Evaluating very high energy electron RBE from nanodosimetric pBR322 plasmid DNA damage

Abstract This paper presents the first plasmid DNA irradiations carried out with Very High Energy Electrons (VHEE) over 100–200 MeV at the CLEAR user facility at CERN to determine the Relative Biological Effectiveness (RBE) of VHEE. DNA damage yields were measured in dry and aqueous environments to...

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Autores principales:	K. L. Small, N. T. Henthorn, D. Angal-Kalinin, A. L. Chadwick, E. Santina, A. Aitkenhead, K. J. Kirkby, R. J. Smith, M. Surman, J. Jones, W. Farabolini, R. Corsini, D. Gamba, A. Gilardi, M. J. Merchant, R. M. Jones
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc24ff85f48f4675af445482c6dc0f31
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spelling	oai:doaj.org-article:bc24ff85f48f4675af445482c6dc0f312021-12-02T14:11:31ZEvaluating very high energy electron RBE from nanodosimetric pBR322 plasmid DNA damage10.1038/s41598-021-82772-62045-2322https://doaj.org/article/bc24ff85f48f4675af445482c6dc0f312021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82772-6https://doaj.org/toc/2045-2322Abstract This paper presents the first plasmid DNA irradiations carried out with Very High Energy Electrons (VHEE) over 100–200 MeV at the CLEAR user facility at CERN to determine the Relative Biological Effectiveness (RBE) of VHEE. DNA damage yields were measured in dry and aqueous environments to determine that ~ 99% of total DNA breaks were caused by indirect effects, consistent with other published measurements for protons and photons. Double-Strand Break (DSB) yield was used as the biological endpoint for RBE calculation, with values found to be consistent with established radiotherapy modalities. Similarities in physical damage between VHEE and conventional modalities gives confidence that biological effects of VHEE will also be similar—key for clinical implementation. Damage yields were used as a baseline for track structure simulations of VHEE plasmid irradiation using GEANT4-DNA. Current models for DSB yield have shown reasonable agreement with experimental values. The growing interest in FLASH radiotherapy motivated a study into DSB yield variation with dose rate following VHEE irradiation. No significant variations were observed between conventional and FLASH dose rate irradiations, indicating that no FLASH effect is seen under these conditions.K. L. SmallN. T. HenthornD. Angal-KalininA. L. ChadwickE. SantinaA. AitkenheadK. J. KirkbyR. J. SmithM. SurmanJ. JonesW. FaraboliniR. CorsiniD. GambaA. GilardiM. J. MerchantR. M. JonesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q K. L. Small N. T. Henthorn D. Angal-Kalinin A. L. Chadwick E. Santina A. Aitkenhead K. J. Kirkby R. J. Smith M. Surman J. Jones W. Farabolini R. Corsini D. Gamba A. Gilardi M. J. Merchant R. M. Jones Evaluating very high energy electron RBE from nanodosimetric pBR322 plasmid DNA damage
description	Abstract This paper presents the first plasmid DNA irradiations carried out with Very High Energy Electrons (VHEE) over 100–200 MeV at the CLEAR user facility at CERN to determine the Relative Biological Effectiveness (RBE) of VHEE. DNA damage yields were measured in dry and aqueous environments to determine that ~ 99% of total DNA breaks were caused by indirect effects, consistent with other published measurements for protons and photons. Double-Strand Break (DSB) yield was used as the biological endpoint for RBE calculation, with values found to be consistent with established radiotherapy modalities. Similarities in physical damage between VHEE and conventional modalities gives confidence that biological effects of VHEE will also be similar—key for clinical implementation. Damage yields were used as a baseline for track structure simulations of VHEE plasmid irradiation using GEANT4-DNA. Current models for DSB yield have shown reasonable agreement with experimental values. The growing interest in FLASH radiotherapy motivated a study into DSB yield variation with dose rate following VHEE irradiation. No significant variations were observed between conventional and FLASH dose rate irradiations, indicating that no FLASH effect is seen under these conditions.
format	article
author	K. L. Small N. T. Henthorn D. Angal-Kalinin A. L. Chadwick E. Santina A. Aitkenhead K. J. Kirkby R. J. Smith M. Surman J. Jones W. Farabolini R. Corsini D. Gamba A. Gilardi M. J. Merchant R. M. Jones
author_facet	K. L. Small N. T. Henthorn D. Angal-Kalinin A. L. Chadwick E. Santina A. Aitkenhead K. J. Kirkby R. J. Smith M. Surman J. Jones W. Farabolini R. Corsini D. Gamba A. Gilardi M. J. Merchant R. M. Jones
author_sort	K. L. Small
title	Evaluating very high energy electron RBE from nanodosimetric pBR322 plasmid DNA damage
title_short	Evaluating very high energy electron RBE from nanodosimetric pBR322 plasmid DNA damage
title_full	Evaluating very high energy electron RBE from nanodosimetric pBR322 plasmid DNA damage
title_fullStr	Evaluating very high energy electron RBE from nanodosimetric pBR322 plasmid DNA damage
title_full_unstemmed	Evaluating very high energy electron RBE from nanodosimetric pBR322 plasmid DNA damage
title_sort	evaluating very high energy electron rbe from nanodosimetric pbr322 plasmid dna damage
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bc24ff85f48f4675af445482c6dc0f31
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Evaluating very high energy electron RBE from nanodosimetric pBR322 plasmid DNA damage

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