Chromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program

ABSTRACT Recent studies strongly suggest that the gene expression sustaining both normal and pathogenic bacterial growth is governed by the structural dynamics of the chromosome. However, the mechanistic device coordinating the chromosomal configuration with selective expression of the adaptive trai...

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Autores principales: Xuejiao Jiang, Patrick Sobetzko, William Nasser, Sylvie Reverchon, Georgi Muskhelishvili
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Publicado: American Society for Microbiology 2015
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spelling oai:doaj.org-article:bc3a5d8c65ba41c4985af17c736d10bf2021-11-15T15:49:02ZChromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program10.1128/mBio.00353-152150-7511https://doaj.org/article/bc3a5d8c65ba41c4985af17c736d10bf2015-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00353-15https://doaj.org/toc/2150-7511ABSTRACT Recent studies strongly suggest that the gene expression sustaining both normal and pathogenic bacterial growth is governed by the structural dynamics of the chromosome. However, the mechanistic device coordinating the chromosomal configuration with selective expression of the adaptive traits remains largely unknown. We used a holistic approach exploring the inherent relationships between the physicochemical properties of the DNA and the expression of adaptive traits, including virulence factors, in the pathogen Dickeya dadantii (formerly Erwinia chrysanthemi). In the transcriptomes obtained under adverse conditions encountered during bacterial infection, we explored the patterns of chromosomal DNA sequence organization, supercoil dynamics, and gene expression densities, together with the long-range regulatory impacts of the abundant DNA architectural proteins implicated in pathogenicity control. By integrating these data, we identified transient chromosomal domains of coherent gene expression featuring distinct couplings between DNA thermodynamic stability, supercoil dynamics, and virulence traits. IMPORTANCE We infer that the organization of transient chromosomal domains serving specific functions acts as a fundamental device for versatile adjustment of the pathogen to environmental stress. We believe that the identification of chromosomal “stress-response” domains harboring distinct virulence traits and mediating the cellular adaptive behavior provides a breakthrough in understanding the control mechanisms of bacterial pathogenicity.Xuejiao JiangPatrick SobetzkoWilliam NasserSylvie ReverchonGeorgi MuskhelishviliAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 6, Iss 3 (2015)
institution DOAJ
collection DOAJ
language EN
topic Microbiology
QR1-502
spellingShingle Microbiology
QR1-502
Xuejiao Jiang
Patrick Sobetzko
William Nasser
Sylvie Reverchon
Georgi Muskhelishvili
Chromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program
description ABSTRACT Recent studies strongly suggest that the gene expression sustaining both normal and pathogenic bacterial growth is governed by the structural dynamics of the chromosome. However, the mechanistic device coordinating the chromosomal configuration with selective expression of the adaptive traits remains largely unknown. We used a holistic approach exploring the inherent relationships between the physicochemical properties of the DNA and the expression of adaptive traits, including virulence factors, in the pathogen Dickeya dadantii (formerly Erwinia chrysanthemi). In the transcriptomes obtained under adverse conditions encountered during bacterial infection, we explored the patterns of chromosomal DNA sequence organization, supercoil dynamics, and gene expression densities, together with the long-range regulatory impacts of the abundant DNA architectural proteins implicated in pathogenicity control. By integrating these data, we identified transient chromosomal domains of coherent gene expression featuring distinct couplings between DNA thermodynamic stability, supercoil dynamics, and virulence traits. IMPORTANCE We infer that the organization of transient chromosomal domains serving specific functions acts as a fundamental device for versatile adjustment of the pathogen to environmental stress. We believe that the identification of chromosomal “stress-response” domains harboring distinct virulence traits and mediating the cellular adaptive behavior provides a breakthrough in understanding the control mechanisms of bacterial pathogenicity.
format article
author Xuejiao Jiang
Patrick Sobetzko
William Nasser
Sylvie Reverchon
Georgi Muskhelishvili
author_facet Xuejiao Jiang
Patrick Sobetzko
William Nasser
Sylvie Reverchon
Georgi Muskhelishvili
author_sort Xuejiao Jiang
title Chromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program
title_short Chromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program
title_full Chromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program
title_fullStr Chromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program
title_full_unstemmed Chromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program
title_sort chromosomal “stress-response” domains govern the spatiotemporal expression of the bacterial virulence program
publisher American Society for Microbiology
publishDate 2015
url https://doaj.org/article/bc3a5d8c65ba41c4985af17c736d10bf
work_keys_str_mv AT xuejiaojiang chromosomalstressresponsedomainsgovernthespatiotemporalexpressionofthebacterialvirulenceprogram
AT patricksobetzko chromosomalstressresponsedomainsgovernthespatiotemporalexpressionofthebacterialvirulenceprogram
AT williamnasser chromosomalstressresponsedomainsgovernthespatiotemporalexpressionofthebacterialvirulenceprogram
AT sylviereverchon chromosomalstressresponsedomainsgovernthespatiotemporalexpressionofthebacterialvirulenceprogram
AT georgimuskhelishvili chromosomalstressresponsedomainsgovernthespatiotemporalexpressionofthebacterialvirulenceprogram
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