Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS

Taxilli Herba (TH) is a well-known traditional Chinese medicine (TCM) with a wide range of clinical application. However, there is a lack of comprehensive research on its chemical composition in recent years. At the same time, <i>Taxillus chinensis</i> (DC) Danser is a semi parasitic pla...

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Autores principales:	Jiahuan Yuan, Li Li, Zhichen Cai, Nan Wu, Cuihua Chen, Shengxin Yin, Shengjin Liu, Wenxin Wang, Yuqi Mei, Lifang Wei, Xunhong Liu, Lisi Zou, Haijie Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Taxilli Herba hosts chemical constituents UFLC-Triple TOF-MS/MS Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/bbda8604449a48f8ae0806a2c584bba1
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spelling	oai:doaj.org-article:bbda8604449a48f8ae0806a2c584bba12021-11-11T18:24:12ZQualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS10.3390/molecules262163731420-3049https://doaj.org/article/bbda8604449a48f8ae0806a2c584bba12021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6373https://doaj.org/toc/1420-3049Taxilli Herba (TH) is a well-known traditional Chinese medicine (TCM) with a wide range of clinical application. However, there is a lack of comprehensive research on its chemical composition in recent years. At the same time, <i>Taxillus chinensis</i> (DC) Danser is a semi parasitic plant with abundant hosts, and its chemical constituents varies due to hosts. In this study, the characterization of chemical constituents in TH was analyzed by ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (UFLC-Triple TOF-MS/MS). Moreover, partial least squares discriminant analysis (PLS-DA) was applied to reveal the differential constituents in TH from different hosts based on the qualitative information of the chemical constituents. Results showed that 73 constituents in TH were identified or tentatively presumed, including flavonoids, phenolic acids and glycosides, and others; meanwhile, the fragmentation pathways of different types of compounds were preliminarily deduced by the fragmentation behavior of the major constituents. In addition, 23 differential characteristic constituents were screened based on variable importance in projection (VIP) and <i>p</i>-value. Among them, quercetin 3-<i>O</i>-<i>β</i>-D-glucuronide, quercitrin and hyperoside were common differential constituents. Our research will contribute to comprehensive evaluation and intrinsic quality control of TH, and provide a scientific basis for the variety identification of medicinal materials from different hosts.Jiahuan YuanLi LiZhichen CaiNan WuCuihua ChenShengxin YinShengjin LiuWenxin WangYuqi MeiLifang WeiXunhong LiuLisi ZouHaijie ChenMDPI AGarticleTaxilli Herbahostschemical constituentsUFLC-Triple TOF-MS/MSOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6373, p 6373 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Taxilli Herba hosts chemical constituents UFLC-Triple TOF-MS/MS Organic chemistry QD241-441
spellingShingle	Taxilli Herba hosts chemical constituents UFLC-Triple TOF-MS/MS Organic chemistry QD241-441 Jiahuan Yuan Li Li Zhichen Cai Nan Wu Cuihua Chen Shengxin Yin Shengjin Liu Wenxin Wang Yuqi Mei Lifang Wei Xunhong Liu Lisi Zou Haijie Chen Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS
description	Taxilli Herba (TH) is a well-known traditional Chinese medicine (TCM) with a wide range of clinical application. However, there is a lack of comprehensive research on its chemical composition in recent years. At the same time, <i>Taxillus chinensis</i> (DC) Danser is a semi parasitic plant with abundant hosts, and its chemical constituents varies due to hosts. In this study, the characterization of chemical constituents in TH was analyzed by ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (UFLC-Triple TOF-MS/MS). Moreover, partial least squares discriminant analysis (PLS-DA) was applied to reveal the differential constituents in TH from different hosts based on the qualitative information of the chemical constituents. Results showed that 73 constituents in TH were identified or tentatively presumed, including flavonoids, phenolic acids and glycosides, and others; meanwhile, the fragmentation pathways of different types of compounds were preliminarily deduced by the fragmentation behavior of the major constituents. In addition, 23 differential characteristic constituents were screened based on variable importance in projection (VIP) and <i>p</i>-value. Among them, quercetin 3-<i>O</i>-<i>β</i>-D-glucuronide, quercitrin and hyperoside were common differential constituents. Our research will contribute to comprehensive evaluation and intrinsic quality control of TH, and provide a scientific basis for the variety identification of medicinal materials from different hosts.
format	article
author	Jiahuan Yuan Li Li Zhichen Cai Nan Wu Cuihua Chen Shengxin Yin Shengjin Liu Wenxin Wang Yuqi Mei Lifang Wei Xunhong Liu Lisi Zou Haijie Chen
author_facet	Jiahuan Yuan Li Li Zhichen Cai Nan Wu Cuihua Chen Shengxin Yin Shengjin Liu Wenxin Wang Yuqi Mei Lifang Wei Xunhong Liu Lisi Zou Haijie Chen
author_sort	Jiahuan Yuan
title	Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS
title_short	Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS
title_full	Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS
title_fullStr	Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS
title_full_unstemmed	Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS
title_sort	qualitative analysis and componential differences of chemical constituents in taxilli herba from different hosts by uflc-triple tof-ms/ms
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bbda8604449a48f8ae0806a2c584bba1
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Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS

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