Tobacco smoke mediated induction of sinonasal microbial biofilms.

Cigarette smokers and those exposed to second hand smoke are more susceptible to life threatening infection than non-smokers. While much is known about the devastating effect tobacco exposure has on the human body, less is known about the effect of tobacco smoke on the commensal and commonly found p...

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Autores principales: Natalia Goldstein-Daruech, Emily K Cope, Ke-Qing Zhao, Katarina Vukovic, Jennifer M Kofonow, Laurel Doghramji, Bernardo González, Alexander G Chiu, David W Kennedy, James N Palmer, Jeffery G Leid, James L Kreindler, Noam A Cohen
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Publicado: Public Library of Science (PLoS) 2011
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Acceso en línea:https://doaj.org/article/bbdedc33f8c148bd974b3a6519f23da6
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spelling oai:doaj.org-article:bbdedc33f8c148bd974b3a6519f23da62021-11-18T07:00:43ZTobacco smoke mediated induction of sinonasal microbial biofilms.1932-620310.1371/journal.pone.0015700https://doaj.org/article/bbdedc33f8c148bd974b3a6519f23da62011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21253587/?tool=EBIhttps://doaj.org/toc/1932-6203Cigarette smokers and those exposed to second hand smoke are more susceptible to life threatening infection than non-smokers. While much is known about the devastating effect tobacco exposure has on the human body, less is known about the effect of tobacco smoke on the commensal and commonly found pathogenic bacteria of the human respiratory tract, or human respiratory tract microbiome. Chronic rhinosinusitis (CRS) is a common medical complaint, affecting 16% of the US population with an estimated aggregated cost of $6 billion annually. Epidemiologic studies demonstrate a correlation between tobacco smoke exposure and rhinosinusitis. Although a common cause of CRS has not been defined, bacterial presence within the nasal and paranasal sinuses is assumed to be contributory. Here we demonstrate that repetitive tobacco smoke exposure induces biofilm formation in a diverse set of bacteria isolated from the sinonasal cavities of patients with CRS. Additionally, bacteria isolated from patients with tobacco smoke exposure demonstrate robust in vitro biofilm formation when challenged with tobacco smoke compared to those isolated from smoke naïve patients. Lastly, bacteria from smoke exposed patients can revert to a non-biofilm phenotype when grown in the absence of tobacco smoke. These observations support the hypothesis that tobacco exposure induces sinonasal biofilm formation, thereby contributing to the conversion of a transient and medically treatable infection to a persistent and therapeutically recalcitrant condition.Natalia Goldstein-DaruechEmily K CopeKe-Qing ZhaoKatarina VukovicJennifer M KofonowLaurel DoghramjiBernardo GonzálezAlexander G ChiuDavid W KennedyJames N PalmerJeffery G LeidJames L KreindlerNoam A CohenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e15700 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Natalia Goldstein-Daruech
Emily K Cope
Ke-Qing Zhao
Katarina Vukovic
Jennifer M Kofonow
Laurel Doghramji
Bernardo González
Alexander G Chiu
David W Kennedy
James N Palmer
Jeffery G Leid
James L Kreindler
Noam A Cohen
Tobacco smoke mediated induction of sinonasal microbial biofilms.
description Cigarette smokers and those exposed to second hand smoke are more susceptible to life threatening infection than non-smokers. While much is known about the devastating effect tobacco exposure has on the human body, less is known about the effect of tobacco smoke on the commensal and commonly found pathogenic bacteria of the human respiratory tract, or human respiratory tract microbiome. Chronic rhinosinusitis (CRS) is a common medical complaint, affecting 16% of the US population with an estimated aggregated cost of $6 billion annually. Epidemiologic studies demonstrate a correlation between tobacco smoke exposure and rhinosinusitis. Although a common cause of CRS has not been defined, bacterial presence within the nasal and paranasal sinuses is assumed to be contributory. Here we demonstrate that repetitive tobacco smoke exposure induces biofilm formation in a diverse set of bacteria isolated from the sinonasal cavities of patients with CRS. Additionally, bacteria isolated from patients with tobacco smoke exposure demonstrate robust in vitro biofilm formation when challenged with tobacco smoke compared to those isolated from smoke naïve patients. Lastly, bacteria from smoke exposed patients can revert to a non-biofilm phenotype when grown in the absence of tobacco smoke. These observations support the hypothesis that tobacco exposure induces sinonasal biofilm formation, thereby contributing to the conversion of a transient and medically treatable infection to a persistent and therapeutically recalcitrant condition.
format article
author Natalia Goldstein-Daruech
Emily K Cope
Ke-Qing Zhao
Katarina Vukovic
Jennifer M Kofonow
Laurel Doghramji
Bernardo González
Alexander G Chiu
David W Kennedy
James N Palmer
Jeffery G Leid
James L Kreindler
Noam A Cohen
author_facet Natalia Goldstein-Daruech
Emily K Cope
Ke-Qing Zhao
Katarina Vukovic
Jennifer M Kofonow
Laurel Doghramji
Bernardo González
Alexander G Chiu
David W Kennedy
James N Palmer
Jeffery G Leid
James L Kreindler
Noam A Cohen
author_sort Natalia Goldstein-Daruech
title Tobacco smoke mediated induction of sinonasal microbial biofilms.
title_short Tobacco smoke mediated induction of sinonasal microbial biofilms.
title_full Tobacco smoke mediated induction of sinonasal microbial biofilms.
title_fullStr Tobacco smoke mediated induction of sinonasal microbial biofilms.
title_full_unstemmed Tobacco smoke mediated induction of sinonasal microbial biofilms.
title_sort tobacco smoke mediated induction of sinonasal microbial biofilms.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/bbdedc33f8c148bd974b3a6519f23da6
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