NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model

Abstract Mice have served as an excellent model to understand the etiology of lung cancer for years. However, data regarding dual-stage carcinogenesis of lung squamous cell carcinoma (SCC) remain elusive. Therefore, we aim to develop pre-malignant (PM) and malignant (M) lung SCC in vivo using N-nitr...

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Autores principales:	Muhammad Asyaari Zakaria, Nor Fadilah Rajab, Eng Wee Chua, Gayathri Thevi Selvarajah, Siti Fathiah Masre
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc4ef5f8feac408bb5d0cd864a0ebe89
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spelling	oai:doaj.org-article:bc4ef5f8feac408bb5d0cd864a0ebe892021-11-21T12:25:27ZNTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model10.1038/s41598-021-01988-82045-2322https://doaj.org/article/bc4ef5f8feac408bb5d0cd864a0ebe892021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01988-8https://doaj.org/toc/2045-2322Abstract Mice have served as an excellent model to understand the etiology of lung cancer for years. However, data regarding dual-stage carcinogenesis of lung squamous cell carcinoma (SCC) remain elusive. Therefore, we aim to develop pre-malignant (PM) and malignant (M) lung SCC in vivo using N-nitroso-tris-chloroethylurea (NTCU). BALB/C mice were allotted into two main groups; PM and M groups which received treatment for 15 and 30 weeks, respectively. Then, the mice in each main group were allotted into three groups; control, vehicle, and cancer (n = 6), which received normal saline, 70% acetone, and 0.04 M NTCU by skin painting, respectively. Histopathologically, we discovered a mix of hyperplasia, metaplasia, and dysplasia lesions in the PM group and intracellular bridge; an SCC feature in the M group. The M group was positive for cytokeratin 5/6 protein which confirmed the lung SCC subtype. We also found significantly higher (P < 0.05) epithelium thickness in the cancer groups as compared to the vehicle and control groups at both the PM and M. Overall, this study discovered that NTCU is capable of developing PM and M lung SCC in mice model at appropriate weeks and the vehicle group was suggested to be adequate as control group for future research.Muhammad Asyaari ZakariaNor Fadilah RajabEng Wee ChuaGayathri Thevi SelvarajahSiti Fathiah MasreNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muhammad Asyaari Zakaria Nor Fadilah Rajab Eng Wee Chua Gayathri Thevi Selvarajah Siti Fathiah Masre NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
description	Abstract Mice have served as an excellent model to understand the etiology of lung cancer for years. However, data regarding dual-stage carcinogenesis of lung squamous cell carcinoma (SCC) remain elusive. Therefore, we aim to develop pre-malignant (PM) and malignant (M) lung SCC in vivo using N-nitroso-tris-chloroethylurea (NTCU). BALB/C mice were allotted into two main groups; PM and M groups which received treatment for 15 and 30 weeks, respectively. Then, the mice in each main group were allotted into three groups; control, vehicle, and cancer (n = 6), which received normal saline, 70% acetone, and 0.04 M NTCU by skin painting, respectively. Histopathologically, we discovered a mix of hyperplasia, metaplasia, and dysplasia lesions in the PM group and intracellular bridge; an SCC feature in the M group. The M group was positive for cytokeratin 5/6 protein which confirmed the lung SCC subtype. We also found significantly higher (P < 0.05) epithelium thickness in the cancer groups as compared to the vehicle and control groups at both the PM and M. Overall, this study discovered that NTCU is capable of developing PM and M lung SCC in mice model at appropriate weeks and the vehicle group was suggested to be adequate as control group for future research.
format	article
author	Muhammad Asyaari Zakaria Nor Fadilah Rajab Eng Wee Chua Gayathri Thevi Selvarajah Siti Fathiah Masre
author_facet	Muhammad Asyaari Zakaria Nor Fadilah Rajab Eng Wee Chua Gayathri Thevi Selvarajah Siti Fathiah Masre
author_sort	Muhammad Asyaari Zakaria
title	NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
title_short	NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
title_full	NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
title_fullStr	NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
title_full_unstemmed	NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
title_sort	ntcu induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bc4ef5f8feac408bb5d0cd864a0ebe89
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NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model

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