Chromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program
ABSTRACT Recent studies strongly suggest that the gene expression sustaining both normal and pathogenic bacterial growth is governed by the structural dynamics of the chromosome. However, the mechanistic device coordinating the chromosomal configuration with selective expression of the adaptive trai...
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American Society for Microbiology
2015
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oai:doaj.org-article:bc3a5d8c65ba41c4985af17c736d10bf2021-11-15T15:49:02ZChromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program10.1128/mBio.00353-152150-7511https://doaj.org/article/bc3a5d8c65ba41c4985af17c736d10bf2015-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00353-15https://doaj.org/toc/2150-7511ABSTRACT Recent studies strongly suggest that the gene expression sustaining both normal and pathogenic bacterial growth is governed by the structural dynamics of the chromosome. However, the mechanistic device coordinating the chromosomal configuration with selective expression of the adaptive traits remains largely unknown. We used a holistic approach exploring the inherent relationships between the physicochemical properties of the DNA and the expression of adaptive traits, including virulence factors, in the pathogen Dickeya dadantii (formerly Erwinia chrysanthemi). In the transcriptomes obtained under adverse conditions encountered during bacterial infection, we explored the patterns of chromosomal DNA sequence organization, supercoil dynamics, and gene expression densities, together with the long-range regulatory impacts of the abundant DNA architectural proteins implicated in pathogenicity control. By integrating these data, we identified transient chromosomal domains of coherent gene expression featuring distinct couplings between DNA thermodynamic stability, supercoil dynamics, and virulence traits. IMPORTANCE We infer that the organization of transient chromosomal domains serving specific functions acts as a fundamental device for versatile adjustment of the pathogen to environmental stress. We believe that the identification of chromosomal “stress-response” domains harboring distinct virulence traits and mediating the cellular adaptive behavior provides a breakthrough in understanding the control mechanisms of bacterial pathogenicity.Xuejiao JiangPatrick SobetzkoWilliam NasserSylvie ReverchonGeorgi MuskhelishviliAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 6, Iss 3 (2015) |
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Microbiology QR1-502 |
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Microbiology QR1-502 Xuejiao Jiang Patrick Sobetzko William Nasser Sylvie Reverchon Georgi Muskhelishvili Chromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program |
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ABSTRACT Recent studies strongly suggest that the gene expression sustaining both normal and pathogenic bacterial growth is governed by the structural dynamics of the chromosome. However, the mechanistic device coordinating the chromosomal configuration with selective expression of the adaptive traits remains largely unknown. We used a holistic approach exploring the inherent relationships between the physicochemical properties of the DNA and the expression of adaptive traits, including virulence factors, in the pathogen Dickeya dadantii (formerly Erwinia chrysanthemi). In the transcriptomes obtained under adverse conditions encountered during bacterial infection, we explored the patterns of chromosomal DNA sequence organization, supercoil dynamics, and gene expression densities, together with the long-range regulatory impacts of the abundant DNA architectural proteins implicated in pathogenicity control. By integrating these data, we identified transient chromosomal domains of coherent gene expression featuring distinct couplings between DNA thermodynamic stability, supercoil dynamics, and virulence traits. IMPORTANCE We infer that the organization of transient chromosomal domains serving specific functions acts as a fundamental device for versatile adjustment of the pathogen to environmental stress. We believe that the identification of chromosomal “stress-response” domains harboring distinct virulence traits and mediating the cellular adaptive behavior provides a breakthrough in understanding the control mechanisms of bacterial pathogenicity. |
format |
article |
author |
Xuejiao Jiang Patrick Sobetzko William Nasser Sylvie Reverchon Georgi Muskhelishvili |
author_facet |
Xuejiao Jiang Patrick Sobetzko William Nasser Sylvie Reverchon Georgi Muskhelishvili |
author_sort |
Xuejiao Jiang |
title |
Chromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program |
title_short |
Chromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program |
title_full |
Chromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program |
title_fullStr |
Chromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program |
title_full_unstemmed |
Chromosomal “Stress-Response” Domains Govern the Spatiotemporal Expression of the Bacterial Virulence Program |
title_sort |
chromosomal “stress-response” domains govern the spatiotemporal expression of the bacterial virulence program |
publisher |
American Society for Microbiology |
publishDate |
2015 |
url |
https://doaj.org/article/bc3a5d8c65ba41c4985af17c736d10bf |
work_keys_str_mv |
AT xuejiaojiang chromosomalstressresponsedomainsgovernthespatiotemporalexpressionofthebacterialvirulenceprogram AT patricksobetzko chromosomalstressresponsedomainsgovernthespatiotemporalexpressionofthebacterialvirulenceprogram AT williamnasser chromosomalstressresponsedomainsgovernthespatiotemporalexpressionofthebacterialvirulenceprogram AT sylviereverchon chromosomalstressresponsedomainsgovernthespatiotemporalexpressionofthebacterialvirulenceprogram AT georgimuskhelishvili chromosomalstressresponsedomainsgovernthespatiotemporalexpressionofthebacterialvirulenceprogram |
_version_ |
1718427544055709696 |