Physiological and genetic characterization of calcium phosphate precipitation by Pseudomonas species

Abstract Microbial biomineralization is a widespread phenomenon. The ability to induce calcium precipitation around bacterial cells has been reported in several Pseudomonas species but has not been thoroughly tested. We assayed 14 Pseudomonas strains representing five different species for the abili...

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Autores principales:	Maxwell R. Fishman, Krista Giglio, David Fay, Melanie J. Filiatrault
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc1ccfaa8f6f45e0a1d0e83df7490092
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spelling	oai:doaj.org-article:bc1ccfaa8f6f45e0a1d0e83df74900922021-12-02T15:08:17ZPhysiological and genetic characterization of calcium phosphate precipitation by Pseudomonas species10.1038/s41598-018-28525-42045-2322https://doaj.org/article/bc1ccfaa8f6f45e0a1d0e83df74900922018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-28525-4https://doaj.org/toc/2045-2322Abstract Microbial biomineralization is a widespread phenomenon. The ability to induce calcium precipitation around bacterial cells has been reported in several Pseudomonas species but has not been thoroughly tested. We assayed 14 Pseudomonas strains representing five different species for the ability to precipitate calcium. Calcium phosphate precipitated adjacent to the colonies of all the Pseudomonas strains tested and also precipitated on the surface of colonies for several of the Pseudomonas strains assayed. The precipitate was commonly precipitated as amorphous calcium phosphate, however seven of the 14 Pseudomonas strains tested precipitated amorphous apatite in agar adjacent to the colonies. Out of the seven Pseudomonas strains that precipitated amorphous apatite, six are plant pathogenic. The formation of amorphous apatite was commonly observed in the area of the agar where amorphous calcium phosphate had previously formed. A transposon mutagenesis screen in Pseudomonas syringae pv. tomato DC3000 revealed genes involved in general metabolism, lipopolysaccharide and cell wall biogenesis, and in regulation of virulence play a role in calcium precipitation. These results shed light on the common ability of Pseudomonas species to perform calcium precipitation and the underlying genetic regulation involved in biomineralization.Maxwell R. FishmanKrista GiglioDavid FayMelanie J. FiliatraultNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maxwell R. Fishman Krista Giglio David Fay Melanie J. Filiatrault Physiological and genetic characterization of calcium phosphate precipitation by Pseudomonas species
description	Abstract Microbial biomineralization is a widespread phenomenon. The ability to induce calcium precipitation around bacterial cells has been reported in several Pseudomonas species but has not been thoroughly tested. We assayed 14 Pseudomonas strains representing five different species for the ability to precipitate calcium. Calcium phosphate precipitated adjacent to the colonies of all the Pseudomonas strains tested and also precipitated on the surface of colonies for several of the Pseudomonas strains assayed. The precipitate was commonly precipitated as amorphous calcium phosphate, however seven of the 14 Pseudomonas strains tested precipitated amorphous apatite in agar adjacent to the colonies. Out of the seven Pseudomonas strains that precipitated amorphous apatite, six are plant pathogenic. The formation of amorphous apatite was commonly observed in the area of the agar where amorphous calcium phosphate had previously formed. A transposon mutagenesis screen in Pseudomonas syringae pv. tomato DC3000 revealed genes involved in general metabolism, lipopolysaccharide and cell wall biogenesis, and in regulation of virulence play a role in calcium precipitation. These results shed light on the common ability of Pseudomonas species to perform calcium precipitation and the underlying genetic regulation involved in biomineralization.
format	article
author	Maxwell R. Fishman Krista Giglio David Fay Melanie J. Filiatrault
author_facet	Maxwell R. Fishman Krista Giglio David Fay Melanie J. Filiatrault
author_sort	Maxwell R. Fishman
title	Physiological and genetic characterization of calcium phosphate precipitation by Pseudomonas species
title_short	Physiological and genetic characterization of calcium phosphate precipitation by Pseudomonas species
title_full	Physiological and genetic characterization of calcium phosphate precipitation by Pseudomonas species
title_fullStr	Physiological and genetic characterization of calcium phosphate precipitation by Pseudomonas species
title_full_unstemmed	Physiological and genetic characterization of calcium phosphate precipitation by Pseudomonas species
title_sort	physiological and genetic characterization of calcium phosphate precipitation by pseudomonas species
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/bc1ccfaa8f6f45e0a1d0e83df7490092
work_keys_str_mv	AT maxwellrfishman physiologicalandgeneticcharacterizationofcalciumphosphateprecipitationbypseudomonasspecies AT kristagiglio physiologicalandgeneticcharacterizationofcalciumphosphateprecipitationbypseudomonasspecies AT davidfay physiologicalandgeneticcharacterizationofcalciumphosphateprecipitationbypseudomonasspecies AT melaniejfiliatrault physiologicalandgeneticcharacterizationofcalciumphosphateprecipitationbypseudomonasspecies
_version_	1718388209157668864

Physiological and genetic characterization of calcium phosphate precipitation by Pseudomonas species

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