The Correlate between Argyrophilic Nucleolar Organizer Region Stain and Ki-67 Immnochistochemistry in Diagnostic Breast Cancer

Introduction: This experimental study gauged the value of argyrophilic nucleolar organizer region (AgNOR) staining as a possible technique for the estimation of cell kinetics in conventional histology sections, in benign and malignant breast lesions. Methods: With a silver staining technique and im...

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Autores principales:	Hassan ALgarsh, Hana Abusaida, Fairouz Torjman
Formato:	article
Lenguaje:	EN
Publicado:	Emergency Department of Hospital San Pedro (Logroño, Spain) 2020
Materias:	breast cancer agnors ki67 benign malignant Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bbe40fa5b3ad408281d372e8d7a12b93
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spelling	oai:doaj.org-article:bbe40fa5b3ad408281d372e8d7a12b932021-12-02T18:12:17ZThe Correlate between Argyrophilic Nucleolar Organizer Region Stain and Ki-67 Immnochistochemistry in Diagnostic Breast Cancer10.5281/zenodo.40437762695-5075https://doaj.org/article/bbe40fa5b3ad408281d372e8d7a12b932020-09-01T00:00:00Zhttps://doi.org/10.5281/zenodo.4043776https://doaj.org/toc/2695-5075Introduction: This experimental study gauged the value of argyrophilic nucleolar organizer region (AgNOR) staining as a possible technique for the estimation of cell kinetics in conventional histology sections, in benign and malignant breast lesions. Methods: With a silver staining technique and immunohistochemistry, we associated the numbers of AgNORs and Ki67 scores in 30 breast carcinomas and 10 benign breast lesions. Results: The mean values of Ag NORs silver stain dots count for normal, benign, grade II and III were 1.28±0.17, 2.83±0.68, 5.23±0.87 and 7.32±0.92, respectively. There was a statistically significant difference (p≤0.001) was noticed between the all individual groups, among the normal and breast lesion as well as among the GII and GIII. Immunohistochemical Results of Ki-67 protein exhibited homogenous golden-brown color in control case and a positive brown granules or diffuse dark brown color in the nuclei of both benign and malignant cases under the 400X magnify examined under the light microscope. Discussion: AgNOR counts performed on routine formalin-fixed paraffin sections could provide substantial kinetic evidence. Additionally, the difference in AgNOR counts between benign and malignant tumors is such that they may be of diagnostic worth.Hassan ALgarshHana AbusaidaFairouz TorjmanEmergency Department of Hospital San Pedro (Logroño, Spain)articlebreast canceragnorski67benignmalignantMedicine (General)R5-920ENIberoamerican Journal of Medicine, Vol 2, Iss 4, Pp 285-292 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast cancer agnors ki67 benign malignant Medicine (General) R5-920
spellingShingle	breast cancer agnors ki67 benign malignant Medicine (General) R5-920 Hassan ALgarsh Hana Abusaida Fairouz Torjman The Correlate between Argyrophilic Nucleolar Organizer Region Stain and Ki-67 Immnochistochemistry in Diagnostic Breast Cancer
description	Introduction: This experimental study gauged the value of argyrophilic nucleolar organizer region (AgNOR) staining as a possible technique for the estimation of cell kinetics in conventional histology sections, in benign and malignant breast lesions. Methods: With a silver staining technique and immunohistochemistry, we associated the numbers of AgNORs and Ki67 scores in 30 breast carcinomas and 10 benign breast lesions. Results: The mean values of Ag NORs silver stain dots count for normal, benign, grade II and III were 1.28±0.17, 2.83±0.68, 5.23±0.87 and 7.32±0.92, respectively. There was a statistically significant difference (p≤0.001) was noticed between the all individual groups, among the normal and breast lesion as well as among the GII and GIII. Immunohistochemical Results of Ki-67 protein exhibited homogenous golden-brown color in control case and a positive brown granules or diffuse dark brown color in the nuclei of both benign and malignant cases under the 400X magnify examined under the light microscope. Discussion: AgNOR counts performed on routine formalin-fixed paraffin sections could provide substantial kinetic evidence. Additionally, the difference in AgNOR counts between benign and malignant tumors is such that they may be of diagnostic worth.
format	article
author	Hassan ALgarsh Hana Abusaida Fairouz Torjman
author_facet	Hassan ALgarsh Hana Abusaida Fairouz Torjman
author_sort	Hassan ALgarsh
title	The Correlate between Argyrophilic Nucleolar Organizer Region Stain and Ki-67 Immnochistochemistry in Diagnostic Breast Cancer
title_short	The Correlate between Argyrophilic Nucleolar Organizer Region Stain and Ki-67 Immnochistochemistry in Diagnostic Breast Cancer
title_full	The Correlate between Argyrophilic Nucleolar Organizer Region Stain and Ki-67 Immnochistochemistry in Diagnostic Breast Cancer
title_fullStr	The Correlate between Argyrophilic Nucleolar Organizer Region Stain and Ki-67 Immnochistochemistry in Diagnostic Breast Cancer
title_full_unstemmed	The Correlate between Argyrophilic Nucleolar Organizer Region Stain and Ki-67 Immnochistochemistry in Diagnostic Breast Cancer
title_sort	correlate between argyrophilic nucleolar organizer region stain and ki-67 immnochistochemistry in diagnostic breast cancer
publisher	Emergency Department of Hospital San Pedro (Logroño, Spain)
publishDate	2020
url	https://doaj.org/article/bbe40fa5b3ad408281d372e8d7a12b93
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The Correlate between Argyrophilic Nucleolar Organizer Region Stain and Ki-67 Immnochistochemistry in Diagnostic Breast Cancer

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