MALDI-TOF mass spectrometry for the identification of freshwater snails from Senegal, including intermediate hosts of schistosomes.

Freshwater snails of the genera Biomphalaria, Bulinus, and Oncomelania are intermediate hosts of schistosomes that cause human schistosomiasis, one of the most significant infectious neglected diseases in the world. Identification of freshwater snails is usually based on morphology and potentially D...

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Autores principales:	Fatima Zohra Hamlili, Fatou Thiam, Maureen Laroche, Adama Zan Diarra, Souleymane Doucouré, Papa Mouhamadou Gaye, Cheikh Binetou Fall, Babacar Faye, Cheikh Sokhna, Doudou Sow, Philippe Parola
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/bc4c60b359344d5ea90f8406affbadb0
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spelling	oai:doaj.org-article:bc4c60b359344d5ea90f8406affbadb02021-12-02T20:24:07ZMALDI-TOF mass spectrometry for the identification of freshwater snails from Senegal, including intermediate hosts of schistosomes.1935-27271935-273510.1371/journal.pntd.0009725https://doaj.org/article/bc4c60b359344d5ea90f8406affbadb02021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009725https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Freshwater snails of the genera Biomphalaria, Bulinus, and Oncomelania are intermediate hosts of schistosomes that cause human schistosomiasis, one of the most significant infectious neglected diseases in the world. Identification of freshwater snails is usually based on morphology and potentially DNA-based methods, but these have many drawbacks that hamper their use. MALDI-TOF MS has revolutionised clinical microbiology and has emerged in the medical entomology field. This study aims to evaluate MALDI-TOF MS profiling for the identification of both frozen and ethanol-stored snail species using protein extracts from different body parts. A total of 530 field specimens belonging to nine species (Biomphalaria pfeifferi, Bulinus forskalii, Bulinus senegalensis, Bulinus truncatus, Bulinus globosus, Bellamya unicolor, Cleopatra bulimoides, Lymnaea natalensis, Melanoides tuberculata) and 89 laboratory-reared specimens, including three species (Bi. pfeifferi, Bu. forskalii, Bu. truncatus) were used for this study. For frozen snails, the feet of 127 field and 74 laboratory-reared specimens were used to validate the optimised MALDI-TOF MS protocol. The spectral analysis yielded intra-species reproducibility and inter-species specificity which resulted in the correct identification of all the specimens in blind queries, with log-score values greater than 1.7. In a second step, we demonstrated that MALDI-TOF MS could also be used to identify ethanol-stored snails using proteins extracted from the foot using a specific database including a large number of ethanol preserved specimens. This study shows for the first time that MALDI-TOF MS is a reliable tool for the rapid identification of frozen and ethanol-stored freshwater snails without any malacological expertise.Fatima Zohra HamliliFatou ThiamMaureen LarocheAdama Zan DiarraSouleymane DoucouréPapa Mouhamadou GayeCheikh Binetou FallBabacar FayeCheikh SokhnaDoudou SowPhilippe ParolaPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 9, p e0009725 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Fatima Zohra Hamlili Fatou Thiam Maureen Laroche Adama Zan Diarra Souleymane Doucouré Papa Mouhamadou Gaye Cheikh Binetou Fall Babacar Faye Cheikh Sokhna Doudou Sow Philippe Parola MALDI-TOF mass spectrometry for the identification of freshwater snails from Senegal, including intermediate hosts of schistosomes.
description	Freshwater snails of the genera Biomphalaria, Bulinus, and Oncomelania are intermediate hosts of schistosomes that cause human schistosomiasis, one of the most significant infectious neglected diseases in the world. Identification of freshwater snails is usually based on morphology and potentially DNA-based methods, but these have many drawbacks that hamper their use. MALDI-TOF MS has revolutionised clinical microbiology and has emerged in the medical entomology field. This study aims to evaluate MALDI-TOF MS profiling for the identification of both frozen and ethanol-stored snail species using protein extracts from different body parts. A total of 530 field specimens belonging to nine species (Biomphalaria pfeifferi, Bulinus forskalii, Bulinus senegalensis, Bulinus truncatus, Bulinus globosus, Bellamya unicolor, Cleopatra bulimoides, Lymnaea natalensis, Melanoides tuberculata) and 89 laboratory-reared specimens, including three species (Bi. pfeifferi, Bu. forskalii, Bu. truncatus) were used for this study. For frozen snails, the feet of 127 field and 74 laboratory-reared specimens were used to validate the optimised MALDI-TOF MS protocol. The spectral analysis yielded intra-species reproducibility and inter-species specificity which resulted in the correct identification of all the specimens in blind queries, with log-score values greater than 1.7. In a second step, we demonstrated that MALDI-TOF MS could also be used to identify ethanol-stored snails using proteins extracted from the foot using a specific database including a large number of ethanol preserved specimens. This study shows for the first time that MALDI-TOF MS is a reliable tool for the rapid identification of frozen and ethanol-stored freshwater snails without any malacological expertise.
format	article
author	Fatima Zohra Hamlili Fatou Thiam Maureen Laroche Adama Zan Diarra Souleymane Doucouré Papa Mouhamadou Gaye Cheikh Binetou Fall Babacar Faye Cheikh Sokhna Doudou Sow Philippe Parola
author_facet	Fatima Zohra Hamlili Fatou Thiam Maureen Laroche Adama Zan Diarra Souleymane Doucouré Papa Mouhamadou Gaye Cheikh Binetou Fall Babacar Faye Cheikh Sokhna Doudou Sow Philippe Parola
author_sort	Fatima Zohra Hamlili
title	MALDI-TOF mass spectrometry for the identification of freshwater snails from Senegal, including intermediate hosts of schistosomes.
title_short	MALDI-TOF mass spectrometry for the identification of freshwater snails from Senegal, including intermediate hosts of schistosomes.
title_full	MALDI-TOF mass spectrometry for the identification of freshwater snails from Senegal, including intermediate hosts of schistosomes.
title_fullStr	MALDI-TOF mass spectrometry for the identification of freshwater snails from Senegal, including intermediate hosts of schistosomes.
title_full_unstemmed	MALDI-TOF mass spectrometry for the identification of freshwater snails from Senegal, including intermediate hosts of schistosomes.
title_sort	maldi-tof mass spectrometry for the identification of freshwater snails from senegal, including intermediate hosts of schistosomes.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/bc4c60b359344d5ea90f8406affbadb0
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MALDI-TOF mass spectrometry for the identification of freshwater snails from Senegal, including intermediate hosts of schistosomes.

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