Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains

Abstract Cryopreservation of mouse spermatozoa is widely used for the efficient preservation and safe transport of valuable mouse strains. However, the current cryopreservation method requires special containers (plastic straws), undefined chemicals (e.g., skim milk), liquid nitrogen, and expertise...

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Autores principales:	Keiji Mochida, Ayumi Hasegawa, Daiki Shikata, Nobuhiko Itami, Masashi Hada, Naomi Watanabe, Toshiko Tomishima, Atsuo Ogura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc39811397874fd4bcdd714e9d6a1b3d
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spelling	oai:doaj.org-article:bc39811397874fd4bcdd714e9d6a1b3d2021-12-02T15:40:00ZEasy and quick (EQ) sperm freezing method for urgent preservation of mouse strains10.1038/s41598-021-93604-y2045-2322https://doaj.org/article/bc39811397874fd4bcdd714e9d6a1b3d2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93604-yhttps://doaj.org/toc/2045-2322Abstract Cryopreservation of mouse spermatozoa is widely used for the efficient preservation and safe transport of valuable mouse strains. However, the current cryopreservation method requires special containers (plastic straws), undefined chemicals (e.g., skim milk), liquid nitrogen, and expertise when handling sperm suspensions. Here, we report an easy and quick (EQ) sperm freezing method. The main procedure consists of only one step: dissecting a single cauda epididymis in a microtube containing 20% raffinose solution, which is then stored in a −80 °C freezer. The frozen–thawed spermatozoa retain practical fertilization rates after 1 (51%) or even 3 months (25%) with the C57BL/6 J strain, the most sensitive strain for sperm freezing. More than half of the embryos thus obtained developed into offspring after embryo transfer. Importantly, spermatozoa stored at −80 °C can be transferred into liquid nitrogen for indefinite storage. As far as we know, our EQ method is the easiest and quickest method for mouse sperm freezing and should be applicable in all laboratories without expertise in sperm cryopreservation. This technique can help avoid the loss of irreplaceable strains because of closure of animal rooms in emergency situations such as unexpected microbiological contamination or social emergencies such as the COVID-19 threat.Keiji MochidaAyumi HasegawaDaiki ShikataNobuhiko ItamiMasashi HadaNaomi WatanabeToshiko TomishimaAtsuo OguraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keiji Mochida Ayumi Hasegawa Daiki Shikata Nobuhiko Itami Masashi Hada Naomi Watanabe Toshiko Tomishima Atsuo Ogura Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains
description	Abstract Cryopreservation of mouse spermatozoa is widely used for the efficient preservation and safe transport of valuable mouse strains. However, the current cryopreservation method requires special containers (plastic straws), undefined chemicals (e.g., skim milk), liquid nitrogen, and expertise when handling sperm suspensions. Here, we report an easy and quick (EQ) sperm freezing method. The main procedure consists of only one step: dissecting a single cauda epididymis in a microtube containing 20% raffinose solution, which is then stored in a −80 °C freezer. The frozen–thawed spermatozoa retain practical fertilization rates after 1 (51%) or even 3 months (25%) with the C57BL/6 J strain, the most sensitive strain for sperm freezing. More than half of the embryos thus obtained developed into offspring after embryo transfer. Importantly, spermatozoa stored at −80 °C can be transferred into liquid nitrogen for indefinite storage. As far as we know, our EQ method is the easiest and quickest method for mouse sperm freezing and should be applicable in all laboratories without expertise in sperm cryopreservation. This technique can help avoid the loss of irreplaceable strains because of closure of animal rooms in emergency situations such as unexpected microbiological contamination or social emergencies such as the COVID-19 threat.
format	article
author	Keiji Mochida Ayumi Hasegawa Daiki Shikata Nobuhiko Itami Masashi Hada Naomi Watanabe Toshiko Tomishima Atsuo Ogura
author_facet	Keiji Mochida Ayumi Hasegawa Daiki Shikata Nobuhiko Itami Masashi Hada Naomi Watanabe Toshiko Tomishima Atsuo Ogura
author_sort	Keiji Mochida
title	Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains
title_short	Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains
title_full	Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains
title_fullStr	Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains
title_full_unstemmed	Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains
title_sort	easy and quick (eq) sperm freezing method for urgent preservation of mouse strains
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bc39811397874fd4bcdd714e9d6a1b3d
work_keys_str_mv	AT keijimochida easyandquickeqspermfreezingmethodforurgentpreservationofmousestrains AT ayumihasegawa easyandquickeqspermfreezingmethodforurgentpreservationofmousestrains AT daikishikata easyandquickeqspermfreezingmethodforurgentpreservationofmousestrains AT nobuhikoitami easyandquickeqspermfreezingmethodforurgentpreservationofmousestrains AT masashihada easyandquickeqspermfreezingmethodforurgentpreservationofmousestrains AT naomiwatanabe easyandquickeqspermfreezingmethodforurgentpreservationofmousestrains AT toshikotomishima easyandquickeqspermfreezingmethodforurgentpreservationofmousestrains AT atsuoogura easyandquickeqspermfreezingmethodforurgentpreservationofmousestrains
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Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains

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