Transcriptomic analysis of the black tiger shrimp (Penaeus monodon) reveals insights into immune development in their early life stages

Abstract With the rapid growth in the global demand, the shrimp industry needs integrated approaches for sustainable production. A high-quality shrimp larva is one of the crucial key requirements to maximize shrimp production. Survival and growth rates during larval development are often criteria to...

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Autores principales:	Pacharaporn Angthong, Tanaporn Uengwetwanit, Sopacha Arayamethakorn, Wanilada Rungrassamee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bbefe952dd2a4d16a727643f9160fade
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spelling	oai:doaj.org-article:bbefe952dd2a4d16a727643f9160fade2021-12-02T18:34:07ZTranscriptomic analysis of the black tiger shrimp (Penaeus monodon) reveals insights into immune development in their early life stages10.1038/s41598-021-93364-92045-2322https://doaj.org/article/bbefe952dd2a4d16a727643f9160fade2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93364-9https://doaj.org/toc/2045-2322Abstract With the rapid growth in the global demand, the shrimp industry needs integrated approaches for sustainable production. A high-quality shrimp larva is one of the crucial key requirements to maximize shrimp production. Survival and growth rates during larval development are often criteria to evaluate larval quality, however many aspects of gene regulation during shrimp larval development have not yet been identified. To further our understanding of biological processes in their early life, transcriptomic analysis of larval developmental stages (nauplius, zoea, mysis, and postlarva) were determined in the black tiger shrimp, Penaeus monodon using next-generation RNA sequencing. Gene clustering and gene enrichment analyses revealed that most of the transcripts were mainly related to metabolic processes, cell and growth development, and immune system. Interestingly, Spätzle and Toll receptors were found in nauplius stage, providing evidence that Toll pathway was a baseline immune system established in early larval stages. Genes encoding pathogen pattern-recognition proteins (LGBP, PL5-2 and c-type lectin), prophenoloxidase system (PPAE2, PPAF2 and serpin), antimicrobial peptides (crustin and antiviral protein), blood clotting system (hemolymph clottable protein) and heat shock protein (HSP70) were expressed as they developed further, suggesting that these immune defense mechanisms were established in later larval stages.Pacharaporn AngthongTanaporn UengwetwanitSopacha ArayamethakornWanilada RungrassameeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pacharaporn Angthong Tanaporn Uengwetwanit Sopacha Arayamethakorn Wanilada Rungrassamee Transcriptomic analysis of the black tiger shrimp (Penaeus monodon) reveals insights into immune development in their early life stages
description	Abstract With the rapid growth in the global demand, the shrimp industry needs integrated approaches for sustainable production. A high-quality shrimp larva is one of the crucial key requirements to maximize shrimp production. Survival and growth rates during larval development are often criteria to evaluate larval quality, however many aspects of gene regulation during shrimp larval development have not yet been identified. To further our understanding of biological processes in their early life, transcriptomic analysis of larval developmental stages (nauplius, zoea, mysis, and postlarva) were determined in the black tiger shrimp, Penaeus monodon using next-generation RNA sequencing. Gene clustering and gene enrichment analyses revealed that most of the transcripts were mainly related to metabolic processes, cell and growth development, and immune system. Interestingly, Spätzle and Toll receptors were found in nauplius stage, providing evidence that Toll pathway was a baseline immune system established in early larval stages. Genes encoding pathogen pattern-recognition proteins (LGBP, PL5-2 and c-type lectin), prophenoloxidase system (PPAE2, PPAF2 and serpin), antimicrobial peptides (crustin and antiviral protein), blood clotting system (hemolymph clottable protein) and heat shock protein (HSP70) were expressed as they developed further, suggesting that these immune defense mechanisms were established in later larval stages.
format	article
author	Pacharaporn Angthong Tanaporn Uengwetwanit Sopacha Arayamethakorn Wanilada Rungrassamee
author_facet	Pacharaporn Angthong Tanaporn Uengwetwanit Sopacha Arayamethakorn Wanilada Rungrassamee
author_sort	Pacharaporn Angthong
title	Transcriptomic analysis of the black tiger shrimp (Penaeus monodon) reveals insights into immune development in their early life stages
title_short	Transcriptomic analysis of the black tiger shrimp (Penaeus monodon) reveals insights into immune development in their early life stages
title_full	Transcriptomic analysis of the black tiger shrimp (Penaeus monodon) reveals insights into immune development in their early life stages
title_fullStr	Transcriptomic analysis of the black tiger shrimp (Penaeus monodon) reveals insights into immune development in their early life stages
title_full_unstemmed	Transcriptomic analysis of the black tiger shrimp (Penaeus monodon) reveals insights into immune development in their early life stages
title_sort	transcriptomic analysis of the black tiger shrimp (penaeus monodon) reveals insights into immune development in their early life stages
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bbefe952dd2a4d16a727643f9160fade
work_keys_str_mv	AT pacharapornangthong transcriptomicanalysisoftheblacktigershrimppenaeusmonodonrevealsinsightsintoimmunedevelopmentintheirearlylifestages AT tanapornuengwetwanit transcriptomicanalysisoftheblacktigershrimppenaeusmonodonrevealsinsightsintoimmunedevelopmentintheirearlylifestages AT sopachaarayamethakorn transcriptomicanalysisoftheblacktigershrimppenaeusmonodonrevealsinsightsintoimmunedevelopmentintheirearlylifestages AT waniladarungrassamee transcriptomicanalysisoftheblacktigershrimppenaeusmonodonrevealsinsightsintoimmunedevelopmentintheirearlylifestages
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Transcriptomic analysis of the black tiger shrimp (Penaeus monodon) reveals insights into immune development in their early life stages

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