Influence of Zinc and Manganese Nanoparticles on Selected Parameters of Turkey Spermatozoa Stored in a Liquid State at 4 °C

The aim of this study was to determine the effect of semen extender supplementation with 25 or 50 μM of zinc nanoparticles (ZnNPs) or manganese nanoparticles (MnNPs) on turkey spermatozoa preserved in a liquid state. Twenty turkey ejaculates were obtained from twenty healthy males. The collected sem...

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Autores principales:	Aleksandra Orzołek, Katarzyna T. Rafalska, Wiktoria A. Otowska, Władysław Kordan, Anna J. Korzekwa, Krzysztof Kozłowski
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nanoparticles zinc manganese turkey spermatozoa Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/bc5135b85cad471e8c768a8b41d51e21
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spelling	oai:doaj.org-article:bc5135b85cad471e8c768a8b41d51e212021-11-25T16:20:35ZInfluence of Zinc and Manganese Nanoparticles on Selected Parameters of Turkey Spermatozoa Stored in a Liquid State at 4 °C10.3390/ani111132892076-2615https://doaj.org/article/bc5135b85cad471e8c768a8b41d51e212021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3289https://doaj.org/toc/2076-2615The aim of this study was to determine the effect of semen extender supplementation with 25 or 50 μM of zinc nanoparticles (ZnNPs) or manganese nanoparticles (MnNPs) on turkey spermatozoa preserved in a liquid state. Twenty turkey ejaculates were obtained from twenty healthy males. The collected semen was preserved at 4 °C for 48 h with or without NPs. Selected qualitative and quantitative parameters of sperm (motility, plasma membrane activity, mitochondrial membrane potential (MMP) and the percentage of sperm demonstrating NO and SOD activity) were examined after 2, 24 and 48 h of storage. Sperm motility and MMP decreased in semen preserved with ZnNPs at each time point of the analysis. However, all spermatozoa remained viable throughout storage. In contrast, membrane integrity and mitochondria activity (<i>p</i> ≤ 0.05) increased, and the highest SOD activity (<i>p</i> ≤ 0.05) was observed in semen preserved with MnNPs. The addition of MnNPs to the semen extender could potentially improve the parameters of turkey semen during prolonged storage.Aleksandra OrzołekKatarzyna T. RafalskaWiktoria A. OtowskaWładysław KordanAnna J. KorzekwaKrzysztof KozłowskiMDPI AGarticlenanoparticleszincmanganeseturkeyspermatozoaVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3289, p 3289 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanoparticles zinc manganese turkey spermatozoa Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	nanoparticles zinc manganese turkey spermatozoa Veterinary medicine SF600-1100 Zoology QL1-991 Aleksandra Orzołek Katarzyna T. Rafalska Wiktoria A. Otowska Władysław Kordan Anna J. Korzekwa Krzysztof Kozłowski Influence of Zinc and Manganese Nanoparticles on Selected Parameters of Turkey Spermatozoa Stored in a Liquid State at 4 °C
description	The aim of this study was to determine the effect of semen extender supplementation with 25 or 50 μM of zinc nanoparticles (ZnNPs) or manganese nanoparticles (MnNPs) on turkey spermatozoa preserved in a liquid state. Twenty turkey ejaculates were obtained from twenty healthy males. The collected semen was preserved at 4 °C for 48 h with or without NPs. Selected qualitative and quantitative parameters of sperm (motility, plasma membrane activity, mitochondrial membrane potential (MMP) and the percentage of sperm demonstrating NO and SOD activity) were examined after 2, 24 and 48 h of storage. Sperm motility and MMP decreased in semen preserved with ZnNPs at each time point of the analysis. However, all spermatozoa remained viable throughout storage. In contrast, membrane integrity and mitochondria activity (<i>p</i> ≤ 0.05) increased, and the highest SOD activity (<i>p</i> ≤ 0.05) was observed in semen preserved with MnNPs. The addition of MnNPs to the semen extender could potentially improve the parameters of turkey semen during prolonged storage.
format	article
author	Aleksandra Orzołek Katarzyna T. Rafalska Wiktoria A. Otowska Władysław Kordan Anna J. Korzekwa Krzysztof Kozłowski
author_facet	Aleksandra Orzołek Katarzyna T. Rafalska Wiktoria A. Otowska Władysław Kordan Anna J. Korzekwa Krzysztof Kozłowski
author_sort	Aleksandra Orzołek
title	Influence of Zinc and Manganese Nanoparticles on Selected Parameters of Turkey Spermatozoa Stored in a Liquid State at 4 °C
title_short	Influence of Zinc and Manganese Nanoparticles on Selected Parameters of Turkey Spermatozoa Stored in a Liquid State at 4 °C
title_full	Influence of Zinc and Manganese Nanoparticles on Selected Parameters of Turkey Spermatozoa Stored in a Liquid State at 4 °C
title_fullStr	Influence of Zinc and Manganese Nanoparticles on Selected Parameters of Turkey Spermatozoa Stored in a Liquid State at 4 °C
title_full_unstemmed	Influence of Zinc and Manganese Nanoparticles on Selected Parameters of Turkey Spermatozoa Stored in a Liquid State at 4 °C
title_sort	influence of zinc and manganese nanoparticles on selected parameters of turkey spermatozoa stored in a liquid state at 4 °c
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bc5135b85cad471e8c768a8b41d51e21
work_keys_str_mv	AT aleksandraorzołek influenceofzincandmanganesenanoparticlesonselectedparametersofturkeyspermatozoastoredinaliquidstateat4c AT katarzynatrafalska influenceofzincandmanganesenanoparticlesonselectedparametersofturkeyspermatozoastoredinaliquidstateat4c AT wiktoriaaotowska influenceofzincandmanganesenanoparticlesonselectedparametersofturkeyspermatozoastoredinaliquidstateat4c AT władysławkordan influenceofzincandmanganesenanoparticlesonselectedparametersofturkeyspermatozoastoredinaliquidstateat4c AT annajkorzekwa influenceofzincandmanganesenanoparticlesonselectedparametersofturkeyspermatozoastoredinaliquidstateat4c AT krzysztofkozłowski influenceofzincandmanganesenanoparticlesonselectedparametersofturkeyspermatozoastoredinaliquidstateat4c
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Influence of Zinc and Manganese Nanoparticles on Selected Parameters of Turkey Spermatozoa Stored in a Liquid State at 4 °C

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