Unconventional conservation reveals structure-function relationships in the synaptonemal complex

Functional requirements constrain protein evolution, commonly manifesting in a conserved amino acid sequence. Here, we extend this idea to secondary structural features by tracking their conservation in essential meiotic proteins with highly diverged sequences. The synaptonemal complex (SC) is a ~10...

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Autores principales:	Lisa E Kursel, Henry D Cope, Ofer Rog
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	P. pacificus meiosis synaptonemal complex coiled-coil indel evolution Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bc16d92a8a5a42d0b4692e96904a7b5b
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spelling	oai:doaj.org-article:bc16d92a8a5a42d0b4692e96904a7b5b2021-11-24T12:24:08ZUnconventional conservation reveals structure-function relationships in the synaptonemal complex10.7554/eLife.720612050-084Xe72061https://doaj.org/article/bc16d92a8a5a42d0b4692e96904a7b5b2021-11-01T00:00:00Zhttps://elifesciences.org/articles/72061https://doaj.org/toc/2050-084XFunctional requirements constrain protein evolution, commonly manifesting in a conserved amino acid sequence. Here, we extend this idea to secondary structural features by tracking their conservation in essential meiotic proteins with highly diverged sequences. The synaptonemal complex (SC) is a ~100-nm-wide ladder-like meiotic structure present in all eukaryotic clades, where it aligns parental chromosomes and regulates exchanges between them. Despite the conserved ultrastructure and functions of the SC, SC proteins are highly divergent within Caenorhabditis. However, SC proteins have highly conserved length and coiled-coil domain structure. We found the same unconventional conservation signature in Drosophila and mammals, and used it to identify a novel SC protein in Pristionchus pacificus, Ppa-SYP-1. Our work suggests that coiled-coils play wide-ranging roles in the structure and function of the SC, and more broadly, that expanding sequence analysis beyond measures of per-site similarity can enhance our understanding of protein evolution and function.Lisa E KurselHenry D CopeOfer RogeLife Sciences Publications LtdarticleP. pacificusmeiosissynaptonemal complexcoiled-coilindelevolutionMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	P. pacificus meiosis synaptonemal complex coiled-coil indel evolution Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	P. pacificus meiosis synaptonemal complex coiled-coil indel evolution Medicine R Science Q Biology (General) QH301-705.5 Lisa E Kursel Henry D Cope Ofer Rog Unconventional conservation reveals structure-function relationships in the synaptonemal complex
description	Functional requirements constrain protein evolution, commonly manifesting in a conserved amino acid sequence. Here, we extend this idea to secondary structural features by tracking their conservation in essential meiotic proteins with highly diverged sequences. The synaptonemal complex (SC) is a ~100-nm-wide ladder-like meiotic structure present in all eukaryotic clades, where it aligns parental chromosomes and regulates exchanges between them. Despite the conserved ultrastructure and functions of the SC, SC proteins are highly divergent within Caenorhabditis. However, SC proteins have highly conserved length and coiled-coil domain structure. We found the same unconventional conservation signature in Drosophila and mammals, and used it to identify a novel SC protein in Pristionchus pacificus, Ppa-SYP-1. Our work suggests that coiled-coils play wide-ranging roles in the structure and function of the SC, and more broadly, that expanding sequence analysis beyond measures of per-site similarity can enhance our understanding of protein evolution and function.
format	article
author	Lisa E Kursel Henry D Cope Ofer Rog
author_facet	Lisa E Kursel Henry D Cope Ofer Rog
author_sort	Lisa E Kursel
title	Unconventional conservation reveals structure-function relationships in the synaptonemal complex
title_short	Unconventional conservation reveals structure-function relationships in the synaptonemal complex
title_full	Unconventional conservation reveals structure-function relationships in the synaptonemal complex
title_fullStr	Unconventional conservation reveals structure-function relationships in the synaptonemal complex
title_full_unstemmed	Unconventional conservation reveals structure-function relationships in the synaptonemal complex
title_sort	unconventional conservation reveals structure-function relationships in the synaptonemal complex
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/bc16d92a8a5a42d0b4692e96904a7b5b
work_keys_str_mv	AT lisaekursel unconventionalconservationrevealsstructurefunctionrelationshipsinthesynaptonemalcomplex AT henrydcope unconventionalconservationrevealsstructurefunctionrelationshipsinthesynaptonemalcomplex AT oferrog unconventionalconservationrevealsstructurefunctionrelationshipsinthesynaptonemalcomplex
_version_	1718415071735971840

Unconventional conservation reveals structure-function relationships in the synaptonemal complex

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