Protective effects of Resveratrol on inflammation and oxidative stress damage in human corneal epithelial cells

AIM:To investigate the protective effects of resveratrol(RSV)on inflammation and oxidative stress damage in human corneal epithelial cells(HCECs).METHODS: The inflammation of HCECs was induced by Tumor necrosis factor-α(TNF-α), and the experiment was divided into: control group, TNF-α group and RSV+...

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Autores principales:	Lin Zhu, Rui-Fang Han, Pei-Hong Wang, Xuan Li
Formato:	article
Lenguaje:	EN
Publicado:	Press of International Journal of Ophthalmology (IJO PRESS) 2021
Materias:	resveratrol corneal epithelial cells inflammatory response oxidative stress response nf-κb Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/bc1d5327d35648019faf7aa2f98c406d
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spelling	oai:doaj.org-article:bc1d5327d35648019faf7aa2f98c406d2021-11-22T14:22:32ZProtective effects of Resveratrol on inflammation and oxidative stress damage in human corneal epithelial cells1672-512310.3980/j.issn.1672-5123.2021.12.04https://doaj.org/article/bc1d5327d35648019faf7aa2f98c406d2021-12-01T00:00:00Zhttp://ies.ijo.cn/cn_publish/2021/12/202112004.pdfhttps://doaj.org/toc/1672-5123AIM:To investigate the protective effects of resveratrol(RSV)on inflammation and oxidative stress damage in human corneal epithelial cells(HCECs).METHODS: The inflammation of HCECs was induced by Tumor necrosis factor-α(TNF-α), and the experiment was divided into: control group, TNF-α group and RSV+TNF-α group. The oxidative stress response of HCECs was induced by H2O2, and they were divided into normal group, H2O2 group and RSV+ H2O2 group. MTT assay was used to detect the viability of HCECs; RT-qPCR and enzyme-linked immunosorbent assay(ELISA)methods were used to detect the expression of IL-1, IL-6 and IL-8; Immunofluorescence staining and Western blot were used to observe the nuclear translocation of NF-κB p65. 2',7'-dichlorofluorescein diacetate(DCFH-DA)fluorescent probe was applied to detect the level of reactive oxygen species(ROS).RESULTS:In the inflammatory response of HCECs, RT-qPCR and ELISA showed that the expression levels of IL-1, IL-6 and IL-8 were increased significantly in the TNF-α group compared with the control group, the above indicators were lower after pretreatment of RSV than those in TNF-α group; Immunofluorescence staining and Western blot showed that the nuclear translocation of NF-κB p65 was increased in TNF-α group, while it was inhibited after pretreatment of RSV. In the oxidative stress response of HCECs, the results of MTT and DCFH-DA fluorescent probe staining showed that H2O2 significantly decreased the viability of HCECs and increased the production of ROS in HCECs. After pretreatment of RSV, cell viability increased significantly, and RSV inhibited the generation of ROS in HCECs induced by H2O2. CONCLUSION: RSV has an inhibitory effect on inflammation and oxidative stress damage in human corneal epithelial cells, and it has been confirmed that RSV inhibits inflammation by inhibiting the activation of the NF-κB pathway.Lin ZhuRui-Fang HanPei-Hong WangXuan LiPress of International Journal of Ophthalmology (IJO PRESS)articleresveratrolcorneal epithelial cellsinflammatory responseoxidative stress responsenf-κbOphthalmologyRE1-994ENGuoji Yanke Zazhi, Vol 21, Iss 12, Pp 2038-2043 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	resveratrol corneal epithelial cells inflammatory response oxidative stress response nf-κb Ophthalmology RE1-994
spellingShingle	resveratrol corneal epithelial cells inflammatory response oxidative stress response nf-κb Ophthalmology RE1-994 Lin Zhu Rui-Fang Han Pei-Hong Wang Xuan Li Protective effects of Resveratrol on inflammation and oxidative stress damage in human corneal epithelial cells
description	AIM:To investigate the protective effects of resveratrol(RSV)on inflammation and oxidative stress damage in human corneal epithelial cells(HCECs).METHODS: The inflammation of HCECs was induced by Tumor necrosis factor-α(TNF-α), and the experiment was divided into: control group, TNF-α group and RSV+TNF-α group. The oxidative stress response of HCECs was induced by H2O2, and they were divided into normal group, H2O2 group and RSV+ H2O2 group. MTT assay was used to detect the viability of HCECs; RT-qPCR and enzyme-linked immunosorbent assay(ELISA)methods were used to detect the expression of IL-1, IL-6 and IL-8; Immunofluorescence staining and Western blot were used to observe the nuclear translocation of NF-κB p65. 2',7'-dichlorofluorescein diacetate(DCFH-DA)fluorescent probe was applied to detect the level of reactive oxygen species(ROS).RESULTS:In the inflammatory response of HCECs, RT-qPCR and ELISA showed that the expression levels of IL-1, IL-6 and IL-8 were increased significantly in the TNF-α group compared with the control group, the above indicators were lower after pretreatment of RSV than those in TNF-α group; Immunofluorescence staining and Western blot showed that the nuclear translocation of NF-κB p65 was increased in TNF-α group, while it was inhibited after pretreatment of RSV. In the oxidative stress response of HCECs, the results of MTT and DCFH-DA fluorescent probe staining showed that H2O2 significantly decreased the viability of HCECs and increased the production of ROS in HCECs. After pretreatment of RSV, cell viability increased significantly, and RSV inhibited the generation of ROS in HCECs induced by H2O2. CONCLUSION: RSV has an inhibitory effect on inflammation and oxidative stress damage in human corneal epithelial cells, and it has been confirmed that RSV inhibits inflammation by inhibiting the activation of the NF-κB pathway.
format	article
author	Lin Zhu Rui-Fang Han Pei-Hong Wang Xuan Li
author_facet	Lin Zhu Rui-Fang Han Pei-Hong Wang Xuan Li
author_sort	Lin Zhu
title	Protective effects of Resveratrol on inflammation and oxidative stress damage in human corneal epithelial cells
title_short	Protective effects of Resveratrol on inflammation and oxidative stress damage in human corneal epithelial cells
title_full	Protective effects of Resveratrol on inflammation and oxidative stress damage in human corneal epithelial cells
title_fullStr	Protective effects of Resveratrol on inflammation and oxidative stress damage in human corneal epithelial cells
title_full_unstemmed	Protective effects of Resveratrol on inflammation and oxidative stress damage in human corneal epithelial cells
title_sort	protective effects of resveratrol on inflammation and oxidative stress damage in human corneal epithelial cells
publisher	Press of International Journal of Ophthalmology (IJO PRESS)
publishDate	2021
url	https://doaj.org/article/bc1d5327d35648019faf7aa2f98c406d
work_keys_str_mv	AT linzhu protectiveeffectsofresveratroloninflammationandoxidativestressdamageinhumancornealepithelialcells AT ruifanghan protectiveeffectsofresveratroloninflammationandoxidativestressdamageinhumancornealepithelialcells AT peihongwang protectiveeffectsofresveratroloninflammationandoxidativestressdamageinhumancornealepithelialcells AT xuanli protectiveeffectsofresveratroloninflammationandoxidativestressdamageinhumancornealepithelialcells
_version_	1718417576728461312

Protective effects of Resveratrol on inflammation and oxidative stress damage in human corneal epithelial cells

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