Immunohistochemical Evaluation of Adaptor Protein FAM159B Expression in Normal and Neoplastic Human Tissues

FAM159B is a so-called adaptor protein. These proteins are essential components in numerous cell signalling pathways. However, little is known regarding FAM159B expression in normal and neoplastic human tissues. The commercially available rabbit polyclonal anti-human FAM159B antibody HPA011778 was i...

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Autores principales:	Anna-Sophia Lieselott Beyer, Daniel Kaemmerer, Jörg Sänger, Katja Evert, Amelie Lupp
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	FAM159B adaptor protein antibody immunohistochemistry neuroendocrine tumour Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/bbf11c845a8242f294b1bd619431ac29
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spelling	oai:doaj.org-article:bbf11c845a8242f294b1bd619431ac292021-11-25T17:54:47ZImmunohistochemical Evaluation of Adaptor Protein FAM159B Expression in Normal and Neoplastic Human Tissues10.3390/ijms2222122501422-00671661-6596https://doaj.org/article/bbf11c845a8242f294b1bd619431ac292021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12250https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067FAM159B is a so-called adaptor protein. These proteins are essential components in numerous cell signalling pathways. However, little is known regarding FAM159B expression in normal and neoplastic human tissues. The commercially available rabbit polyclonal anti-human FAM159B antibody HPA011778 was initially characterised for its specificity using Western blot analyses and immunocytochemistry and then applied to a large series of formalin-fixed, paraffin-embedded normal and neoplastic human tissue samples. Confirmation of FAM159B’s predicted size and antibody specificity was achieved in BON-1 cells, a neuroendocrine tumour cell line endogenously expressing FAM159B, using targeted siRNA. Immunocytochemical experiments additionally revealed cytoplasmic expression of the adaptor protein. Immunohistochemical staining detected FAM159B expression in neuronal and neuroendocrine tissues such as the cortex, the trigeminal ganglia, dorsal root and intestinal ganglia, the pancreatic islets and the neuroendocrine cells of the bronchopulmonary and gastrointestinal tract, but also in the syncytiotrophoblasts of the placenta. FAM159B was also expressed in many of the 28 tumour entities investigated, with high levels in medullary and anaplastic thyroid carcinomas, parathyroid adenomas, lung and ovarian carcinomas, lymphomas and neuroendocrine tumours of different origins. The antibody HPA011778 can act as a useful tool for basic research and identifying FAM159B expression in tissue samples.Anna-Sophia Lieselott BeyerDaniel KaemmererJörg SängerKatja EvertAmelie LuppMDPI AGarticleFAM159Badaptor proteinantibodyimmunohistochemistryneuroendocrinetumourBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12250, p 12250 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	FAM159B adaptor protein antibody immunohistochemistry neuroendocrine tumour Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	FAM159B adaptor protein antibody immunohistochemistry neuroendocrine tumour Biology (General) QH301-705.5 Chemistry QD1-999 Anna-Sophia Lieselott Beyer Daniel Kaemmerer Jörg Sänger Katja Evert Amelie Lupp Immunohistochemical Evaluation of Adaptor Protein FAM159B Expression in Normal and Neoplastic Human Tissues
description	FAM159B is a so-called adaptor protein. These proteins are essential components in numerous cell signalling pathways. However, little is known regarding FAM159B expression in normal and neoplastic human tissues. The commercially available rabbit polyclonal anti-human FAM159B antibody HPA011778 was initially characterised for its specificity using Western blot analyses and immunocytochemistry and then applied to a large series of formalin-fixed, paraffin-embedded normal and neoplastic human tissue samples. Confirmation of FAM159B’s predicted size and antibody specificity was achieved in BON-1 cells, a neuroendocrine tumour cell line endogenously expressing FAM159B, using targeted siRNA. Immunocytochemical experiments additionally revealed cytoplasmic expression of the adaptor protein. Immunohistochemical staining detected FAM159B expression in neuronal and neuroendocrine tissues such as the cortex, the trigeminal ganglia, dorsal root and intestinal ganglia, the pancreatic islets and the neuroendocrine cells of the bronchopulmonary and gastrointestinal tract, but also in the syncytiotrophoblasts of the placenta. FAM159B was also expressed in many of the 28 tumour entities investigated, with high levels in medullary and anaplastic thyroid carcinomas, parathyroid adenomas, lung and ovarian carcinomas, lymphomas and neuroendocrine tumours of different origins. The antibody HPA011778 can act as a useful tool for basic research and identifying FAM159B expression in tissue samples.
format	article
author	Anna-Sophia Lieselott Beyer Daniel Kaemmerer Jörg Sänger Katja Evert Amelie Lupp
author_facet	Anna-Sophia Lieselott Beyer Daniel Kaemmerer Jörg Sänger Katja Evert Amelie Lupp
author_sort	Anna-Sophia Lieselott Beyer
title	Immunohistochemical Evaluation of Adaptor Protein FAM159B Expression in Normal and Neoplastic Human Tissues
title_short	Immunohistochemical Evaluation of Adaptor Protein FAM159B Expression in Normal and Neoplastic Human Tissues
title_full	Immunohistochemical Evaluation of Adaptor Protein FAM159B Expression in Normal and Neoplastic Human Tissues
title_fullStr	Immunohistochemical Evaluation of Adaptor Protein FAM159B Expression in Normal and Neoplastic Human Tissues
title_full_unstemmed	Immunohistochemical Evaluation of Adaptor Protein FAM159B Expression in Normal and Neoplastic Human Tissues
title_sort	immunohistochemical evaluation of adaptor protein fam159b expression in normal and neoplastic human tissues
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bbf11c845a8242f294b1bd619431ac29
work_keys_str_mv	AT annasophialieselottbeyer immunohistochemicalevaluationofadaptorproteinfam159bexpressioninnormalandneoplastichumantissues AT danielkaemmerer immunohistochemicalevaluationofadaptorproteinfam159bexpressioninnormalandneoplastichumantissues AT jorgsanger immunohistochemicalevaluationofadaptorproteinfam159bexpressioninnormalandneoplastichumantissues AT katjaevert immunohistochemicalevaluationofadaptorproteinfam159bexpressioninnormalandneoplastichumantissues AT amelielupp immunohistochemicalevaluationofadaptorproteinfam159bexpressioninnormalandneoplastichumantissues
_version_	1718411884253675520

Immunohistochemical Evaluation of Adaptor Protein FAM159B Expression in Normal and Neoplastic Human Tissues

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