HLA-E Presents Glycopeptides from the Mycobacterium tuberculosis Protein MPT32 to Human CD8+ T cells

Abstract Infection with Mycobacterium tuberculosis (Mtb), the bacterium that causes tuberculosis, remains a global health concern. Both classically and non-classically restricted cytotoxic CD8+ T cells are important to the control of Mtb infection. We and others have demonstrated that the non-classi...

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Autores principales:	Melanie J. Harriff, Lisa M. Wolfe, Gwendolyn Swarbrick, Megan Null, Meghan E. Cansler, Elizabeth T. Canfield, Todd Vogt, Katelynne Gardner Toren, Wei Li, Mary Jackson, Deborah A. Lewinsohn, Karen M. Dobos, David M. Lewinsohn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc18a49d1954428b9bb82c6c9deb9699
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spelling	oai:doaj.org-article:bc18a49d1954428b9bb82c6c9deb96992021-12-02T15:05:05ZHLA-E Presents Glycopeptides from the Mycobacterium tuberculosis Protein MPT32 to Human CD8+ T cells10.1038/s41598-017-04894-02045-2322https://doaj.org/article/bc18a49d1954428b9bb82c6c9deb96992017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04894-0https://doaj.org/toc/2045-2322Abstract Infection with Mycobacterium tuberculosis (Mtb), the bacterium that causes tuberculosis, remains a global health concern. Both classically and non-classically restricted cytotoxic CD8+ T cells are important to the control of Mtb infection. We and others have demonstrated that the non-classical MHC I molecule HLA-E can present pathogen-derived peptides to CD8+ T cells. In this manuscript, we identified the antigen recognized by an HLA-E-restricted CD8+ T cell clone isolated from an Mtb latently infected individual as a peptide from the Mtb protein, MPT32. Recognition by the CD8+ T cell clone required N-terminal O-linked mannosylation of MPT32 by a mannosyltransferase encoded by the Rv1002c gene. This is the first description of a post-translationally modified Mtb-derived protein antigen presented in the context of an HLA-E specific CD8+ T cell immune response. The identification of an immune response that targets a unique mycobacterial modification is novel and may have practical impact in the development of vaccines and diagnostics.Melanie J. HarriffLisa M. WolfeGwendolyn SwarbrickMegan NullMeghan E. CanslerElizabeth T. CanfieldTodd VogtKatelynne Gardner TorenWei LiMary JacksonDeborah A. LewinsohnKaren M. DobosDavid M. LewinsohnNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Melanie J. Harriff Lisa M. Wolfe Gwendolyn Swarbrick Megan Null Meghan E. Cansler Elizabeth T. Canfield Todd Vogt Katelynne Gardner Toren Wei Li Mary Jackson Deborah A. Lewinsohn Karen M. Dobos David M. Lewinsohn HLA-E Presents Glycopeptides from the Mycobacterium tuberculosis Protein MPT32 to Human CD8+ T cells
description	Abstract Infection with Mycobacterium tuberculosis (Mtb), the bacterium that causes tuberculosis, remains a global health concern. Both classically and non-classically restricted cytotoxic CD8+ T cells are important to the control of Mtb infection. We and others have demonstrated that the non-classical MHC I molecule HLA-E can present pathogen-derived peptides to CD8+ T cells. In this manuscript, we identified the antigen recognized by an HLA-E-restricted CD8+ T cell clone isolated from an Mtb latently infected individual as a peptide from the Mtb protein, MPT32. Recognition by the CD8+ T cell clone required N-terminal O-linked mannosylation of MPT32 by a mannosyltransferase encoded by the Rv1002c gene. This is the first description of a post-translationally modified Mtb-derived protein antigen presented in the context of an HLA-E specific CD8+ T cell immune response. The identification of an immune response that targets a unique mycobacterial modification is novel and may have practical impact in the development of vaccines and diagnostics.
format	article
author	Melanie J. Harriff Lisa M. Wolfe Gwendolyn Swarbrick Megan Null Meghan E. Cansler Elizabeth T. Canfield Todd Vogt Katelynne Gardner Toren Wei Li Mary Jackson Deborah A. Lewinsohn Karen M. Dobos David M. Lewinsohn
author_facet	Melanie J. Harriff Lisa M. Wolfe Gwendolyn Swarbrick Megan Null Meghan E. Cansler Elizabeth T. Canfield Todd Vogt Katelynne Gardner Toren Wei Li Mary Jackson Deborah A. Lewinsohn Karen M. Dobos David M. Lewinsohn
author_sort	Melanie J. Harriff
title	HLA-E Presents Glycopeptides from the Mycobacterium tuberculosis Protein MPT32 to Human CD8+ T cells
title_short	HLA-E Presents Glycopeptides from the Mycobacterium tuberculosis Protein MPT32 to Human CD8+ T cells
title_full	HLA-E Presents Glycopeptides from the Mycobacterium tuberculosis Protein MPT32 to Human CD8+ T cells
title_fullStr	HLA-E Presents Glycopeptides from the Mycobacterium tuberculosis Protein MPT32 to Human CD8+ T cells
title_full_unstemmed	HLA-E Presents Glycopeptides from the Mycobacterium tuberculosis Protein MPT32 to Human CD8+ T cells
title_sort	hla-e presents glycopeptides from the mycobacterium tuberculosis protein mpt32 to human cd8+ t cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bc18a49d1954428b9bb82c6c9deb9699
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HLA-E Presents Glycopeptides from the Mycobacterium tuberculosis Protein MPT32 to Human CD8+ T cells

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