Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers

Abstract. Panaligan AC, Baltazar MD, Alejandro GJD. 2020. Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers. Biodiversitas 21: 4228-4233. Three Coffea species, namely Coffea arabica, Co...

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Autores principales:	Arleen Panaligan, MIRIAM D. BALTAZAR, GRECEBIO JONATHAN D. ALEJANDRO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	coffea, coffee, genetic diversity, inter-simple sequence repeat (issr), polymorphism Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bc3d34a76c9446febb61fab71d6eb175
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spelling	oai:doaj.org-article:bc3d34a76c9446febb61fab71d6eb1752021-11-22T00:41:43ZShort Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers1412-033X2085-472210.13057/biodiv/d210938https://doaj.org/article/bc3d34a76c9446febb61fab71d6eb1752020-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6165https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Panaligan AC, Baltazar MD, Alejandro GJD. 2020. Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers. Biodiversitas 21: 4228-4233. Three Coffea species, namely Coffea arabica, Coffea canephora and Coffea liberica have been commercially cultivated in the Philippines. Genetic variability analysis of these species is important for the conservation of genetic resources and breeding programs. Hence, this study was carried out to identify polymorphic inter-simple sequence repeats (ISSR) markers and determine the genetic variation of these three commercially cultivated coffee species. Twenty-nine DNA samples from young coffee leaves were extracted and PCR amplified. Of the 29 primers used, seven produced clear and reproducible bands. In the 54 bands produced, 51 were polymorphic. The number of bands amplified by each primer varied from 5 to 12 with an average of 7.71 bands. Polymorphism percentage ranged from 80 to 100. This is the first time that ISSR markers were used to determine the genetic variation of coffee in the Philippines. The study demonstrated the efficiency of ISSR markers to assess genetic variation in cultivated coffee species. The ISSR markers were able to differentiate the coffee germplasm at the interspecific and intraspecific levels. These results suggest the potential of ISSR markers for genetic diversity analysis of commercial coffee and varietal identification of elite varieties using DNA fingerprinting.Arleen PanaliganMIRIAM D. BALTAZARGRECEBIO JONATHAN D. ALEJANDROMBI & UNS Soloarticlecoffea, coffee, genetic diversity, inter-simple sequence repeat (issr), polymorphismBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	coffea, coffee, genetic diversity, inter-simple sequence repeat (issr), polymorphism Biology (General) QH301-705.5
spellingShingle	coffea, coffee, genetic diversity, inter-simple sequence repeat (issr), polymorphism Biology (General) QH301-705.5 Arleen Panaligan MIRIAM D. BALTAZAR GRECEBIO JONATHAN D. ALEJANDRO Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers
description	Abstract. Panaligan AC, Baltazar MD, Alejandro GJD. 2020. Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers. Biodiversitas 21: 4228-4233. Three Coffea species, namely Coffea arabica, Coffea canephora and Coffea liberica have been commercially cultivated in the Philippines. Genetic variability analysis of these species is important for the conservation of genetic resources and breeding programs. Hence, this study was carried out to identify polymorphic inter-simple sequence repeats (ISSR) markers and determine the genetic variation of these three commercially cultivated coffee species. Twenty-nine DNA samples from young coffee leaves were extracted and PCR amplified. Of the 29 primers used, seven produced clear and reproducible bands. In the 54 bands produced, 51 were polymorphic. The number of bands amplified by each primer varied from 5 to 12 with an average of 7.71 bands. Polymorphism percentage ranged from 80 to 100. This is the first time that ISSR markers were used to determine the genetic variation of coffee in the Philippines. The study demonstrated the efficiency of ISSR markers to assess genetic variation in cultivated coffee species. The ISSR markers were able to differentiate the coffee germplasm at the interspecific and intraspecific levels. These results suggest the potential of ISSR markers for genetic diversity analysis of commercial coffee and varietal identification of elite varieties using DNA fingerprinting.
format	article
author	Arleen Panaligan MIRIAM D. BALTAZAR GRECEBIO JONATHAN D. ALEJANDRO
author_facet	Arleen Panaligan MIRIAM D. BALTAZAR GRECEBIO JONATHAN D. ALEJANDRO
author_sort	Arleen Panaligan
title	Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers
title_short	Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers
title_full	Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers
title_fullStr	Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers
title_full_unstemmed	Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers
title_sort	short communication: genetic polymorphism of registered and popularly cultivated coffee (coffea spp.) in the philippines using inter-simple sequence repeats markers
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/bc3d34a76c9446febb61fab71d6eb175
work_keys_str_mv	AT arleenpanaligan shortcommunicationgeneticpolymorphismofregisteredandpopularlycultivatedcoffeecoffeasppinthephilippinesusingintersimplesequencerepeatsmarkers AT miriamdbaltazar shortcommunicationgeneticpolymorphismofregisteredandpopularlycultivatedcoffeecoffeasppinthephilippinesusingintersimplesequencerepeatsmarkers AT grecebiojonathandalejandro shortcommunicationgeneticpolymorphismofregisteredandpopularlycultivatedcoffeecoffeasppinthephilippinesusingintersimplesequencerepeatsmarkers
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Short Communication: Genetic polymorphism of registered and popularly cultivated coffee (Coffea spp.) in the Philippines using inter-simple sequence repeats markers

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