rate (GFR), and proteinuria is often present. Proteinuria increases with age and can be caused by glomerular and/or proximal tubule (PT) alterations. PT cells have an apical brush border membrane (BBM), which is a highly dynamic, organized, and specialized membrane region containing multiple glycoproteins required for its functions including regulating uptake, secretion, and signaling dependent upon the physiologic state. PT disorders contribute to the dysfunction observed in CKD. Many glycoprotein functions have been attributed to their <i>N</i>- and <i>O</i>-glycans, which are highly regulated and complex. In this study, the <i>O</i>-glycans present in rat BBMs from animals with different levels of kidney disease and proteinuria were characterized and analyzed using liquid chromatography tandem mass spectrometry (LC–MS/MS). A principal component analysis (PCA) documented that each group has distinct <i>O</i>-glycan distributions. Higher fucosylation levels were observed in the CKD and diabetic groups, which may contribute to PT dysfunction by altering physiologic glycoprotein interactions. Fucosylated <i>O</i>-glycans such as 1-1-1-0 exhibited higher abundance in the severe proteinuric groups. These glycomic results revealed that differential <i>O</i>-glycan expressions in CKD progressions has the potential to define the mechanism of proteinuria in kidney disease and to identify potential therapeutic interventions.Aiying YuJingfu ZhaoJieqiang ZhongJunyao WangShiv Pratap S. YadavBruce A. MolitorisMark C. WagnerYehia MechrefMDPI AGarticle<i>O</i>-glycanbrush-border membraneproteinuria and hypertensionobese and diabeticchronic kidney diseasedifferential expression analysisMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1560, p 1560 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>O</i>-glycan brush-border membrane proteinuria and hypertension obese and diabetic chronic kidney disease differential expression analysis Microbiology QR1-502
spellingShingle	<i>O</i>-glycan brush-border membrane proteinuria and hypertension obese and diabetic chronic kidney disease differential expression analysis Microbiology QR1-502 Aiying Yu Jingfu Zhao Jieqiang Zhong Junyao Wang Shiv Pratap S. Yadav Bruce A. Molitoris Mark C. Wagner Yehia Mechref Altered <i>O</i>-glycomes of Renal Brush-Border Membrane in Model Rats with Chronic Kidney Diseases
description	Chronic kidney disease (CKD) is defined as a decrease in renal function or glomerular filtration rate (GFR), and proteinuria is often present. Proteinuria increases with age and can be caused by glomerular and/or proximal tubule (PT) alterations. PT cells have an apical brush border membrane (BBM), which is a highly dynamic, organized, and specialized membrane region containing multiple glycoproteins required for its functions including regulating uptake, secretion, and signaling dependent upon the physiologic state. PT disorders contribute to the dysfunction observed in CKD. Many glycoprotein functions have been attributed to their <i>N</i>- and <i>O</i>-glycans, which are highly regulated and complex. In this study, the <i>O</i>-glycans present in rat BBMs from animals with different levels of kidney disease and proteinuria were characterized and analyzed using liquid chromatography tandem mass spectrometry (LC–MS/MS). A principal component analysis (PCA) documented that each group has distinct <i>O</i>-glycan distributions. Higher fucosylation levels were observed in the CKD and diabetic groups, which may contribute to PT dysfunction by altering physiologic glycoprotein interactions. Fucosylated <i>O</i>-glycans such as 1-1-1-0 exhibited higher abundance in the severe proteinuric groups. These glycomic results revealed that differential <i>O</i>-glycan expressions in CKD progressions has the potential to define the mechanism of proteinuria in kidney disease and to identify potential therapeutic interventions.
format	article
author	Aiying Yu Jingfu Zhao Jieqiang Zhong Junyao Wang Shiv Pratap S. Yadav Bruce A. Molitoris Mark C. Wagner Yehia Mechref
author_facet	Aiying Yu Jingfu Zhao Jieqiang Zhong Junyao Wang Shiv Pratap S. Yadav Bruce A. Molitoris Mark C. Wagner Yehia Mechref
author_sort	Aiying Yu
title	Altered <i>O</i>-glycomes of Renal Brush-Border Membrane in Model Rats with Chronic Kidney Diseases
title_short	Altered <i>O</i>-glycomes of Renal Brush-Border Membrane in Model Rats with Chronic Kidney Diseases
title_full	Altered <i>O</i>-glycomes of Renal Brush-Border Membrane in Model Rats with Chronic Kidney Diseases
title_fullStr	Altered <i>O</i>-glycomes of Renal Brush-Border Membrane in Model Rats with Chronic Kidney Diseases
title_full_unstemmed	Altered <i>O</i>-glycomes of Renal Brush-Border Membrane in Model Rats with Chronic Kidney Diseases
title_sort	altered <i>o</i>-glycomes of renal brush-border membrane in model rats with chronic kidney diseases
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bbd8f5c80fb74ce9bf556ae79c00c53e
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Altered <i>O</i>-glycomes of Renal Brush-Border Membrane in Model Rats with Chronic Kidney Diseases

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