Role of actin filaments in correlating nuclear shape and cell spreading.

It is well known that substrate properties like stiffness and adhesivity influence stem cell morphology and differentiation. Recent experiments show that cell morphology influences nuclear geometry and hence gene expression profile. The mechanism by which surface properties regulate cell and nuclear...

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Autores principales:	Renu Vishavkarma, Swetavalli Raghavan, Chandrashekar Kuyyamudi, Abhijit Majumder, Jyotsna Dhawan, Pramod A Pullarkat
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc0078c3306849398ec99ad924806599
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spelling	oai:doaj.org-article:bc0078c3306849398ec99ad9248065992021-11-25T05:59:30ZRole of actin filaments in correlating nuclear shape and cell spreading.1932-620310.1371/journal.pone.0107895https://doaj.org/article/bc0078c3306849398ec99ad9248065992014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107895https://doaj.org/toc/1932-6203It is well known that substrate properties like stiffness and adhesivity influence stem cell morphology and differentiation. Recent experiments show that cell morphology influences nuclear geometry and hence gene expression profile. The mechanism by which surface properties regulate cell and nuclear properties is only beginning to be understood. Direct transmission of forces as well as chemical signalling are involved in this process. Here, we investigate the formal aspect by studying the correlation between cell spreading and nuclear deformation using Mesenchymal stem cells under a wide variety of conditions. It is observed that a robust quantitative relation holds between the cell and nuclear projected areas, irrespective of how the cell area is modified or when various cytoskeletal or nuclear components are perturbed. By studying the role of actin stress fibers in compressing the nucleus we propose that nuclear compression by stress fibers can lead to enhanced cell spreading due to an interplay between elastic and adhesion factors. The significance of myosin-II in regulating this process is also explored. We demonstrate this effect using a simple technique to apply external compressive loads on the nucleus.Renu VishavkarmaSwetavalli RaghavanChandrashekar KuyyamudiAbhijit MajumderJyotsna DhawanPramod A PullarkatPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107895 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Renu Vishavkarma Swetavalli Raghavan Chandrashekar Kuyyamudi Abhijit Majumder Jyotsna Dhawan Pramod A Pullarkat Role of actin filaments in correlating nuclear shape and cell spreading.
description	It is well known that substrate properties like stiffness and adhesivity influence stem cell morphology and differentiation. Recent experiments show that cell morphology influences nuclear geometry and hence gene expression profile. The mechanism by which surface properties regulate cell and nuclear properties is only beginning to be understood. Direct transmission of forces as well as chemical signalling are involved in this process. Here, we investigate the formal aspect by studying the correlation between cell spreading and nuclear deformation using Mesenchymal stem cells under a wide variety of conditions. It is observed that a robust quantitative relation holds between the cell and nuclear projected areas, irrespective of how the cell area is modified or when various cytoskeletal or nuclear components are perturbed. By studying the role of actin stress fibers in compressing the nucleus we propose that nuclear compression by stress fibers can lead to enhanced cell spreading due to an interplay between elastic and adhesion factors. The significance of myosin-II in regulating this process is also explored. We demonstrate this effect using a simple technique to apply external compressive loads on the nucleus.
format	article
author	Renu Vishavkarma Swetavalli Raghavan Chandrashekar Kuyyamudi Abhijit Majumder Jyotsna Dhawan Pramod A Pullarkat
author_facet	Renu Vishavkarma Swetavalli Raghavan Chandrashekar Kuyyamudi Abhijit Majumder Jyotsna Dhawan Pramod A Pullarkat
author_sort	Renu Vishavkarma
title	Role of actin filaments in correlating nuclear shape and cell spreading.
title_short	Role of actin filaments in correlating nuclear shape and cell spreading.
title_full	Role of actin filaments in correlating nuclear shape and cell spreading.
title_fullStr	Role of actin filaments in correlating nuclear shape and cell spreading.
title_full_unstemmed	Role of actin filaments in correlating nuclear shape and cell spreading.
title_sort	role of actin filaments in correlating nuclear shape and cell spreading.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/bc0078c3306849398ec99ad924806599
work_keys_str_mv	AT renuvishavkarma roleofactinfilamentsincorrelatingnuclearshapeandcellspreading AT swetavalliraghavan roleofactinfilamentsincorrelatingnuclearshapeandcellspreading AT chandrashekarkuyyamudi roleofactinfilamentsincorrelatingnuclearshapeandcellspreading AT abhijitmajumder roleofactinfilamentsincorrelatingnuclearshapeandcellspreading AT jyotsnadhawan roleofactinfilamentsincorrelatingnuclearshapeandcellspreading AT pramodapullarkat roleofactinfilamentsincorrelatingnuclearshapeandcellspreading
_version_	1718414303449579520

Role of actin filaments in correlating nuclear shape and cell spreading.

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